Referentne vrijednosti hematoloških i biokemijskih parametara u neonatalne teladi simentalske pasmine držane u sustavu krava-tele

master's thesis

• Title (english): Hematology and biochemistry reference interval for neonatal Simmental calves in cow-calf operation
• Author: damjan džakula
• Mentor: Darko Grden (mentor)
• Mentor: Jelena Gotić (mentor)
• Committee member: Martina Crnogaj (predsjednik povjerenstva)
• Committee member: Vladimir Mrljak (član povjerenstva)
• Committee member: Blanka Beer Ljubić (član povjerenstva)
• Committee member: Iva Šmit (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2023-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Neonatalna telad u uzgojima s dobrom farmskom praksom nalazi se pod intenzivnog nadzorom
te se u sklopu kontrole prijenosa pasivnog imuniteta uzorkuje krv teladi tijekom prvih 5 dana
života. Osim ukupnih proteina, ostatak krvnih parametara najčešće se ne određuje u uzetom
uzorku. Novija istraživanja potvrđuju postojanje razlika u vrijednostima krvnih parametara
među različitim pasminama, ali i dobnim kategorijama.
Cilj ovog istraživanja je određivanje hematoloških i biokemijskih referentnih intervala za
neonatalnu simentalsku telad do 5 dana starosti, kako bi iz svakog uzorka krvi sakupili
maksimalnu količinu informacija.
Prilikom usporedbe RV iz našeg istraživanja i RV iz recentnih publikacijama nalazimo razlike
u RV za telad iz našeg istraživanja i teladi slične dobi ali drugih pasmina. U usporedbi s ranijim
istraživanjima, u našem radu nalazimo visoku gornju granicu TP i ekstremno nisku gornju
granicu za BHB, dok za GGT nalazimo najvišu gornju granicu do sada objavljenu. Visoke
razine TP i GGT u serumu teladi pokazatelji su uspješnog prijenosa kolostralne imunosti. Prema
preporuci „Američkog konsenusa“ o prijenosu pasivne imunosti, naša telad pripada u „skupinu
izvrsnog prijenosa imunosti“, a taj status potvrđen je i visokim vrijednostima GGT koje
nalazimo. Niske razine BHB koje nalazimo u teladi ovog istraživanja, pokazatelj su visoke
razine dobrobiti, koja je rezultat dobre farmske prakse, naročito njege i postupanje s
neonatalnom teladi i njihovim majkama.
Referentni intervali određeni ovim istraživanjem mogu se koristiti za interpretaciju
hematoloških i biokemijskih parametara u slobodno držane neonatalne simentalske teladi u
sustavu krava-tele.

Abstract (english)

Neonatal calves in good farm practice systems are subject to constant monitoring, and as a part
of “passive immunity transfer monitoring” a sample of their blood is taken during the first 5
days of their life. Apart from the total protein (TP) the remaining blood profile parameters are
not adequately analyzed. Recent research has confirmed the existence of differences in normal
blood parameters between different breeds and age categories of the same species. The
objective of our study is to determine “de novo” hematology and biochemistry reference
intervals for Simmental neonatal calves up to five days of age, so that more information may
be extrapolated from every blood sample taken.
In comparison with published data in our research we found a high upper limit for TP and
extremely low upper limit for BHB, as for GGT we found the highest upper limit ever reported,
to the best of our knowledge. Comparing our results with the results of similar research we
found differences between our RI and published RI for similarly aged calves of different breeds.
High levels of TP and GGT in calf serum are indicators of successful colostral immunity
transfer. According to consensus recommendations for passive immunity transfer, the calves
from our study belong in the “excellent transfer group”, and that status is even further confirmed
by high GGT levels. We believe that the low concentrations of BHB found in our calves is an
indicator of a high level of animal welfare, a result of good farm practice related to the care and
handling of neonatal calves.
The RI found in our research can be used for interpretation of hematology and biochemistry
parameters in neonatal Simmental calves raised in free-range cow-calf operations.

• Keywords: hematološki i biokemijski pokazatelji; referentne vrijednosti; neonatalna telad; simentalac; sustav krava-tele
• Keywords (english): hematology and biochemistry values; reference intervals; neonatal calves; Simmental; cow-calf operation
• Language: croatian
• URN:NBN: urn:nbn:hr:178:871250
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-03-07 14:21:03