Kvalitativno dokazivanje protutijela za parvovirozu i štenećak u pasa

master's thesis

• Title (english): Qualitative Detection of Antibodies Against Parvovirosis and Distemper in Dogs
• Author: Mija Curiš
• Mentor: Matko Perharić (mentor)
• Committee member: Zrinka Štritof (predsjednik povjerenstva)
• Committee member: Vilim Starešina (član povjerenstva)
• Committee member: Suzana Hađina (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2024-11-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Public Health and Food Safety

Abstract

Parvoviroza i štenećak kontagiozne su virusne zarazne bolesti pasa. Obje zarazne bolesti
proširene su diljem svijeta i imaju značajan utjecaj na zdravlje pasa. Prenose se izravnim i
neizravnim kontaktom sa zaražene na primljivu jedinku te se obje bolesti ističu visokim
letalitetom, pogotovo u mlađih dobnih kategorija. Pravilna imunoprofilaksa ključna je i
najučinkovitija mjera za sprječavanje pojave ovih zaraznih bolesti. Nakon provedenog
cijepljenja, većina pasa razvija zaštitni titar protutijela koji traje nekoliko godina. Cilj ovoga
istraživanja bio je utvrditi razinu imunološke zaštite protiv parvoviroze i štenećaka u pravilno
cijepljenih pasa kojima je od posljednjeg cijepljenja prošlo više od tri godine. Uzorci plazme
50 pasa pretraženi su komercijalno dostupnim testom Megacor FASTest® CDV-CPV Ab.
Uporabom navedenog testa utvrđen je stupanj zaštite za navedene bolesti dokazom protutijela
na virus parvoviroze i štenećaka. U svega 42% pasa (n=23) utvrđen je zaštitni titar protutijela
na parvovirus dok je 68% (n=34) pretraženih pasa imalo zaštitni titar na štenećak. Među
skupinom pasa kod kojih je od posljednjeg cijepljenja prošlo između tri i pet godina njih 52,38%
imalo je zaštitni titar na parvovirus, dok u skupini kod kojih je prošlo više od pet godina od
posljednjeg cijepljenja u 41,37% pasa je dokazan zaštitni titar na parvovirus. Zaštitni titar
protutijela za štenećak dokazan je u 66.66% pretraženih pasa posljednji put cijepljenih tri do
pet godina, odnosu u 68,96% pasa kod kojih je prošlo više od pet godina od zadnjeg cijepljenja.
Statističkom analizom skupina pasa necijepljenih tri do pet godina i skupine necijepljenih preko
pet godina nije dokazana statistički značajna razlika s obzirom na stupanj zaštite protiv
parvoviroze i štenećaka. Također, spol i pripadnost čistokrvnoj pasmini nisu bili povezani sa
stupanjem imunološke zaštite nakon cijepljenja pasa protiv parvoviroze i štenećaka. Rezultati
ovoga istraživanja ukazuju na potrebu utvrđivanja stupnja imunološke zaštite unutar tri godine
od posljednjeg cijepljenja pasa kako bi se pravovremeno provela odgovarajuća imunoprofilaksa
parvoviroze i štenećaka.

Abstract (english)

Canine parvovirosis and canine distemper are highly contagious viral diseases that pose
a significant threat to canine health globally. Both diseases are characterised by high morbidity
and mortality rates, especially in young dogs, and can be transmitted through both direct and
indirect contact with infected animals or contaminated environments. Effective
immunoprophylaxis, primarily through vaccination, remains the cornerstone of disease
prevention. Following a correctly administered vaccination protocol, most dogs retain
protective immunity for several years. The aim of this study was to determine the duration of
immunity in dogs that had been previously adequately vaccinated but had not received
additional vaccination for three years or more. Plasma samples from 50 dogs were analysed for
antibodies against parvovirosis and canine distemper virus by using the Megacor FASTest®
CDV-CPV Ab commercial test kit. Of all dogs, 42% (n=23) had a protective titre for canine
parvovirosis, while 68% (n=34) had a protective titre for canine distemper virus. Among the
group of dogs that had not received vaccination boosters in the last three to five years, 52.38%
had a protective antibody titre against parvovirus. In contrast, within the group of dogs that had
not been vaccinated for five or more years, only 41.37% retained a protective titre against
parvovirosis. Regarding immunity to canine distemper, 66.66% of dogs in the the three- to fiveyear post- vaccination group showed a protective antibody titre, while 68.96% of those
unvaccinated for five years or longer maintained a protective titre against distemper. Statistical
analysis revealed no statistically significant difference in protective immunity levels between
the two groups of dogs regarding parvovirosis and canine distemper. Furthermore, neither
gender nor breed was associated with the level of protective immunity against these diseases.
The findings of this study suggest that assessing protective immunity within three years of the
last vaccination is advisable to ensure timely immunoprophylaxis against parvovirosis and
canine distemper.

• Keywords: parvoviroza; štenećak; imunoprofilaksa; titar protutijela; trajanje imuniteta
• Keywords (english): parvovirosis; canine distemper; immunoprophylaxis; antibody titre; duration of immunity
• Language: croatian
• URN:NBN: urn:nbn:hr:178:110483
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-01-16 08:34:49