Citološka pretraga kutanih mastocitoma pasa u uzorcima obojenim may-grunwald-giemsa metodom i toluidinskim modrilom

master's thesis

• Title (english): Cytological Examination of Samples of Canine Cutaneous Mstcell Tumors Stained with May-Grunwald-Giemsa Method and Toluidine Blue Stains
• Author: Monika Perko
• Mentor: Andrea Gudan Kurilj (mentor)
• Mentor: Branka Artuković (mentor)
• Committee member: Marko Hohšteter (predsjednik povjerenstva)
• Committee member: Andrea Gudan Kurilj (član povjerenstva)
• Committee member: Branka Artuković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Veterinary Pathology); Zagreb
• Defense date and country: 2020-02-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Mastocitom je neoplazma podrijetla mastocita i najčešći je tumor kože u pasa.
Citološka pretraga je jedna od najbržih, najjeftinijih i najlakših dijagnostičkih postupaka koji se primjenjuje u kliničkom radu i može pomoći dijagnostičaru prilikom određivanja najprikladnije terapije prije operacije. Citološka dijagnostika mastocitoma temelji se na identifikaciji karakterističnih ljubičastih citoplazmatskih granula koje se boje metakromatski sa toluidinskim modrilom, giemsom i metilenskim modrilom. Cilj rada je bio 10 arhivskih uzoraka mastocitoma obojanih may-grünwald-giemsa metodom odbojiti te ponovo obojiti toluidinskim modrilom, a zatim tumore analizirati prema kriterijima za citološko graduiranje mastocitoma pasa. Nakon toga isti uzorci su uspoređeni sa stupnjem malignosti mastocitoma u histopatološkim preparatima obojenima hemalaun-eozin metodom. Rezultati su pokazali da se u citološkim razmascima obojenima toluidinskim modrilom bolje uočavaju postavljeni citološki kriteriji, a naročito detalji jezgre te bi- i multinuklearne stanice. Osim toga bolje se mogu diferencirati druge stanice (npr. fibroblasti, neutrofili i eozinofili), a eritrociti manje maskiraju ostale elemente. Može se zaključiti da bojenje toluidinskim modrilom omogućuje bolji uvid u kriterije za citološko graduiranje mastocitoma nego may-grünwald-giemsa bojenje te se prema tome može točnije odrediti citološki stupanj diferencijacije mastocitoma.

Abstract (english)

Mast cell tumors are neoplasms of mast cells origin and are the most common skin tumors in dogs. Cytologic examination is one of the fastest, cheapest and easiest diagnostic procedures used in clinical work that can help to clinician in determining the most appropriate therapy even before surgery. Cytologic diagnosis of mast cell tumors is based on the identification of characteristic purple cytoplasmic granules metachromatically stained with toluidine blue, giemsa and methylene blue. The aim of this study was to destain 10 archival cytological samples of mast cell tumors stained with may-grünwald-giemsa stain and restain it with toluidine blue. After this samples were analyzed according to the criteria for cytologic grading of mast cell tumors in dogs and the same samples were compared with the malignancy grade of mast cell tumors in histopathological sections stained with the hemalaun-eosin method. The results showed that smears stained with toluidine blue better detect the established cytological criteria, and in particular the nuclear details and bi- and multinuclear cells. In addition, other cells (eg. fibroblasts, neutrophils and eosinophils) are better visible, and erythrocytes less mask other elements. It can be concluded that staining with toluidine blue provides better insight into the criteria for cytologic grading of mast cell tumor than may-grünwald-giemsa staining, and thus the cytological degree of mast cell tumors can be more accurately determined.

• Keywords: mastocitom; pas; citološka pretraga; toluidinsko modrilo; stupanj malignosti
• Keywords (english): mast cell tumor; dog; cytology; toluidin blue; malignancy grade
• Language: croatian
• URN:NBN: urn:nbn:hr:178:054722
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-11-09 12:38:05