Učinci esencijalnih ulja limuna i komorača te prirodnog zeolita na morfometrijske značajke krvnih stanica pilića

master's thesis

• Title (english): Effects of Supplemental Citrus Limonum and Foeniculum Vulgare Essential Oils and Natural Zeolite on Morphometric Characteristics of Chicken Blood Cells
• Author: Martina Triskoli
• Mentor: Nina Poljičak-Milas (mentor)
• Mentor: Kristina Matković (mentor)
• Committee member: Romana Turk
• Committee member: Kristina Matković
• Committee member: Nina Poljičak-Milas
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Pathophysiology); Zagreb
• Defense date and country: 2015-12-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Aktivne komponente mnogih biljaka koriste se kao antibakterijska i antioksidacijska
sredstva u borbi protiv raznih okolišnih stresora, oksidacije i potencijalno štetnih slobodnih
radikala. U stresnim uvjetima intenzivnog uzgoja životinja adaptivni mehanizmi stanica dovest
ce do promjena, povecanja ili nepravilnog izgleda stanica i jezgara, koje se potom mogu
kvantificirati morfometrijski. Morfometrijski pokazatelji eritrocita, limfocita i monocita mjereni
su 21. i 42. dana u kontrolnoj skupini pilica kao i u pokusnoj skupini kojoj su u hranu dodana
etericna ulja Aroma korm® (limuna i komoraca) te prirodni zeolit. Na ukupno 36 krvnih
razmaza mjerena je površina, opseg, minimalni polumjer, maksimalni polumjer, zakrivljenost
površine, dužina, širina te odnos jezgre i citoplazme (N/C). Digitalna analiza krvnih stanica
napravljena je na osobnom racunalu s podržavajucim programom SFORM (VAMSTEC, Zagreb,
Hrvatska). Rezultati prikazuju da su 21. dana tova uz dodatak etericnih ulja u hrani gotovo svi
pokazatelji eritrocita bili niži u usporedbi sa kontrolnom skupinom, dok su 42. dana vrijednosti
bile vece od kontrolnih. Porast velicine i nepravilan oblik limfocita i njihovih jezgara primjecuje
se u pokusnoj skupini 21. dana, dok su 42. dan svi izmjereni pokazatelji bili smanjeni u odnosu
na vrijednosti kontrolne skupine. Velicina monocita nije se mijenjala do 42. dana kada su imali
znatno manji opseg i zakrivljenost površine. Nakon pocetne reakcije na stres morfološke
promjene eritrocita ukazuju na intenzivniju eritropoezu u eksperimentalnih pilica. Smanjena
velicina i zakrivljenost površine limfocita i monocita 42. dana u eksperimentalnim skupinama
pilica posljedica je protuupalnog i antimikrobnog ucinka etericnih ulja komoraca i citrus limuna,
kao i djelovanja zeolita na smanjenje onecišcenja ukupne crijevne flore. Dobiveni rezultati
potvrdili su da morfometrijska analiza može pružiti vrijedne podatke u procjeni adaptivnih
odgovora krvnih stanica na stres te može nadopuniti uobicajenu hematološke pretrage.

Abstract (english)

Active components of many plants have evolved as antibacterial and antioxidant agents
against environmental stress due to a variety of oxidizing and potential harmful free radicals.
Adaptive mechanisms of cells to stress conditions lead to changes, increase and/or abnormalities
of cells and nuclei, that can be quantified by morphometry. Morphometric parameters of
erythrocytes, lymphocytes and monocytes were measured on the 21st and on the 42nd day in the
control group, and in experimental group of chicken fattening with essential oils Aroma korm®
and clinoptilolite as food additives. On the total of 36 blood smears area, outline, minimum and
maximum radius, convexity, length and breadth of the whole cells and their nuclei as well as
nucleo/cytoplasmic ratio were measured. Digital image analysis was performed using the
SFORM software (VAMSTEC, Zagreb, Croatia). On the 21st day of fattening with essential oils
as food additives almost all measured erythrocyte parameters were lower in comparison with the
control group, while on the 42nd day values increased and exceeded the control values. Increased
size and irregular shape of lymphocytes and their nuclei were seen in experimental group on the
21st day, but on the 42nd day all measured parameters were reduced in comparison to the values
of control group. The size of monocytes did not change until the 42nd day, when they had
significantly smaller outline and convexity. Established morphological changes of erythrocytes
indicate, after the initial stress reaction, more intensive erythropoiesis in experimental chickens.
Reduced size and convexity of lymphocytes and monocytes on the 42nd day in experimental
groups of chickens were in the accordance with anti-inflammatory and antimicrobial effects of
fennel and lemon essential oils, as well as zeolite effectiveness in reducing total flora
contamination on the intestine. Obtained results confirmed that morphometric analysis can
provide valuable data for assessing blood cell adaptive responses and could complement
conventional hematology.

• Keywords: stres; etericna ulja; morfometrija; eritrociti; limfociti; monociti. 38
• Keywords (english): stress; essential oils; morphometry; erythrocyte; lymphocyte; monocyte
• Language: croatian
• URN:NBN: urn:nbn:hr:178:029253
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-01-18 10:40:16