Utjecaj egzogenog melatonina na standardne pokazatelje kakvoće sjemena jarčeva izvan rasplodne sezone

master's thesis

• Title (english): Influence of Exogenous Melatonin on Standard Sperm Quality Parameters in Bucks Out of Breeding Season
• Author: Anja Ognjenović
• Mentor: Silvijo Vince (mentor)
• Mentor: Ivona Žura Žaja (mentor)
• Committee member: Marko Samardžija (predsjednik povjerenstva)
• Committee member: Silvijo Vince (član povjerenstva)
• Committee member: Ivona Žura Žaja (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Reproduction and Obstetrics Clinic); Zagreb
• Defense date and country: 2016-12-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Učinak egzogenog melatonina na spolni nagon (libido) i kakvoću sjemena jarčeva nije dostatno istražen, a podaci o učinku melatonina na plodnost ovnova su poprilično oprečni. Stoga je cilj ovoga rada bio istražiti učinak egzogenog melatonina na libido te standardne pokazatelje kakvoće sjemena jarčeva izvan rasplodne sezone. Jarčevi pasmine Francuska alpina (n=12) u dobi od 2 do 4 godine podijeljeni su u pokusnu i kontrolnu skupinu. Pokusnu su skupinu činili jarčevi (n=6) kojima je krajem ožujka aplicirano 4 melatoninska implantanta (74 mg), a kontrolnu jarčevi (n=6) bez melatoninskih implantata. Uzorci ejakulata uzimani su pomoću umjetne vagine jednom tjedno od ožujka do lipnja, a istovremeno se procjenjivao libido svakog jarca. Vremensko razdoblje trajanja pokusa od tri mjeseci podijeljeno je na 6 razdoblja, tako da je svaki mjesec podijeljen u prvu i drugu polovicu. U uzorcima je određen volumen i koncentracija ejakulata, masovno gibanje i gibanje spermija, udio živih i patoloških oblika spermija te su izračunati ukupni broj spermija i ukupni funkcionalni broj spermija u ejakulatu. Pokusna skupina jarčeva imala je značajno veće vrijednosti masovnog gibanja i gibanja spermija u prvoj polovici travnja te udjela živih spermija u prvoj i drugoj polovici travnja (P<0,05). Razina libida bila je veća u pokusnoj u odnosu na kontrolnu skupinu jarčeva tijekom cijelog pokusnog razdoblja, no razlike nisu bile značajne (P>0,05). Dobiveni rezultati ukazuju da egzogeni melatonin neposredno nakon aplikacije značajno pridonosi poboljšanju kakvoće ejakulata jarčeva (povećanju masovnog gibanja i gibanja spermija te udjelu živih spermija u ejakulatu). Blagotvorni učinak bi se moglo pripisati antioksidativnom djelovanju melatonina na spermije, što bi se trebalo detaljnije istražiti u daljnjim analizama.

Abstract (english)

The effect of exogenous melatonin on libido and quality of buck semen is not investigated sufficiently as yet, whereas data regarding the effect of melatonin on ram fertility were often controversial. Thus, the aim of this study was to investigate the effect of exogenous melatonin on libido and standard sperm quality parameters in bucks out of breeding season. Twelve bucks of French alpine breed aging from 2-4 years were assigned into experimental and control group comprising 6 bucks each. Total duration of the experiment of 3 months from March to June was divided into 6 periods in a way that each months was divided into the first and second half. The experimental group of bucks received 4 melatonin implants (74 mg) at the end of March, while the control group of bucks was tested without melatonin implants. Semen samples were taken from all bucks by artificial vagina once per week and simultaneously their libido was estimated. In the obtained samples volume and concentration of semen, massive motility and motility of spermatozoa, proportion of live and pathologic forms, total number of spermatozoa and total functional spermatozoa fraction were calculated. The experimental group of buck had significantly higher values of massive motility and motility of spermatozoa in the first half of April as well as the proportion of live spermatozoa in the first and second half of April (P<0.05). The intensity of libido was higher in the experimental than in the control group of bucks during the experimental period, but differences were not significant (P>0.05). The results indicated that the application of exogenous melatonin significantly improved the qualitative parameters of semen in bucks as established by increased massive motility, motility of spermatozoa and the proportion of live spermatozoa. These beneficial effects of melatonin could be ascribed to its antioxidative effects on spermatozoa. However, this finding should be studied in more details by further analyses.

• Keywords: kakvoća ejakulata; melatonin; libido; rasplodna sezona; jarčevi
• Keywords (english): ejaculate quality; melatonin; libido; breeding season; bucks
• Language: croatian
• URN:NBN: urn:nbn:hr:178:497580
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-23 12:53:07