Aktivnost paraoksonaze 1 i koncentracija lipida u serumu i sjemenoj plazmi bikova simentalske pasmine

master's thesis

• Title (english): Paraoxonase 1 Activity and Lipid Concentrations in Serum and Seminal Plasma of Simmental Bulls
• Author: Edita Klepo
• Mentor: Jasna Aladrović (mentor)
• Committee member: Martina Lojkić (predsjednik povjerenstva)
• Committee member: Blanka Beer Ljubić (član povjerenstva)
• Committee member: Jasna Aladrović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Physiology and Radiobiology); Zagreb
• Defense date and country: 2017-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Cilj istraživanja bio je utvrditi aktivnost PON1 i koncentracije lipida u serumu i sjemenoj
plazmi simentalskih bikova ovisno o sezoni i dobi životinja. Uzorci krvi i sjemena bikova
uzimani su u ljeto i zimu. U serumu je određena aktivnost PON1, koncentracija ukupnog
kolesterola, kolesterola vezanog na lipoproteine velike gustoće (HDL-C) te triacilglicerola i
slobodnih masnih kiselina, a u sjemenoj plazmi aktivnost PON1 i koncentracije kolesterola i
triacilglicerola. Aktivnost PON1 i vrijednost omjera PON1/HDL-C u serumu bikova s
genomskim testom bile su značajno veće u oba godišnja doba u odnosu na progeno testirane
bikove. Koncentracija slobodnih masnih kiselina u uzorcima obje skupine bikova bila je
značajno veća u ljeto u odnosu na vrijednosti dobivene u zimu. Koncentracije kolesterola i
triacilglicerola u sjemenoj plazmi obje skupine bikova bile su značajno veće u zimskom
periodu u odnosu na ljeto. U ljetnim je uzorcima sjemene plazme progeno testiranih bikova
koncentracija kolesterola bila je značajno veća u odnosu na vrijednosti dobivene u sjemenoj
plazmi bikova s genomskim testom. Rezultati istraživanja potvrđuju da je serumska aktivnost
PON1 ovisna o dobi dok koncentracija NEFA ukazuje na povećanu mobilizaciju masti zbog
manjeg uzimanja hrane usljed visokih ljetnih temperatura.

Abstract (english)

The aim of this study was to investigate PON1 activity and lipid concentrations in serum and
seminal plasma of Simmental bulls according to seasonality and age. Blood samples and
bovine semen were obtained in summer and in winter. Serum PON1 activity, concentrations
of total cholesterol, high density lipoprotein cholesterol (HDL-C), triacylglycerols and
nonesterified fatty acids (NEFA) were determined; whereas seminal plasma PON1 activity
and concentrations of total cholesterol and triacylglycerols were analyzed. Activity of PON1
and PON1/HDL-C ratio in serum of bulls with genomic test were significantly higher in both
seasons compared with progeny tested bulls. The NEFA concentrations recorded in serum
samples of both group collected in summer was significantly higher compared with that in
winter. Concentrations of cholesterol and triacylglycerols in seminal plasma were
significantly higher in winter samples compared with summer samples in both groups. In
summer samples, seminal plasma concentrations of cholesterol and triacylglycerols of bull
with progeny test were significantly higher compared with that in bulls with genomic test. In
conclusion, we confirmed the relation between PON1 activity and the bulls age. The higher
summer NEFA concentrations show that bulls are less prone to eating due to high
temperatures during summer, and therefore use their body fat reserves.

• Keywords: bikovi; lipidi; serum; sjemena plazma; paraoksonaza 1
• Keywords (english): bulls; lipids; seminal plasma; serum; paraoxonase 1
• Language: croatian
• URN:NBN: urn:nbn:hr:178:144841
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-23 12:11:59