Poredbena analiza titra specifičnih protutijela u serumu i količine bakterije Mycoplasma synoviae u obrisku dušnika s ciljem poboljšanja biosigurnosnih mjera na farmama kokoši nesilica lake pasmine

master's thesis

• Title (english): Comparative analysis of specific antibody titres in serum and quantity of Mycoplasma synoviae bacteria in tracheal swabs with the aim of enhancing biosecurity measures on layer hen farms
• Author: Lucija Kurevija
• Mentor: Željko Gottstein (mentor)
• Mentor: Kristina Matković (mentor)
• Committee member: Maja Lukač (predsjednik povjerenstva)
• Committee member: Kristina Matković (član povjerenstva)
• Committee member: Željko Gottstein (član povjerenstva)
• Committee member: Mario Ostović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2024-03, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Public Health and Food Safety

Abstract

Bakterija Mycoplasma synoviae na farmama kokoši nesilica može uzrokovati pojavu bolesti
dišnog sustava, promjena na sinovijalnim membranama te abnormalnost apeksa jajne ljuske i
time znatno smanjiti proizvodnost jata i kvalitetu jaja, što može dovesti do velikih ekonomskih
gubitaka. Sprječavanje unosa i širenja uzročnika zasniva se na pravilnom provođenju
biosigurnosnih mjera. U tu je svrhu potrebno provoditi monitoring za koji najčešće koristimo
ELISA test i qPCR. Dokaz specifičnih protutijela ELISA testom služi kao dokaz prethodne
zaraze jata te je indikator za provođenje qPCR postupka, dok qPCR služi kao dokaz rane zaraze
jata. Provođenjem ovih metoda dobiva se uvid u kvalitetu provođenja biosigurnosnih mjera na
farmama. U ovom istraživanju utvrđivao se odnos specifičnih protutijela u serumu i broja kopija
genoma u obrisku dušnika. Analizom rezultata svih jata međusobno dobivena je visoka
pozitivna razina korelacije između titra specifičnih protutijela u serumu i broja kopija genoma
bakterije MS u obrisku dušnika. Na razini pojedinih jata statistički značajna pozitivna korelacija
prisutna je samo kod jednog jata, dok je kod drugih jata prisutna tendencija da je uz viši titar
prisutan veći broj, kao i uz niži titar manji broj kopija genoma bakterije MS. Viši titrevi su
dokazani u starijih jata, dok je kod mlađih jata obično utvrđen nizak titar i broj kopija genoma.
Izuzetak je jedno jato kod kojeg je uz visok titar prisutan negativan nalaz genoma, što može
ukazati na mogući propust pri uzorkovanju. Utvrđeni rezultati ukazuju na potrebu za
kontinuiranim monitoringom MS na farmama uz jačanje biosigurnosnih mjera i primjenu
cjepiva s ciljem istiskivanja divljih sojeva s farmi.

Abstract (english)

Bacteria Mycoplasma synoviae on layer hen farms can cause respiratory diseases, changes on
synovial membranes and abnormalities in the apex of the eggshell, and significantly reduce
flock productivity and egg quality, what can lead to significant economic losses. Preventing the
introduction and spread of the pathogen is based on proper implementation of biosecurity
measures. For this purpose, it is useful to conduct monitoring most often using ELISA tests and
qPCR. The evidence of specific antibodies via ELISA test serves as proof of previous flock
infection and as an indicator for conducting qPCR, while qPCR serves as evidence of early
flock infection. By implementing these methods, insight into the quality of biosecurity measures
on farms is obtained. This study aimed to establish the relationship between titre of specific
antibodies in serum and the number of genome copies of MS in tracheal swabs. Analysis of the
results from all flocks revealed a high level of positive correlation between them. On the flock
level significant positive correlation is present only in one flock, while in other flocks tendency
is present which shows that higher titres are followed by higher number of genome copies, and
vice versa. Usually higher titres are present in older, while lower titres in younger flocks.
Exception is present in one flock in which despite high ELISA titre no genome copies are
detected, what could be the error during the sampling. Achieved results confirm the need for
continuous monitoring of MS on layer farms with strengthening of biosecurity measures and
application of vaccines with the aim of elimination of wild strain from the farms.

• Keywords: Mycoplasma synoviae; monitoring; ELISA; qPCR; biosigurnosne mjere
• Keywords (english): Mycoplasma synoviae; monitoring; ELISA; qPCR; biosecurity measures
• Language: croatian
• URN:NBN: urn:nbn:hr:178:644917
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-03-13 12:59:57