Diferencijalna krvna slika u kokoši teške pasmine hranjene uz dodatak prirodnog 1,25(OH)2D3-glikozida

master's thesis

• Title (english): ˝Differential blood cell count in broiler breeder hens fed with addition of 1,25(OH)2D3- glycoside˝
• Author: Ivan Lošić
• Mentor: Željko Gottstein (mentor)
• Mentor: Lana Pađen (mentor)
• Committee member: Jasna Aladrović (predsjednik povjerenstva)
• Committee member: Lana Pađen (član povjerenstva)
• Committee member: Željko Gottstein (član povjerenstva)
• Committee member: Marinko Vilić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2023-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Basic and Preclinical Veterinary Sciences

Abstract

Aktivni metabolit vitamina D 1,25(OH)2D3 ima mnoge važne uloge u organizmu ptica i
sisavaca. U intenzivnoj proizvodnji je važno pravovremeno praćenje zdravlja peradi, pri čemu
hematološke i biokemijske pretrage mogu biti od iznimnog značaja. Cilj ovog istraživanja bio
je utvrditi utjecaj dodavanja 1,25(OH)2D3-glikozida biljnog podrijetla u hranu na ukupan broj
i populacije leukocita te koncentracije kalcija, fosfora i magnezija u krvi. Istraživanje je
provedeno na 10000 nesilica kokoši teške linije Ross 308. Pokusnoj skupini nesilica u hranu je
umiješan 1,25(OH)2D3-glikozid biljnog podrijetla, dok je kontrolna skupina konzumirala
komercijalno dostupnu hranu, sukladno preporukama za genetiku. Uzorci venske krvi uzeti su
punkcijom krilne vene u epruvete s litijevim heparinom kao antikoagulansom i u epruvete s
gelom. Krv je uzorkovana 14, 25 i 35 dana od početka dodavanja pripravka vitamina D u hranu.
U uzorcima krvi određen je ukupan broj leukocita te su iz krvnog razmaza diferencirane
morfološki populacije leukocita. Izračunat je apsolutni broj pojedinih morfoloških oblika
leukocita te omjer heterofila i limfocita. U uzorcima seruma određene su koncentracije kalcija,
fosfora i magnezija. U ovom istraživanju dodavanje 1,25(OH)2D3-glikozida biljnog podrijetla
u hranu nesilica dovelo je do smanjenja ukupnog broja heterofila, limfocita i eozinofila uz
povećanje postotka limfocita, te povećanje koncentracije kalcija i magnezija u krvi. Na osnovi
rezultata možemo zaključiti kako suplementiranje nesilica 1,25(OH)2D3-glikozidom nakon 55.
tjedna starosti smanjuje omjer H/L ukazujući na manju osjetljivost na stres, te povećava
koncentraciju kalcija u serumu čime je omogućena proizvodnja kvalitetnije ljuske jaja.

Abstract (english)

The active metabolite of vitamin D, 1,25(OH)2D3, has many important roles in birds and
mammals. In intensive production, timely monitoring of poultry health is important, and
hematological and biochemical tests can be extremely important. The aim of this study was to
determine the effect of adding 1,25(OH)2D3-glycosides of plant origin to poultry feed on the
total number and population of leukocytes and the concentration of calcium, phosphorus and
magnesium in the blood. The study was conducted on 10,000 broiler breeder hens of the Ross
308 line. The experimental group of laying hens was fed with 1,25(OH)2D3-glycoside of plant
origin, while the control group consumed commercially available food, according to genetic
recommendations. Venous blood samples were taken by wing vein puncture in lithium heparin
anticoagulant and in gel tubes. Blood was taken 14, 25 and 35 days after the start of adding
vitamin D supplement into feed. The total number of leukocytes in the blood samples was
determined together with populations of leukocytes differentiated from the blood smear. The
absolute number of individual leukocytes’ populations and the ratio of heterophiles to
lymphocytes were calculated. Concentrations of calcium, phosphorus and magnesium were
determined in serum samples. In this study, the addition of 1,25(OH)2D3-glycosides of plant
origin to laying hens led to a decrease in the total number of heterophiles, lymphocytes and
eosinophils with an increase in the percentage of lymphocytes and an increase in blood calcium
and magnesium levels. Based on the results, we can conclude that supplementation of laying
hens with 1,25(OH)2D3-glycoside after 55 weeks of age temporarily reduces the H/L ratio
indicating lower sensitivity to stress, and increases serum calcium concentration, which allows
the production of better quality egg shells.

• Keywords: 1; 25(OH)2D3 -glikozid; nesilice; limfociti; heterofili; eozinofili; kalcij; fosfor; magnezij
• Keywords (english): 1; 25(OH)2D3-glycoside; laying hens; lymphocytes; heterophiles; eosinophils; calcium; phosphorus; magnesium
• Language: croatian
• URN:NBN: urn:nbn:hr:178:932160
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-07-19 09:00:04