Diferencijacija stanica u primarnoj kulturi neurona

master's thesis

• Title (english): Cell Differentiation in Primary Neuronal Culture
• Author: Mladen Mirić
• Mentor: Snježana Kužir (mentor)
• Mentor: Ivan Alić (mentor)
• Committee member: Tajana Trbojević Vukičević (predsjednik povjerenstva)
• Committee member: Martina Đuras (član povjerenstva)
• Committee member: Snježana Kužir (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Anatomy, Histology and Embriology); Zagreb
• Defense date and country: 2016-12-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Glavni cilj ovoga istraživanja bio je napraviti primarnu kulturu neurona podrijetlom iz
dva mišja soja kroz 7 i 14 dana, opisati uzorak izražaja bjelančevine te ih međusobno usporediti.
Stanice mišjeg soja GFP-LUC tijekom sedmog dana su se diferencirale u potpunosti u zrele
neurone i astrocite, od kojih je GFP pozitivan u 100% diferenciranih stanica, dok su biljezi
neurona (MAP2, ß3-tubulin i NeuN) pozitivni u 90% diferenciranih stanica, a biljeg astrocita
GFAP u samo 10%, odnosno tijekom četrnaestog dana su stanice razvijenije (veći broj i deblji
nastavci), a biljezi neurona su pozitivni u 80% diferenciranih stanica, a biljeg astrocita u 20%.
Stanice mišjeg soja Thy1-YFP tijekom sedmog dana su se također u potpunosti diferencirale u
zrele neurone i astrocite, međutim ovdje je samo 20% neurona YFP pozitivno. Kao i kod GFPLUC
mišjeg soja, i ovdje je u kulturi prisutno 90% neurona i 10% astrocita, odnosno 80%
neurona i 20% astrocita tijekom četrnaestog dana.

Abstract (english)

The main goal of this study was to differentiate a primary culture of neurons derived
from two different mouse strains through 7 and 14 days, to describe the pattern of protein
expression, and compare them with each other. Cells derived from GFP-LUC mouse strain
during the 7th day were completely differentiated into mature neurons and astrocytes and they
were 100% GFP positive; 90% were neurons and express neuronal markers (MAP2, ß3-tubulin
and NeuN) while the 10% were astrocytes and express GFAP. On the 14th day of differentiation
80% express neuronal markers while 20% express GFAP. On the other hand, cell derived from
Thy1-YFP mouse strain were also completely differentiated on the 7th day, but only 20%
neurons express Thy1-YFP. Similar, like GFP-LUC cells on the 7th day we observed 90%
neurons and 10% astrocytes in cell culture, while on the 14th day percentage of neurons decrease
at 80% while percentage of astrocytes increase on 20%.

• Keywords: GFP-LUC; Thy1-YFP; matične stanice; diferencijacija; transgenični miš
• Keywords (english): GFP-LUC; Thy1-YFP; stem cells; differentiation; transgenic mouse
• Language: croatian
• URN:NBN: urn:nbn:hr:178:061182
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-24 11:10:03