Istraživanje sustava intracitoplazmatskih granula u citološkim uzorcima pasa i mačaka

master's thesis

• Title (english): Investigation of the Composition of Intracytoplasmic Granules in Canine and Feline Cytological Samples
• Author: Neven Lončarić
• Mentor: Lidija Medven Zagradišnik (mentor)
• Mentor: Marko Hohšteter (mentor)
• Committee member: Andrea Gudan Kurilj (predsjednik povjerenstva)
• Committee member: Marko Hohšteter (član povjerenstva)
• Committee member: Lidija Medven Zagradišnik (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2024-09-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Provedenom studijom se istraživala mogućnost diferencijacije smeđih intracitoplazmatskih
granula u citološkim uzorcima bojenjem pruskim modrilom jer je sastav teško izdiferencirati
standardnim metodama bojanja. U istraživanju su korišteni arhivirani podatci i uzorci citoloških i
histopatoloških pretraga pasa i mačaka analizirani na Zavodu za veterinarsku patologiju,
Veterinarskog fakulteta u Zagrebu.
U istraživanju je pregledano 7667 arhiviranih uzoraka, a smeđe intracitoplazmatske
granule su utvrđene u 72 uzorka (0.93%) od kojih je 26 odbojano te ponovo obojano pruskim
modrilom. Pozitivan nalaz na prisustvo hemosiderinskog pigmenta je utvrđen u 21 uzorku. Najveći
dio uzoraka pozitivnih na prusko modrilo činili su uzorci kože (46.2%), a najčešća dijagnoza bila
je melanocitna neoplazija (23.8%). U 66.7% uzoraka pozitivnih na prusko modrilo
dijagnosticirano je više različitih vrsta pigmenata koje je klasičnim bojanjima gotovo nemoguće
izdiferencirati. Usporednom analizom utvrđena je podudarnost u 40.9% uzoraka između
postavljenih citoloških i histopatoloških dijagnoza, a nakon primjene pruskog modrila podudarnost
je porasla na 50% čime se potvrđuje korisnost ove dijagnostičke metode.
Rezultati ovog istraživanja su pokazali da se upotrebom pruskog modrila, na odbojenim
uzorcima analiziranim klasične metodama bojanja, može bolje diferencirati sastav pigmenata u
patološkim lezijama. Samim time se može znatno povećati točnost postavljenih dijagnoza, što
posebice dolazi do izražaja u diferencijaciji melanocitnih neoplazija bez dodatnih stresnih zahvata
na životinji i uz minimalne troškove za vlasnika. Posljedično tome rezultat ovakve pretrage može
biti od znatne koristi u dijagnostici, određivanju prognoze te terapiji bolesti.

Abstract (english)

The conducted study investigated the possibility of differentiation of brown
intracytoplasmic granules in cytological samples with the Prussian blue reaction, since the
composition is difficult to differentiate with standard staining methods. The research used archived
data and cytological and histopathological examinations of dogs and cats analyzed in the
Department of Veterinary Pathology, Faculty of Veterinary Medicine in Zagreb.
In the research, 7667 archived samples were examined, and brown intracytoplasmic
granules were determined in 72 samples (0.93%), of which 26 were selected and re-stained with
Prussian blue. A positive finding for the presence of hemosiderin pigment was found in 21
samples. Most of the samples positive for Prussian blue were skin samples (46.2%), and the most
common diagnosis was melanocytic neoplasia (23.8%). In 66.7% of samples positive for Prussian
blue, several different types of pigments were diagnosed, which are almost impossible to
differentiate with standard staining. The comparative analysis established the coincidence of the
cytological and histopathological diagnoses in 40.9% of the samples. After applying the Prussian
blue, the agreement increased to 50%, which confirms the usefulness of this diagnostic method.
The results of this research showed that the composition of pigments in pathological lesions
can be better differentiated by using Prussian blue on stained samples analyzed by classical
staining methods. This alone can significantly increase the accuracy of established diagnoses,
which is especially evident in the differentiation of melanocytic neoplasia without additional
stressful interventions on the animal and with minimal costs for the owner. The result of such a
test can be of considerable use in the diagnosis, determining the prognosis and therapy of the
disease.

• Keywords: citologija; smeđe granule; prusko modrilo; diferencijacija
• Keywords (english): cytology; brown granules; Prussian blue; differentiation
• Language: croatian
• URN:NBN: urn:nbn:hr:178:932804
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-17 12:47:18