Dokaz i komparativna kvantifikacija sojeva Gallibacterium anatis i Mycoplasma synoviae iz obrisaka dušnika lakih i teških linija kokoši qPCR postupkom

master's thesis

• Title (english): Evidence and Comparative Quantification of Gallibacterium Anatis and Mycoplasma Synoviae Strains from Tracheal Swabs of Light and Heavy Chicken Breeds using qPCR Method
• Author: Silvia Buzaljko
• Mentor: Željko Gottstein (mentor)
• Committee member: Maja Lukač (predsjednik povjerenstva)
• Committee member: Danijela Horvatek Tomić (član povjerenstva)
• Committee member: Željko Gottstein (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Poultry Diseases with Clinic); Zagreb
• Defense date and country: 2020-10-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Mycoplasma synoviae najčešći je preduvjet za izbijanje drugih bakterijskih bolesti pri čemu se sojevi Gallibacterium anatis, koja je komenzal gornjih dišnih prohoda peradi, lako šire u donje dijelove dišnog sustava ili kroz jajovod ascedentno do abdominalne šupljine. M. synoviae uzrokuje subkliničku zarazu gornjih dišnih puteva, a može biti i sistemna zaraza uz narušavanje kvalitete jaja. Počinje kao respiratorna infekcija što uzrokuje upalu zračnih vrećica i dovodi do širenja sekundarnih respiratornih patogena. G. anatis izaziva pad nesivosti i slabiju kvalitetu jaja, javljaju se depresija i proljev te se u kroničnim slučajevima razvija purulentni peritonitis. U predisponirajuće čimbenike za nastanak bolesti, osim infekcije drugim mikroorganizmima, spadaju i genetika, stres, hormonski i metabolički poremećaji te neprikladne zoohigijenski uvjeti. Predmet ovog istraživanja bio je utvrditi prevalenciju ovih patogena na pretraživanim farmama i međusobnu korelaciju njihovog broja. Cilj je bio istražiti mogući sinergizam M.synoviae i G.anatis u prevalenciji na farmama. Istraživanje je provedeno na šesnaest jata podrijetlom s osam različitih farmi. Uzročnici su dokazani i kvantificirani qPCR metodom zbog svoje osjetljivosti i specifičnosti. Ova metoda omogućava ranu detekciju bolesti koja je preduvjet za izradu adekvatnog programa profilakse. U pretraživanim uzorcima postoji statistički značajna korelacija u prevalenciji oba patogena na pojedinim farmama kao i prosječno značajno veće količine G. anatis u uzorku, ali nema korelacije s obzirom na njihov broj odnosno količinu na farmi. Rezultati ove studije ukazuju na to da su potrebna daljnja ispitivanja i praćenje spomenutih uzročnika kako bi se osiguralo zdravlje peradi te dobra proizvođačka praksa.

Abstract (english)

Mycoplasma synoviae is the most common prerequisite for other microbial outbreaks. In that case G. anatis, which is commensal of the upper respiratory tract in poultry, spreads easily to the lower parts of the respiratory tract or through the reproductive system to abdominal cavity. M. synoviae causes subclinical infection of the upper respiratory tract as well as systemic infection with lower egg shell quality. It starts as a respiratory infection which causes airsacculitis and leads to development of other secondary respiratory pathogens. G.anatis causes drop in egg production and leads to lower egg shell quality, depression and diarrhea. Moreover, in cases of chronicle illness, purulent peritonitis is developed. Predisposing disease factors include genetics, stress, hormonal and metabolical disorders, unsuitable zoohygienic conditions along with infections caused by other microorganisms. The purpose of this study was to determine the correlation in prevalence of these two pathogens as well as mutual correlation of their quantity. The aim was to discover possible synergy between M.synoviae and G.anatis. Research has been done on sixteen flocks from eight different farms. The method of choice was qPCR because of its sensitivity and specificity. qPCR guaranty an early stage disease detection what is important for prophlylaxis development. There was statistically significant correlation in analyzed samples regarding prevalence of both pathogens on the farms with significantly higher average levels of G.anatis compared to M.synoviae. However, there was no correlation regarding their number. Therefore, the results of this study indicate that further research and monitoring should be carried out in order to ensure good flock health and production practice.

• Keywords: Mycoplasma synoviae; Gallibacterium anatis; qPCR; sinergizam; profilaksa
• Keywords (english): Mycoplasma synoviae. Gallibacterium anatis; qPCR; synergy; prophylaxis
• Language: croatian
• URN:NBN: urn:nbn:hr:178:132584
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-12-09 16:06:36