Kreiranje vrsno specifičnih početnica za umnažanje mitohondrijske DNA jelena lopatara (Dama dama)

master's thesis

• Title (english): Design of Species Specific Primers for Amplification of Fallow Deer (Dama dama) Mitochondrial DNA
• Author: Ines Vranešević
• Mentor: Tomislav Gomerčić (mentor)
• Mentor: Magda Sindičić (mentor)
• Committee member: Dean Konjević
• Committee member: Maja Popović
• Committee member: Tomislav Gomerčić
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Biology); Zagreb
• Defense date and country: 2014-12-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Basic and Preclinical Veterinary Sciences

Abstract

Ciljevi ovog rada bili su dizajnirati vrsno specificne pocetnice za umnažanje
kontrolne regije mitohondrijske DNA jelena lopatara (Dama dama), te optimizirati PCR
protokol za korištenje tih pocetnica. Pocetnice su dizajnirane temeljem sekvenci jelena
lopatara preuzetih iz genske baze podataka GenBank uz korištenje WEB aplikacije za
izradu pocetnica. Odredena je duljina pocetnica, mjesto vezivanja i duljina PCR
produkta. Pocetnice se koriste za identifikaciju jelena lopatara i razlikovanje od ostalih
vrsta, koristeci samo izolaciju DNA, PCR i elektroforezu. Upotreba pocetnica ispitana je
na osam uzoraka jelena lopatara, tri uzorka jelena obicnog i tri uzorka srne.Tako
dizajnirane vrsno specificne pocetnice moguce je primjenjivati u ekološkim
istraživanjima, istraživanjima prehrane predatora, u forenzickim slucajevima i u kontroli
mesnih namirnica u prehrambenoj industriji.

Abstract (english)

Goals of this work were to design species specific primers for amplification of
control region of mitochondrial DNA for fallow deer (Dama dama) and to find optimal
PCR protocol for application of those primers. Primers have been designed based on
fallow deer sequences from GenBank using the WEB application for primer design. I
have defined the size, location of and the size of the PCR product. With these specific
primers fallow deer can be identified and distinguished from the other animal species
using only DNA isolation, PCR and electrophoresis. Application of the primers has been
tested on eight samples of fallow deer, three samples of roe deer and three reed deer
samples. These species specific primers can be applied in ecological researches,
researches of predators feeding habits, in forensic cases and in control of game meat
products in food industry.

• Keywords: pocetnica; mitohondrijska DNA; PCR protokol; kontrolna regija; jelen lopatar
• Keywords (english): primer; mitochondrial DNA; PCR protocol; control region; fallow deer
• Language: croatian
• URN:NBN: urn:nbn:hr:178:455970
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-01-18 09:14:55