Novi pristupi u vaginalnoj citologiji kuja

master's thesis

• Title (english): Bitch vaginal cytology – novel approaches
• Author: Antonia Čular
• Mentor: Nino Maćešić (mentor)
• Mentor: Ivan Butković (mentor)
• Committee member: Ivan Folnožić (predsjednik povjerenstva)
• Committee member: Nino Maćešić (član povjerenstva)
• Committee member: Ivan Butković (član povjerenstva)
• Committee member: Tugomir Karadjole (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2024-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Kuje su monoestrične životinje kod kojih se spolni ciklus sastoji od proestrusa, estrusa,
diestrusa i anestrusa. Pubertet se javlja u dobi od 6 do 23 mjeseca starosti, u prosjeku od 10 do
24 mjeseca starost. Spolni ciklus kuje se razlikuje od većine vrsta sisavaca zbog specifičnosti
da ovuliraju jajne stanice u nezrelom stanju koje nije moguće odmah oploditi. Primarnim jajnim
stanicama je potrebno još jedno dijeljenje i sazrijevanje prije uspješne oplodnje. Također,
specifičnost spolnog ciklusa kuje je i porast koncentracije progesterona uslijed luteinizacije
granuloza stanica jajnika prije ovulacije. Za određivanje optimalnog vremena parenja kuja
koristi se više metoda među kojima su najpoznatije vaginoskopski pregled, mjerenje
koncentracije progesterona i LH u serumu te vaginalna citologija. Vaginalna citologija je
jednostavna klinička metoda koja se sastoji od uzorkovanja, bojenja i mikroskopskog pregleda
odljuštenih vaginalnih epitelnih stanica. Vaginalni epitel zadeblja kao posljedica porasta
koncentracije estrogena u plazmi. Povećani broj staničnih slojeva vaginalnog epitela ima za
rezultat smanjenu opskrbu epitelnih stanica krvlju, njihovo odumiranje te konačno ljuštenje u
vaginalnu tekućinu. Postotak određenih tipova epitelnih stanica vidljive u uzorku nam daju
informacije o fazi spolnog ciklusa u kojem se kuja nalazi. Vaginalna citologija, uz praćenje
spolnog ciklusa, ima važnu ulogu u dijagnosticiranju raznih patoloških stanja reproduktivnog
sustava.

Abstract (english)

Bitches are monoestrous animals in which the reproductive cycle consists of proestrus, oestrus,
dioestrus and anoestrus. Puberty occurs between 6 and 23 months of age, on average between
10 and 24 months of age. The reproductive cycle of bitches differs from most mammalian
species because they ovulate immature oocytes that cannot be fertilised immediately. Primary
oocytes need one more division and maturation before successful fertilisation. Also, the canine
reproductive cycle is characterised by an increase in the concentration of progesterone due to
the luteinisation of the granulosa cells of the ovary before ovulation. Several methods are used
to determine the optimal mating time of bitches, among which the most famous are
vaginoscopy, measurement of progesterone and LH serum concentrations, and vaginal
cytology. Vaginal cytology is a simple clinical method consisting of sampling, staining and
microscopic examination of exfoliated vaginal epithelial cells. The vaginal epithelium thickens
as a result of increased concentrations of oestrogen in the plasma. The increased number of cell
layers of the vaginal epithelium results in a reduced supply of epithelial cells with blood, their
death and eventual shedding into the vaginal fluid. The percentage of certain types of epithelial
cells visible in the sample gives us information about the phase of the reproductive cycle of the
bitch. Vaginal cytology, along with monitoring the reproductive cycle, plays an important role
in the diagnosis of various pathological conditions of the reproductive system.

• Keywords: kuja; spolni ciklus; vaginalna citologija; epitelne stanice
• Keywords (english): bitch; reproductive cycle; vaginal cytology; epithelial cells
• Language: croatian
• URN:NBN: urn:nbn:hr:178:839180
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-01-10
• Created on: 2024-06-06 12:49:58