Kanini kutani histiocitom: Citološke karakteristike - retrospektivni prikaz slučajeva dijagnosticiranih na Zavodu za veterinarsku patologiju 2009.-2018.

master's thesis

• Title (english): Canine Cutaneous Histiocytoma: Cytological Characteristics - Retrospective Study of Cases Diagnosed at the Department of Veterinary Pathology 2009.-2018.
• Author: Anja Kampuš
• Mentor: Ivan-Conrado Šoštarić-Zuckermann (mentor)
• Mentor: Andrea Gudan Kurilj (mentor)
• Committee member: Marko Hohšteter (predsjednik povjerenstva)
• Committee member: Ivan-Conrado Šoštarić-Zuckermann (član povjerenstva)
• Committee member: Andrea Gudan Kurilj (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Veterinary Pathology); Zagreb
• Defense date and country: 2019-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Cilj ovog rada bio je naglasiti važnost uzimanja uzoraka za citološku pretragu, bolje razumijevanje citoloških karakteristika kaninog kutanog histiocitma i povezanost upalnih stanica s tumorskom regresijom. Citološka pretraga vrlo je vrijedna dijagnostička metoda u dijagnostici kaninog kutanog histiocitoma zbog točnosti, brzine, jednostavnosti i prihvatljive cijene. Ovo istraživanje provedeno je na 20 citoloških preparata iz arhive Zavoda za veterinarsku patologiju Veterinarskog fakulteta Sveučilišta u Zagrebu. Iz uputnice koja prati svaki preparat prikupljeni su opći podaci o pacijentu i novotvorevini, a uključivali su: datum dostave, ime pacijenta, pasminu, dob, spol, lokalizaciju novotvorevine i dijagnozu. Mikroskopskom pretragom ponovno su pregledani preparati i određivane su citološke karakteristike koje uključuju staničnost, omjer jezgre i citoplazme, prisutnost anizocitoze i anizokarioze, infiltraciju limfocita i neutrofila, te prisutnost nekrotičnog debrisa. Rezultati ovog istraživanja podijeljeni su u dvije kategorije koje čine opći podataci o pacijentu i novotvorevini te njihove citološke karakteristike. U ovom istraživanju potvrđena su ranija saznanja drugih autora kao što su: povećana učestalost ovih tumora u mladih pasa, najčešće pojavljivanje na području glave, vrata i ekstremiteta i prisutnost pasminske predispozicije, dok citološke karakteristike potvrđuju izgled tumorskih stanica i prisutnost upalnih stanica koje su povezane s mogućnošću spontane regresije. Iako nismo koristili objektivne indikatore koji bi ukazivali na tumorsku regresiju (klinički parametri, dodatni imunohistokemijski markeri itd.), rezultati sugeriraju da su procesi koji ukazuju na regresiju povezani s povećanom infiltracijom limfocita, povećanim udjelom anizocitoze i anizokarioze, te smanjenim omjerom jezgre i citoplazme, dok infiltracija neutrofila nije jasno povezana s regresijskim promjenama.

Abstract (english)

The aim of this study was to emphasize the importance of sampling for cytological examination, a better understanding of the cytological characteristics of canine cutaneous histiocytoma, and the association of inflammatory cells with tumor regression. Cytological examination is a very valuable diagnostic method in the diagnosis of canine cutaneous histiocytoma because of its accuracy, speed, simplicity and reasonable price. This research was performed on 20 cytological preparations from the archive of the Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Zagreb. The samples were microscopically reevaluated. At the same time the following cytological characteristics were determinated: cellularity, nucleus to cytoplasm ratio, presence of anisocytosis and anisocariosis, infiltration of lymphocytes and neutrophils, and presence of necrotic debris. The results of this study were divided into two categories consisting of general patient and neoplasm data and their sample cytological characteristics. General patient and neoplasm data were collected from submission form accompanying each preparation and were including: date of delivery, patient name, breed, age, gender, neoplasm localization and diagnosis. This study confirmed earlier findings from other authors such as: increased incidence of these tumors in young dogs, most frequent occurrence in the head, neck and extremities appearances, and the presence of breed predisposition, while cytologic characteristics confirmed the appearance of tumor cells and the presence of inflammatory cells associated with the possibility of spontaneous regression. Although we did not use objective indicators to indicate tumor regression (clinical parameters, additional immunohistochemical markers etc.), the results suggested that regression related processes were associated with increased lymphocyte infiltration, increased anisocytosis and anisocariosis, and a decreased ratio of nucleus to cytoplasm, while neutrophil infiltration was not clearly associated with regression changes.

• Keywords: kanini kutani histiocitom; citologija; tumor; regresija; limfociti
• Keywords (english): canine cutaneous histiocytoma; cytology; tumor; regression; lymphocytes
• Language: croatian
• URN:NBN: urn:nbn:hr:178:410873
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-01-28 12:25:48