Raznolikost mitohondrijske DNK jelena lopatara iz Nacionalnog parka Brijuni

master's thesis

• Title (english): Diversity of Mitochondrial DNA in Fallow Deer from Narional Park Brijuni
• Author: Klara Marić
• Mentor: Magda Sindičić (mentor)
• Committee member: Alen Slavica (predsjednik povjerenstva)
• Committee member: Zdravko Janicki (član povjerenstva)
• Committee member: Magda Sindičić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Game Biology, Pathology and Breeding); Zagreb
• Defense date and country: 2018-09-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Jelen lopatar (Dama dama) jedna je od najvaţnijih vrsta divljaĉi u Europi, a u Hrvatskoj je
prisutan prvenstveno u gaterskim uzgojima, dok je slabije zastupljen u otvorenim lovištima i
farmskom uzgoju. Smatra se autohtonom vrstom koja je izumrla tijekom posljednjeg ledenog
doba, a dokazi upućuju da su podruĉja Iberije, Sicilije, Balkana i Anatolije bili glacijalna
utoĉišta vrste. Danas je u Turskoj jedina preostala autohtona populacija, dok su ostale
populacije u Europi nastale kao posljedica ljudskog djelovanja. U svrhu analize genske
raznolikosti jelena lopatara iz Nacionalnog parka Brijuni, izolirana je DNK iz 22 uzorka tkiva.
Analizirani su sljedovi kontrolne regije mitohondrijske DNK ukupne duţine 405 parova baza
te su utvrĊena dva haplotipa. Dobiveni sljedovi kontrolne regije mtDNK usporeĊeni su s
ostalim sljedovima pohranjenim u GenBank i pronaĊene su podudarnosti sa sekvencama iz
Njemaĉke kod oba haplotipa, a jedan se pojavljuje i u lopatara u MaĊarskoj i Italiji. S obzirom
na otoĉnu izoliranost, raznolikost mtDNK iz NP Brijuni je veća nego oĉekivana te je u skladu
s raznolikošću europskih kontinentalnih populacija lopatara.

Abstract (english)

Fallow deer (Dama dama) is one of the most important game species in Europe. In Croatia the
species is mostly kept in enclosures, while breeding in open hunting grounds or farms is rare.
It is considered that fallow deer is autochthonous in Europe, but went extinct during the last
ice age. Research suggest that Iberia, Sicilia, Balkan and Anatolia were glacial refugia of the
species, but today the only autochthonous population survived in Turkey, while all other
European populations appear to be result of reintroduction. The goal of our research was to
analyze genetic diversity in fallow deer from National park Brijuni, Croatia. DNA was
isolated from 22 tissue samples. A total of 405 base pairs of mitochondrial DNA control
region were sequenced and two haplotypes were detected. Control region sequences were
compared with ones from Genbank database and matches with sequences from Germany were
found in both haplotypes, while one of the haplotypes was also present in fallow deer in
Hungary and Italy. Considering island isolation, diversity of mtDNA of Dama dama from
National park Brijuni was higher than expected and in accordance with diversity of European
continental populations of fallow deer.

• Keywords: genska raznolikost; mitohondrijska DNK; kontrolna regija; jelen lopatar
• Keywords (english): genetic diversity; mitochondrial DNA; control region; fallow deer
• Language: croatian
• URN:NBN: urn:nbn:hr:178:414229
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-09-19 11:59:14