Pregled metoda konzerviranja životinjskih preparata u veterinarskoj anatomiji

master's thesis

• Title (english): Preview of Preservation Techniques for Animal Specimens used in Veterinary Anatomy
• Author: Silvija Jelačić
• Mentor: Tajana Trbojević Vukičević (mentor)
• Mentor: Martina Đuras (mentor)
• Committee member: Mirela Pavić (predsjednik povjerenstva)
• Committee member: Tajana Trbojević Vukičević (član povjerenstva)
• Committee member: Martina Đuras (član povjerenstva)
• Committee member: Srebrenka Nejedli (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Anatomy, Histology and Embriology); Zagreb
• Defense date and country: 2021-02-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Basic and Preclinical Veterinary Sciences

Abstract

Anatomski preparati su lešine ili dijelovi lešina koji su očuvani u svrhu provođenja znanstvenih istraživanja ili služe kao materijal za učenje te predstavljaju neizostavna pomagala u izučavanju anatomije. Ovisno o načinu korištenja preparata te zahtjevima kojima anatomski preparat mora udovoljavati, postoje različite specijalizirane metode fiksacije i konzerviranja. Pri Zavodu za anatomiju, histologiju i embriologiju Veterinarskog fakulteta Sveučilišta u Zagrebu, u svrhu izvođenja vježbi iz anatomije domaćih životinja, koriste se preparati konzervirani vodenom otopinom formaldehida (4 ili 10%), preparati impregnirani polietilenglikolom i svježi preparati. Zbog dokazanih toksičnih, alergijskih i kancerogenih svojstava formalina i s ciljem njegovog smanjenja i potpunog izbacivanja iz uporabe kao fiksativa, razvijene su brojne alternativne metode konzerviranja. Preparati konzervirani vodenom otopinom octene kiseline pokazali su se kao odlična zamjena formaliniziranim preparatima.Konzerviranjem octenom kiselinom nastaju anatomski preparati dobro očuvanih struktura te konzistencije, boje i izgleda sličnih nekonzerviranom tkivu. Metoda je jeftina i jednostavna za provođenje, njenim se korištenjem u relativno kratkom vremenu mogu proizvesti preparati spremni za upotrebu na anatomskim sekcijskim vježbama, za skladištenje nije potrebna specijalizirana dodatna oprema te održavanje preparata nije zahtjevno.

Abstract (english)

Anatomical specimens are whole carcasses or body parts that have been preserved for the purpose of scientific research or as learning material and are essential tools in the study of anatomy. Depending on the use of the anatomical specimen and the requirements it has to meet, there are different specialized techniques of fixation and preservation. Anatomical specimens used during anatomy course at the Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Zagreb, are fresh or have been preserved with formaldehyde solutions of different concentrations (4 or 10%), or impregnated with polyethylene glycol. Due to the proven toxic, allergic and carcinogenic characteristics of formalin there is a global trend to reduce and completely eliminate formalin as a fixative. Numerous alternative techniques of preservation have been developed during the years. Specimens preserved with concentrated acetic acid solutions have proven to be a good alternative to formalized specimens. The method is safe to use, non-toxic or health hazard. Additionally, it keeps good consistency, color and shape of the tissue. The method is cheap and easy to implement, so anatomical specimens for the dissection can be produced in a relatively short time, no additional equipment is required for storage and maintenance is not demanding.

• Keywords: anatomski preparati; konzerviranje; polietilenglikol; octena kiselina; formaldehid
• Keywords (english): anatomical specimens; preservation; polyethylene glycol; acetic acid; formaldehyde
• Language: croatian
• URN:NBN: urn:nbn:hr:178:912998
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2021-02-26 09:38:48