Uporaba CD3 i CD79 protutijela u svrhu utvrđivanja omjera tumor-infiltrirajućih limfocita (TIL) kod planocelularnih karcinoma uške u mačaka

master's thesis

• Title (english): Use of CD3 and CD79 antibodies to determine the ratio of tumor-infiltrating lymphocytes (TIL) in squamous cell carcinomas of the auricle in cats
• Author: Nikola Serdar
• Mentor: Marko Hohšteter (mentor)
• Mentor: Lidija Medven Zagradišnik (mentor)
• Committee member: Ivan-Conrado Šoštarić-Zuckermann (predsjednik povjerenstva)
• Committee member: Lidija Medven Zagradišnik (član povjerenstva)
• Committee member: Marko Hohšteter (član povjerenstva)
• Committee member: Andrea Gudan Kurilj (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2023-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Planocelularni karcinom (engl. squamous cell carcinoma, SCC ) mačaka najučestalija je
kožna neoplazija koja se dominantno pojavljuje na području uške, nosnog planuma, koži kapka i
temporalnoj regiji. Najčešće zahvaća jedinke bijele boje u tropskim krajevima, s vršnom
incidencijom između 9. i 14. godine života. Istraživanje je provedeno na 30 arhivskih uzoraka SCC
uške mačaka koji su prikupljeni i arhivirani na Zavodu za veterinarsku patologiju Veterinarskog
fakulteta Sveučilišta u Zagrebu tijekom desetogodišnjeg razdoblja. Istraživanje je obuhvatilo
histopatološku i imunohistokemijsku (CD3 i CD79) pretragu navedenih uzoraka.
U istraživanju je promatrana korelacija između omjera CD3+ i CD79+ limfocita s pojedinim
stupnjem malignosti po Brodersovom, odnosno Bryneovom gradacijskom sustavu. Istraživanjem
je utvrđeno kako se SCC najčešće javlja u ženki (60%) križanih pasmina mačaka (40%) u
prosječnoj dobi od 9 godina. Tumorska nekroza i multifokalni limfocitno-plazmacitni infiltrat
zabilježen je u svim uzorcima (100%). Prosječan broj mitoza po vidnom polju velikog povećanje
iznosio je prosječno 3,5. Iako u istraživanju nije utvrđena statistički značajna korelacija između
TIL-a s pojedinim stupnjem malignosti, uočene su oscilacije prosječnog broja TIL-a s malignošću
tumora. S obzirom na sve veći značaj TIL-a kao prognostičkog čimbenika u suvremenoj medicini
potrebno je dodatno istražiti njihovu ulogu u svrhu poboljšanja dijagnostike, terapije i prognoze
bolesti.

Abstract (english)

Feline SCC is the most common skin neoplasm, occurring mainly on the ear, nasal planum,
eyelid skin and temporal region. White animals in tropical regions are most commonly affected,
with the highest incidence occurring between the ages of 9 and 14 years. The study was performed
on 30 archival samples of SCC on the auricle of cats collected and archived at the Department of
Veterinary Pathology, Faculty of Veterinary Medicine, University of Zagreb, over a ten-year
period. The study included histopathological and immunohistochemical (CD3 and CD79)
examination of the mentioned samples. The study investigated a correlation between the ratio of
CD3+ and CD79+ lymphocytes and a certain grade of malignancy according to the Broder's and
Bryne's grading system. Studie has showed that SCC occurs most frequently in female individuals
(60%) of mixed-breed cats (40%) at an average age of 9 years. Tumor necrosis and multifocal
lymphocytic-plasmacytic infiltrate were detected in all samples (100%). The average number of
mitoses per high-power field (HPF) was 3.5. Although no statistically significant correlation
between TILs and a specific malignancy grade was found in the study, variations in the average
number of TILs with tumor malignancy were observed. Given the increasing importance of TIL
as a prognostic factor in modern medicine, it is necessary to further investigate its role in order to
improve diagnosis, therapy, and prognosis of the disease.

• Keywords: planocelularni karcinom (SCC); tumor-infiltrirajući limfociti (TIL); CD3+; CD79+; mačka
• Keywords (english): squamous cell carcinoma (SCC); tumor-infiltrating lymphocytes (TIL); CD3+; CD79+; cat
• Language: croatian
• URN:NBN: urn:nbn:hr:178:346267
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-11-02 08:39:08