Usporedba manualnih i automatskih analiza krvne slike u veterinarskoj medicini

master's thesis

• Author: Ilija Vrbanić
• Mentor: Ana Shek Vugrovečki (mentor)
• Committee member: Maja Belić (predsjednik povjerenstva)
• Committee member: Ivona Žura Žaja (član povjerenstva)
• Committee member: Ana Shek Vugrovečki (član povjerenstva)
• Committee member: Lana Pađen (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2024, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Basic and Preclinical Veterinary Sciences

Abstract

Rad se bavi usporedbom manualnih i automatskih analiza krvne slike u veterinarskoj medicini, istražujući prednosti i nedostatke svake metode. Manualne metode, poput mikroskopske evaluacije krvnih razmaza, i dalje igraju ključnu ulogu u veterinarskoj praksi, posebice u prepoznavanju suptilnih morfoloških promjena koje automatski analizatori ponekad ne mogu otkriti. Unatoč tome, manualne metode zahtijevaju visoku razinu stručnosti i vremenski su zahtjevne, što može ograničiti njihov kapacitet u laboratorijima s velikim brojem uzoraka. S druge strane, automatski analizatori predstavljaju tehnološki napredak koji omogućava bržu, precizniju i učinkovitiju analizu. Ovi uređaji mogu analizirati stotine uzoraka u kratkom vremenu, smanjujući mogućnost ljudske pogreške i pružajući konzistentne rezultate. Automatski analizatori koriste napredne tehnologije kao što su laserska difrakcija, protočna citometrija i električna impedancija za analizu ključnih krvnih parametara, što ih čini nezaobilaznim alatima u suvremenoj veterinarskoj dijagnostici. U radu se kao primjer manualne analize spominje jazavčar, pasmina koja često pati od specifičnih hematoloških poremećaja. U slučaju psa zaraženog Mycoplasma haemocanis, manualna analiza krvnog razmaza omogućila je identifikaciju patogena, što automatski analizatori nisu mogli precizno detektirati. Ovaj primjer naglašava važnost ručne procjene u složenim slučajevima, osobito kada su prisutne infekcije ili morfološke promjene koje zahtijevaju detaljan pregled. Zaključno, iako automatski analizatori donose brzinu i standardizaciju u analizi krvne slike, manualne metode ostaju nezamjenjive u složenim dijagnostičkim postupcima. Kombinacija obje metode omogućuje optimalne rezultate, povećavajući točnost i pouzdanost dijagnostike u veterinarskoj medicini.

Abstract (english)

This paper compares manual and automated blood count analyses in veterinary medicine, examining the advantages and disadvantages of each method. Manual methods, such as microscopic evaluation of blood smears, continue to play a crucial role in veterinary practice, especially in identifying subtle morphological changes that automated analyzers may not detect. However, manual methods require a high level of expertise and are time-consuming, which can limit their capacity in laboratories with a large number of samples. On the other hand, automated analyzers represent a technological advancement that enables faster, more accurate, and efficient analysis. These devices can process hundreds of samples in a short time, reducing the possibility of human error and providing consistent results. Automated analyzers use advanced technologies such as laser diffraction, flow cytometry, and electrical impedance to analyze key blood parameters, making them indispensable tools in modern veterinary diagnostics. The paper includes the example of a dachshund as part of the manual analysis. Dachshunds, a breed prone to specific hematological disorders, are featured in a case of a dog infected with Mycoplasma haemocanis. Manual blood smear analysis allowed the identification of the pathogen, which automated analyzers could not accurately detect. This example highlights the importance of manual assessment in complex cases, especially when infections or morphological changes require a detailed examination. In conclusion, while automated analyzers bring speed and standardization to blood count analysis, manual methods remain indispensable in complex diagnostic procedures. The combination of both methods provides optimal results, increasing diagnostic accuracy and reliability in veterinary medicine.

• Keywords: manualna analiza krvne slike; automatska analiza krvne slike; usporedba; veterinarska medicina; dijagnostika
• Keywords (english): manual blood count analyses; automated blood count analyses; veterinary medicine; diagnostics
• Language: croatian
• URN:NBN: urn:nbn:hr:178:780234
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2024-10-24 07:18:09