Antimikrobni, antioksidativni i imunomodulacijski učinak tekućih ekstrakata propolisa

master's thesis

• Title (english): ANTIMICROBIAL, ANTIOXIDANT AND IMMUNOMODULATORY EFFECT OF LIQUID PROPOLIS EXTRACTS
• Author: Jana Rak
• Mentor: Jelena Šuran (mentor)
• Mentor: Josipa Vlainić (mentor)
• Committee member: Frane Božić (predsjednik povjerenstva)
• Committee member: Andreja Prevendar Crnić (član povjerenstva)
• Committee member: Jelena Šuran (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2020-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Public Health and Food Safety

Abstract

Cilj ovog istraživanja bio je usporedba koncentracije deset markera biološke aktivnosti
(biomarkera) u šest različitih tekućih ekstrakata propolisa, alkoholnih (EEP1-3) i bezalkoholnih
(BEP1-3), s antimikrobnim i antioksidativnim učinkom. Koncentracija deset biomarkera
utvrđena je metodom tekućinske kromatografije visoke djelotvornosti s UV-Vis detektorom
(HPLC). U obje vrste otopina (EEP i BEP) najzastupljeniji biomarkeri jesu p-kumarinska i
trans-ferulinska kiselina, dok su kempferol i trans-cimetna kiselina najmanje zastupljeni
biomarkeri. Koncentracija najzastupljenijih biomarkera veća je u BEP-a, nego u EEP-a. Nije
utvrđena povezanost koncentracije biomarkera i postotka otopljenog propolisa u obje vrste
tekućih ekstrakata propolisa. Antimikrobni učinak šest ekstrakata propolisa određen je
mjerenjem MIK-a i MBEK-a. Najbolji antimikrobni učinak (najniže vrijednosti MIK80 i
MBEK) ima bezalkoholni ekstrakt BEP3. Provođenjem FRAP i DPPH metode određen je
antioksidativni učinak ekstrakata propolisa. U ovom istraživanju BEP u odnosu na EEP,
općenito su pokazali bolji antioksidativni učinak (osim BEP2).

Abstract (english)

The aim of this study was to compare the concentration of ten markers of biological activity
(biomarkers) in six different liquid propolis extracts, alcoholic (EEP1-3) and non-alcoholic
(BEP1-3), with antimicrobial and antioxidant effects. The concentration of ten biomarkers was
determined by high performance liquid chromatography with a UV-Vis detector (HPLC). In
both types of solutions (EEP and BEP) the most common biomarkers are p-coumaric and transferulic acid, while kaempferol and trans-cinnamic acid are the least represented biomarkers.
The concentration of the most common biomarkers is higher in BEPs than in EEPs. No
association was found between the biomarker concentration and the percentage of dissolved
propolis in both types of liquid propolis extracts. The antimicrobial effect of six propolis
extracts was determined by measuring MIK and MBEC. The best antimicrobial effect (lowest
values of MIK80 and MBEC) was shown by non-alcoholic extract of BEP3. The antioxidant
effect of propolis extracts was determined by performing FRAP and DPPH methods. In this
study, BEP compared to EEP, generally showed a better antioxidant effect (except BEP2)

• Keywords: biomarkeri; etanolni ekstrakt propolisa; bezalkoholni ekstrakt propolisa; antimikrobni učinak; antioksidativni učinak
• Keywords (english): biomarkers; ethanol propolis extract; non-alcoholic propolis extract; antimicrobial effect; antioxidant effect
• Language: croatian
• URN:NBN: urn:nbn:hr:178:543852
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-08-22 09:51:28