Diferencijalna krvna slika kokoši lake pasmine nakon terapije tekuti pripravkom fluralaner

master's thesis

• Title (english): Differential leukocyte count in light breed hens after treating poultry mites with fluralane
• Author: Gabriel Kontrec
• Mentor: Željko Gottstein (mentor)
• Mentor: Jasna Aladrović (mentor)
• Committee member: Lana Pađen (predsjednik povjerenstva)
• Committee member: Željko Gottstein (član povjerenstva)
• Committee member: Jasna Aladrović (član povjerenstva)
• Committee member: Liča Lozica (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2023-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Public Health and Food Safety

Abstract

Tekut peradi jedna je od najznačajnih parazitarnih bolesti koja se javlja u intenzivnom
uzgoju peradi. Postoji mnogo vrsta tekuti, no najučestalija je kokošja tekut (Dermanyssus
gallinae). Ova tekut putem ugriza neposredno ugrožava životinjsko zdravlje uzrokujući stres,
bol, kožne iritacije i pad proizvodnje, no može ugrožavati i posrednim putem kao prijenosnik
bolesti. Za suzbijanje tekuti koristili su se različiti biološki akaricidni pripravci, no nisu se
pokazali uspješnima stoga se počeo koristiti pripravak na bazi fluralanera (Exzolt, MSD).
Fluralaner djeluje kao inhibitor živčanog sustava artropoda s antagonističkim djelovanjem na
GABA receptore i receptore za glutamat i ujedno zaustavlja proizvodnju jajašaca kod ženki.
Cilj ovog istraživanja bio je monitoring ukupnog broja leukocita, te relativnog i apsolutnog
broja pojedinih loza i omjera heterofila i eozinofila (H/L) kod kokoši nesilica lake linije držanih
slobodno u volijerama. Terapija peradi započela je s 38 tjedana starosti te je pripravak davan
prema uputama proizvođača dva puta u razmaku od sedam dana u dozi od 0,5 mg fluralanera
po kilogramu jedinke per os putem vode za piće. Krv je uzorkovana punkcijom vratne vene
neposredno prije terapije (0. dan) te 7., 14., 21. i 60. dana nakon početka terapije. Dobiveni
rezultati pokazuju da je došlo do smanjenja ukupnog broja leukocita, limfocita, heterofila i
eozinofila, blagog porasta broja monocita te da se broj bazofila nije značajno mijenjao.
Promjene u omjeru heterofila i leukocita ukazuju na potencijalnu aktivaciju infekta na početku
terapije te na oporavak u kasnijoj fazi terapiranja. Terapija kokoši nesilica fluralanerom dovela
je do oporavka jedinki i povratka hematoloških parametara unutar fizioloških granica te
poboljšanja dobrobiti i proizvodnosti.

Abstract (english)

Poultry mites is one of the most important parasites in intensive poultry farming. There
are many different types of mites, but the most common one is red poultry mite (Dermanyssus
gallinae). This mites' bite indirectly puts animal health in danger by causing stress, pain, skin
irritations and production decline, but it can also can also endanger the animals directly as a
disease carrier. Many different acaricides have been used in order to suppress mite invasions,
but they haven't been proven successful. Therefore a new drug, fluralaner (Exzolt, MSD) is
being used. Fluralaner works as an inhibitor of the arthropodes' nerve system with an
antagonistic action on GABA receptors and glutamate receptors and also stops egg production
in females. The purpose of this study was to monitor the total number of leukocytes, the relative
and apsolute number of individual lineages and the heterophile and eozinophile (H/L ratio) in
laying hens kept in free range aviary systems. The terapy began at 38 weeks of age and the
drug was administred according to the manifacturer's instructions, twice, seven days apart in a
dose of 0,5 mg fluralaner per 1 kilogram of bodyweight orally via drinking water. The blood
was sampled by jugular venipuncture before the therapy (day 0.) and then 7., 14., 21. and 60.
day after treatment. The results have shown a decrease in the number of leukocytes,
lymphocytes, heterophiles and eozinophiles, the number of monocytes has slightly increased,
while the number of basophiles hasn't significantly changed. The changes in the H/L ratio
indicate on a potential germ activation on the beginning of therapy and in later stages of therapy
on the recovery. The laying hens have fully recovered from the mite invasions and
hematological paramethers have been returned to their normal values.

• Keywords: kokoši lake pasmine; tekut; fluralaner; diferencijalna krvna slika
• Keywords (english): hens of light breed; mites; fluralaner; differential blood count
• Language: croatian
• URN:NBN: urn:nbn:hr:178:225934
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-10-11 13:26:06