Vrste i antimikrobna osjetljivost beta-hemolitičkih streptokoka izdvojenih iz konja

master's thesis

• Title (english): Species and antimicrobial susceptibility of beta-hemolytic streptococci isolated from horses
• Author: Klara Fuš
• Mentor: Zrinka Štritof (mentor)
• Committee member: Matko Perharić (predsjednik povjerenstva)
• Committee member: Suzana Hađina (član povjerenstva)
• Committee member: Zrinka Štritof (član povjerenstva)
• Committee member: Vilim Starešina (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2024-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Public Health and Health Care; Public Health

Abstract

Bakterije roda Streptococcus česti su uzročnici infekcija različitih organskih sustava kod
konja. Najvažnije vrste streptokoka u konja, Streptococcus equi subsp. equi (S. equi),
Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) i Streptococcus dysgalactiae
subsp. equisimilis (S. equisimilis), tvore beta-hemolizu na krvnom agaru zbog čega pripadaju
skupini beta-hemolitičkih streptokoka (BHS). Cilj ovog istraživanja bio je istražiti učestalost
izdvajanja BHS-a iz kliničkih uzoraka konja, raspodjelu vrsta i njihovu antimikrobnu
osjetljivost u razdoblju od 2013. do 2022. godine. U devetogodišnjem razdoblju, iz 803 klinička
uzorka izdvojeno je 96 izolata beta-hemolitičkih streptokoka, od kojih su 73 izolata pohranjena
za daljnju analizu i uključena u ovo istraživanje. Najveći broj izolata BHS-a (50,7%) bio je
porijeklom iz respiratornog sustava. Vrste BHS-a bile su identificirane pomoću komercijalnih
biokemijskih testova (API) i MALDI-TOF MS metode. Najčešće vrste BHS-a bile su S.
zooepidemicus (72,6%) i S. equisimilis (19,2%). Većina izolata vrste S. zooepidemicus (55%)
bila je porijeklom iz respiratornog sustava kao i polovica izolata S. equisimilis (50%). Svi izolati
BHS bili su osjetljivi na penicilin i ceftiofur. Na trimetoprim/sulfametoksazol bilo osjetljivo
96,2% izolata, a na enrofloksacin 73,6% izolata S. zooepidemicus. Na
trimetoprim/sulfametoksazol i eritromicin bilo je osjetljivo 92,9%, a na enrofloksacin samo
7,1% izolata S. equisimilis. Rezultati ovog istraživanja potvrđuju važnost BHS-a kao uzročnika
infekcija kod konja te ističu potrebu za daljnjim praćenjem antimikrobne rezistencije

Abstract (english)

Bacteria of the genus Streptococcus are common causative agents of infections in
various organ systems of horses. The most important streptococcal species in horses,
Streptococcus equi subsp. equi (S. equi), Streptococcus equi subsp. zooepidemicus (S.
zooepidemicus), and Streptococcus dysgalactiae subsp. equisimilis (S. equisimilis), produce
beta-hemolysis on blood agar, and therefore belong to the group of beta-hemolytic streptococci
(BHS). The aim of this study was to investigate the occurrence of BHS isolated from clinical
samples of horses, species distribution, and their antimicrobial susceptibility over the period
from 2013 to 2022. Over the nine-year period, 96 isolates of beta-hemolytic streptococci were
isolated from 803 clinical samples, of which 73 isolates were preserved for further analysis and
included in this study. The majority of BHS isolates (50.7%) originated from the respiratory
system. BHS species were identified using commercial biochemical tests (API) and the
MALDI-TOF MS method. The most common BHS species were S. zooepidemicus (72.6%) and
S. equisimilis (19.2%). Most of the S. zooepidemicus isolates (55%) were from the respiratory
system, as were half of the S. equisimilis isolates (50%). All BHS isolates were susceptible to
penicillin and ceftiofur. Majority (96.2%) of the S. zooepidemicus isolates were susceptible to
trimethoprim/sulfamethoxazole, and 73.6% to enrofloxacin. Majority (92.9%) of the S.
equisimilis isolates were susceptible to trimethoprim/sulfamethoxazole and erythromycin, but
only 7.1% were susceptible to enrofloxacin. The results of this study confirm the importance of
BHS as causative agents of infections in horses and highlight the need for continued monitoring
of antimicrobial resistance.

• Keywords: beta-hemolitički streptokoki; konji; S. zooepidemicus; antimikrobna osjetljivost
• Keywords (english): beta-hemolytic streptococci; horses; S. zooepidemicus; antimicrobial susceptibility
• Language: croatian
• URN:NBN: urn:nbn:hr:178:192819
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-21 11:14:02