Metode procjene kvalitete ejakulata i rasplodnog potencijala vojnih radnih pasa

master's thesis

• Title (english): Breeding Soundness Examination of Military Breeding and Working Dogs Owned by the Ministry of Defense of the Republic of Croatia
• Author: Tena Propadalo
• Mentor: Iva Getz (mentor)
• Mentor: Martina Lojkić (mentor)
• Committee member: Nikica Prvanović Babić (predsjednik povjerenstva)
• Committee member: Iva Getz (član povjerenstva)
• Committee member: Martina Lojkić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Reproduction and Obstetrics Clinic); Zagreb
• Defense date and country: 2019-09-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Svrha ovog istraživanja bila je ustanoviti reproduktivni potencijal vojnih rasplodnih i radnih pasa Ministarstva obrane Republike Hrvatske (MORH). Istraživanje je provedeno na 16 spolno zrelih vojnih rasplodnih i radnih pasa na kojima je proveden klinički pregled, ultrazvučni pregled testisa, epididimisa i prostate te je ocjenjena kvaliteta ejakulata nakon polučivanja i nakon pohrane na 4 °C tijekom 24, 48 i 72 sata. Psi su podijeljeni u dvije dobne kategorije: < 2 godine (1,24 ± 0,19 godine) i > 2 godine (6,26 ± 2,48 godina). Za opći klinički i androloški pregled korišten je jednoobrazni obrazac. Nakon polučivanja ejakulata ocijenjen je volumen, pH, progresivna pokretljivost, koncentracija i vitalnost spermija (HOS test i bojenje po Bloom-u). Razrijeđeno sjeme pohranjeno je na 4° C kroz te su pokretljivost, vitalnost i integritet membrane ocjenjeni nakon 24, 48 i 72 sata.
Prosječna vrijednost obujma testisa, veličine prostate i tjelesne mase bila je statistički značajno veća u pasa starije dobne kategorije, što je pozitivno koreliralo s volumenom ejakulata i koncentracijom spermija u ejakulatu. Značajne, negativne korelacije zabilježene su između dobi pasa i progresivne pokretljivosti, integriteta membrane i normalne morfologije spermija. U pasa < 2 godine udio spermija normalne morfologije bio značajno veći u odnosu na pse > 2 godine. Abnormalnosti glave i srednjeg dijela spermija bili su značajno veći u pasa > 2 godine u odnosu na mlađe pse. Značajne korelacije postoje između pokretljivosti, HOS testa i rezultata supravitalnog bojenja po Bloom-u nakon pohrane sjemena kroz 72 sata. Pokretljivost nakon pohrane na 4 °C pozitivno korelira s progresivnom pokretljivošću nativnog sjemena
Ovim istraživanjem pokazalo se da je nužno uključiti niz pretraga, od kliničkih do laboratorijskih, kako bi dobili potpuni uvid u rasplodni potencijal pasa te za rasplod odabrali one najkvalitetnije. Rezultati ocjene polučenog ejakulata rasplodnih i radnih pasa MORH-a pokazali su da je ejakulat ovih pasa visokog rasplodnog potencijala, a odlični rezultati preživljavanja pohranjenih spermija na 4 °C nakon 24, 48 i 72 sata omogućavaju i međunarodnu trgovinu kvalitetnim genetskim materijalom.

Abstract (english)

The aim of this study was to evaluate the breeding soundness of military breeding and working dogs owned by the Ministry of Defense of the Republic of Croatia (MDRC). Sixteen sexually mature military dogs were subjected to a clinical examination, B-mode ultrasonography of the testes, epididymis and prostate, semen evaluation at collection and after storage at 4 ° C for 24, 48 and 72 h. Dogs were divided into two age categories < 2 years (1,24 ± 0,19 years) and > 2 years (6,26 ± 2,48 years). For clinical examination uniform database sheet was used. Semen was evaluated using the following parameters: colour, volume, pH, progressive motility, concentration and viability (HOS test and Bloom staining). Diluted semen samples were stored at 4 °C and the motility, viability and functional integrity of sperm membrane was evaluated after 24, 48 and 72 hours. Average testicular volume, prostate size and body weight were significantly higher in dogs > 2 years, which positively correlated with the semen volume and concentration of spermatozoa in the ejaculate. A negative correlations existed between the age of dogs and motility, membrane integrity (HOS test) and normal sperm morphology. In dogs < 2 years the percentage of normal spermatozoa was significantly higher compared to dogs > 2 years. Head and midpiece abnormalities were significantly higher in dogs > 2 years compared to younger dogs. Significant correlations were established between motility, HOS test results and supravital staining by Bloom after 72 hours of storage.
This research has shown that it is necessary to include variety of clinical and laboratory methods in order to get a complete insight into breeding potential of dogs and choose those with the highest quality characteristics for reproduction. Our results of semen quality evaluation at collection and after storage indicate high reproductive potential of military breeding and working dogs of MDCR and offer a possibility of international trade of high quality genetic material.

• Keywords: vojni rasplodni i radni psi; rasplodni potencijal; ocjena ejakulata
• Keywords (english): military breeding and working dogs; reproductive potential; semen quality
• Language: croatian
• URN:NBN: urn:nbn:hr:178:578251
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-01-28 12:05:36