CHARACTERIZATION OF EMERGING BLUETONGUE VIRUS SEROTYPE 3 STRAINS IN THE MEDITERRANEAN BASIN BY NEXT GENERATION SEQUENCING

master's thesis

• Title (croatian): TIPIZACIJA EMERGENTNOG VIRUSA BOLESTI PLAVOG JEZIKA SEROTIPA 3 NA PODRUČJU MEDITERANA KORIŠTENJEM METODE SEKVENCIRANJA SLJEDEĆE GENERACIJE (NGS)
• Author: Lucija Jurišić
• Mentor: Ljubo Barbić (mentor)
• Committee member: Dean Konjević (predsjednik povjerenstva)
• Committee member: Zoran Milas (član povjerenstva)
• Committee member: Ljubo Barbić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Microbiology and Infectious Diseases with Clinic); Zagreb
• Defense date and country: 2019-10-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Public Health and Food Safety

Abstract

Bluetongue (BT) is a vector-borne viral OIE-listed disease of wild and domestic ruminants caused by bluetongue virus (BTV). BT has severe economic repercussions for the livestock industry due to direct losses caused by the infection but also due to indirect losses as a result of restrictions on animal trade. Since 1998, Southern Europe and the Mediterranean basin have experienced multiple incursions of different strains belonging to different serotypes and topotypes of BTV. In 2016 a BT outbreak occurred in Tunisia when the etiological agent was characterized as a novel western BTV serotype 3 strain. One year after the notification in Tunisia, BTV-3 outbreaks were evidenced in Sicily and Sardinia.
Considering the history of the epidemiology of BTV in the Mediterranean basin and the absence of specific vaccines, a novel BTV-3 certainly represents a risk for the European livestock industry. Therefore, in this thesis several aspects were analyzed including the development of a specific and accurate real time RT-PCR for the detection of BTV-3 RNA, the analysis of BTV-3 spread by means of virological and serological methods within the particular emphasis on a genome characterization of BTV-3 using a next generation sequencing (NGS), a novel molecular diagnostic technique.

Abstract (croatian)

Bolest plavog jezika (BPJ) je vektorska bolest divljih i domaćih preživača koja se prenosi virusom bolesti plavoga jezika i koja se nalazi na listi bolesti Svjetske organizacije za zdravlje životinja (OIE). BPJ ima ozbiljne ekonomske posljedice za stočarsku industriju zbog izravnih gubitaka uzrokovanih infekcijom, ali i zbog neizravnih gubitaka kao posljedica ograničenja u trgovini životinjama. Od 1998. godine na području južne Europe i Mediterana došlo je do višestrukih izbijanja bolesti uzrokovanih različitim serotipovima i topotipovima virusa BPJ. 2016. godine došlo je do epidemije BPJ u Tunisu, kada je etiološki agens okarakteriziran kao novi soj virusa bolesti plavog jezika -serotip 3. Godinu dana nakon izbijanja u Tunisu, epidemije virusa bolesti plavog jezika serotipa 3 zabilježene su na Siciliji i Sardiniji.
Obzirom na epidemiološku situaciju pojave virusa BPJ na Mediteranu i na odsutnost specifičnih cjepiva za novi serotip bolesti, navedeni serotip 3 virusa BPJ zasigurno predstavlja rizik za europsku stočarsku industriju. Stoga je u ovoj tezi analizirano nekoliko aspekata, uključujući razvoj specifičnog i preciznog RT-PCR-a u stvarnom vremenu za detekciju RNA serotipa 3 virusa BPJ te širenje serotipa 3 virusa BPJ pomoću viroloških i seroloških metoda s naglaskom na tipizaciju genoma serotipa 3 virusa BPJ korištenjem najnovije tehnike molekularne dijagnostike- sekvenciranja sljedeće generacije (NGS).

• Keywords: Bluetongue; Mediterranean basin; BTV serotype 3; NGS
• Keywords (croatian): Bolest plavog jezika; Mediteran; serotip 3 virusa BPJ; NGS
• Language: english
• URN:NBN: urn:nbn:hr:178:495013
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-11-11 12:03:23