Ekspresija E-kadherina u tumorima apokrinih i lojnih žlijezda kože u pasa i povezanost s preživljavanjem

master's thesis

• Title (english): Expression of E-cadherin in Canine Tumors of Apocrine and Sebaceous Glands and Relationship with the Survival Rate
• Author: Ivana Mihoković
• Mentor: Marko Hohšteter (mentor)
• Committee member: Branka Artuković (predsjednik povjerenstva)
• Committee member: Ivan-Conrado Šoštarić-Zuckermann (član povjerenstva)
• Committee member: Marko Hohšteter (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Veterinary Pathology); Zagreb
• Defense date and country: 2017-04-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

U radu je istraţivana uĉestalost pojedinih histoloških tipova tumora apokrinih i lojnih ţlijezda u pasa ĉiji su uzorci dostavljeni na histopatološku dijagnostiku na Zavod za veterinarsku patologiju Veterinarskog fakulteta u Zagrebu, u razdoblju od 1. sijeĉnja 2010. do 31. prosinca 2012. godine. UtvrĊivano je da li postoji povezanost izmeĊu imunohistokemijske ekspresije E-kadherina i tipa tumora te da li postoji povezanost ekspresije E-kadherina vezano uz biološko ponašanje tumora.
Pasmina koja najĉešće obolijeva od tumora apokrinih i lojnih ţlijezda je zlatni retriver. Prosjeĉna dob oboljelih ţivotinja iznosila je 10,15 godina za tumore apokrinih ţlijezda, dok je za tumore lojnih ţlijezda iznosila 10,91 godina. Histopatološkom klasifikacija tumora utvrĊeno je 53,8% karcinoma i 46,2% adenoma apokrinih ţlijezda, a za tumore lojnih ţlijezda 50,0% epitelioma, 36,4% adenoma te 13,6% karcinoma. Vrijeme preţivljavanje pasa s tumorima lojnih ţlijezda je bilo najkraće kod epitelioma, dok je srednje preţivljavanje bilo kod adenoma i najduţe kod karcinoma. Metastaziranje je utvrĊeno kod malog broja adenoma i epitelioma, a uĉestalost recidiva je bila najveća kod karcinoma lojnih ţlijezda. U tumora apokrinih ţlijezda je utvrĊeno pribliţno podjednako vrijeme preţivljavanja za adenome i karcinome te ĉešće metastaziranje i recidiviranje karcinoma u odnosu na adenome. U tumora apokrinih ţlijezda je utvrĊeno pribliţno podjednako vrijeme preţivljavanja za adenome i karcinome te ĉešće metastaziranje i recidiviranje karcinoma u odnosu na adenome.
Imunohistokemijskom analizom ekspresije E-kadherina je dokazano da analiza ekspresije E-kadherina moţe koristiti u odreĊivanju toĉnije dijagnoze tipa tumora apokrinih, a osobito tumora lojnih ţlijezda pasa. Imunohistokemijskom analizom je utvrĊeno da kod tumora lojnih ţlijezda sama lokalizacija i intenzitet ekspresije E-kadherina mogu pomoći kod odreĊivanja prognoze, odnosno biološkog ponašanja tumora. Ekspresija E-kadherina u tumorima apokrinih ţlijezda pasa u provedenom istraţivanju nije pokazala rezultate koji bi upućivali na povezanost rezultata imunohistokemijske pretrage i biološkog ponašanja tumora.

Abstract (english)

In this study, incidence of histological types of the canine tumors of apocrine and sebaceos glands analyzed at Department of veterinary pathology in period between January, 1st 2010. and December, 31st 2012. was investigated. An association between immunohistochemical expression of E-cadherin, types and biological behavior of tumors were evaluated.
Breed most commonly affected with apocrine and sebaceous gland tumors was golden retriever. Average age of dogs diagnosed with apocrine gland tumor and sebaceous gland tumor was 10,15 years and 10,91 years, respectively. According to the histopathological analysis there were 53,8% of carcinomas and 46,2% of adenomas of apocrine glands. Analysis of sebaceous gland tumors showed epitheliomas as dominant tumor type (50,0%), followed by adenomas (36,4%) and carcinomas (13,6%). The survival period of dogs with sebaceous gland tumors was shortest for patients with diagnosed epitheliomas, intermediate for adenomas and longest for carcinomas. Incidence of metastasis was very low among adenoma and epithelioma groups, and frequency of recidivism was highest for sebaceous gland carcinoma. Average survival period was approximately the same for apocrine gland adenomas and carcinomas with higher metastasis and recidivism incidence in carcinoma group.
Immunohistochemical analysis of E-cadherin expression confirmed efficiency of this method for more accurate classification of histological types of apocrine and especially sebaceous gland tumors of dogs. Conducted immunohistochemical analysis showed that location and intensity of E-cadherin expression can be helpful for better prediction of the biological behavior of sebaceous gland tumors. Contrary to sebaceous gland tumors, immunohistochemical analyzis of apocrine gland tumors showed that there was no association between E-cadherin expression and biological behavior of apocrine gland tumors.

• Keywords: tumor; apokrine ţlijezde; lojne ţlijezde; E-kadherin; imunohistokemija
• Keywords (english): tumor; apocrine gland; sebaceous gland; E-cadherin; immunohistochemistry
• Language: croatian
• URN:NBN: urn:nbn:hr:178:715357
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-25 06:24:37