Patološka, parazitološka i molekularna istraživanja verminoznih pneumonija mačaka

master's thesis

• Title (english): Pathological, parasitological and molecular research of feline verminose pneumonias
• Author: Ema Gagović
• Mentor: Ana Beck (mentor)
• Mentor: Relja Beck (komentor)
• Committee member: Andrea Gudan Kirilj
• Committee member: Branka Artuković
• Committee member: Ana Beck
• Committee member: Relja Beck
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Veterinary Pathology); Zagreb
• Defense date and country: 2016, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Basic and Preclinical Veterinary Sciences

Abstract

Verminozne pneumonije mačaka su kronične upale pluća uzrokovane oblićima iz rodova Aelurostrongylus i Troglostrongylus. Često su sporedan nalaz obdukcije, a podaci o stvarnoj učestalosti ove bolesti u populaciji mačaka u svijetu i u Hrvatskoj su dvojbeni. Morfološke sličnosti ličinki (L1) oblića Troglostrongylus spp. i A. abstrusus otežavaju razlikovanje ovih vrsta parazita. Obzirom da je u RH provedeno samo jedno istraživanje verminozne pneumonije mačaka, uzročnici verminozne pneumonije u šest mačaka istraženi su patološkim, parazitološkim i molekularnim metodama kako bi ih se identificiralo. Ovo istraživanje predstavlja prvu gensku potvrdu (18SrRNA, ITS2) A. abstrusus, dok isključuje prisutnost T. brevioru RH. Također predstavlja prvu primjenu umjetne probave u svrhu izolacije i determinacije plućnih nematoda.

Abstract (english)

Verminose pneumonias are chronic pneumonias caused by nematodes of the Aelurostrongylus and Troglostrongylus genus. They are often an incidental postmortal finding, and the data on the actual incidence of this disease in cats in the world and in Croatia are dubious. The morphological similarities of the larvae (L1) of the Troglostronylus spp. and A. abstrusus nematodes render differentiating these species difficult. As only one research of feline verminose pneumonia has been conducted in Croatia, the causal agents of verminose pneumonia in six cats have been subjected to pathological, parasitological and molecular investigation in order to identify them. This research presents the first genetic proof of (18SrRNA, ITS2) A. abstratus, while excluding the presence of T. brevior in Croatia. It also presents the first application of artificial digestion with the goal of isolating and determining these lung nematodes

• Keywords: mačka; verimozna pneumonija; A. abstrusus; T. brevior; histopatologija; citologija; molekularna tipizacija
• Keywords (english): feline; verminose pneumonia; A. abstrusus; T. brevior; histopathology; citology; molecular typing
• Language: croatian
• URN:NBN: urn:nbn:hr:178:912574
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-01-27 11:10:59