Dokaz bakterija iz roda Salmonella u uzorcima podrijetlom od peradi različitim molekularnim metodama

master's thesis

• Title (english): Detection of Bacteria in Genus Salmonella using Different Molecular Methods in Samples Originated from Poultry
• Author: Stipe Brkić
• Mentor: Željko Gottstein (mentor)
• Mentor: Danijela Horvatek Tomić (mentor)
• Committee member: Estella Prukner-Radovčić (predsjednik povjerenstva)
• Committee member: Danijela Horvatek Tomić (član povjerenstva)
• Committee member: Željko Gottstein (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Poultry Diseases with Clinic); Zagreb
• Defense date and country: 2017-03-31, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Salmoneloze su zarazne bolesti ljudi i životinja uzrokovane bakterijama roda Salmonella. Zarazne bolesti uzrokovane salmonelama kod peradi možemo svrstati u 3 skupine. Prvu skupinu čine zaraze s dva negibljiva serovara, S. Pullorum (uzrokuje pulurozu) i S. Gallinarum (uzrokuje tifus). Drugu skupinu čine serovari S. Arizonae (najčešće u purana) dok u treću skupinu spadaju zaraze različitim serotipovima salmonela koje su najčešći uzročnici trovanja hranom u ljudi, te se jednim imenom nazivaju paratifusne infekcije. Zaražena perad jedan je od glavnih prirodnih rezervoara salmonela koje se konzumacijom zaraženog mesa ili jaja mogu prenijeti na ljude i uzrokovati trovanja, te je zbog toga jako važno kontrolirati sustave prizvodnje mesa i jaja podrijetlom od peradi. Brza i precizna metoda otkrivanja salmonela je stoga od izuzetne važnosti u prevenciji širenja zaraze. U ovom istraživanju smo usporedili LAMP i RealTime PCR metode u svrhu detekcije Salmonella u različitim uzorcima podrijetlom od peradi. LAMP se pokazao kao jednostavnija i brža metoda u usporedbi s RealTime PCR zbog bržeg očitovanja disocijacijske krivulje. Međutim, obje metode imaju visoku specifičnost i osjetljivost pa mogu dokazati vrlo male količine genetskog materijala u uzorku.

Abstract (english)

Salmonellosis is disease of humans and animals caused by bacteria of genus Salmonella. Infectious diseases caused by Salmonella in poultry can be divided into three groups. First group consists of infections by non-motile serovars S. Pollorum (causes pulorosis) and S. Gallinarum (causes typhus). Second group encompasses infections by S. Arizonae serovar (most common in turkeys), while the third group consists of diseases commonly called paratyphoid infections and are most typically seen in cases of food poisoning in humans and are caused by various salmonella serotypes. Infected poultry is one of the most important natural reservoir of Salmonella that could be transferred to humans by infected meat and egg consummation and cause poisoning. That is why it is very important to control system of production of meat and egg derived from poultry. Fast and precise method for Salmonella detection is therefore of outmost importance in prevention of infection spread. In this study, we compared LAMP and RealTime PCR methods for detection of Salmonella in different samples derived from poultry. We showed that LAMP method is simpler and faster comparing to Real Time PCR due to faster appearance of dissociation curve. Nevertheless, both methods have high specificity and sensitivity and are able to detect very small amounts of genetic material in the sample.

• Keywords: Salmonella; LAMP; RealTime PCR
• Keywords (english): Salmonella; LAMP; Real Time PCR
• Language: croatian
• URN:NBN: urn:nbn:hr:178:507685
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-24 11:03:53