ODREĐIVANJE PROŠIRENOSTI INFEKCIJA VIRUSOM KRPELJNOG MENINGOENCEFALITISA U KONJA NA PODRUČJU REPUBLIKE HRVATSKE

master's thesis

• Title (english): DETERMINATION OF THE PREVALENCE OF INFECTION WITH TICK-BORNE ENCEPHALITIS IN HORSES IN CROATIA
• Author: Marko Erlač
• Mentor: Ljubo Barbić (mentor)
• Committee member: Zoran Milas
• Committee member: Josip Madić
• Committee member: Ljubo Barbić
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Microbiology and Infectious Diseases with Clinic); Zagreb
• Defense date and country: 2016, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Virus krpeljnog meningoencefalitisa je arbovirus koji pripada porodici Flaviviridae, rodu Flavivirus, serokompleksu krpeljnog encefalitisa. Uzrokuje bolest koja se manifestira u obliku blage groznice, ali može uzrokovati ozbiljne neurološke poremećaje sa mogućim smrtnim ishodom ili trajnim posljedicama. Krpeljni meningoencefalitis se pojavljuje enzootski i sezonski u Republici Hrvatskoj, međutim organizirani sustav nadziranja vKME nije uspostavljen, a istraživanja na životinjama su rijetko provođena. Cilj ovog istraživanja bio je istražiti pojavnost infekcije konja u Hrvatskoj u diferencijalno dijagnostičke svrhe, ali i usporediti rezultate seroprevalencije u konja sa stopom incidencije u ljudi kako bi ustanovili mogu li podaci prikupljani veterinarskim nadzorom imati značaja za javno zdravstvo.
Za istraživanje su nasumice odabrani uzorci seruma zdravih konja iz arhive ARTERlab s područja 6 različitih županija. Županije su odabrane na temelju incidencije oboljenja u ljudi. Za serološku dijagnostiku KME korišten je komercijalni dijagnostički komplet „Immunozym FSME IgG All species“ (Progen Biotechnik, Heidelberg, Njemačka). Podaci o incidenciji oboljenja u ljudi prikupljeni su iz arhive Hrvatskog zavoda za javno zdravstvo.
Najviša seroprevalencija je u Međimurskoj županiji s 23,6% pozitivna i 7,9% granična rezultata. Sisačko-moslavačka ima 4,5% pozitivna i 3,4% granična rezultata. Karlovačka županija ima 4,5% pozitivna te 1,1% granična rezultata. Grad Zagreb nema pozitivne, ali ima 10,1% graničnih rezultata. Brodsko-posavska županija također nema pozitivnih, ali ima 2,2% granična rezultata. Istarska županija nisu zabilježeni niti pozitivni niti granični rezultati. Rezultati ovog istraživanja s dokazom visoke seroprevalencije indiciraju da KME također treba uzeti u obzir u diferencijalnoj dijagnostici neuroloških bolesti u konja na području RH.
Analiza podataka iz arhiva Hrvatskog zavoda za javno zdravstvo te rezultata dobivenih ovim istraživanjem pokazala je korelaciju između seroprevalencije u konja i incidencije oboljenja ljudi što potvrđuje da se konji mogu koristiti kao sentinel životinje za nadzor KME te da bi veterinarski nadzor ove zoonoze mogao značajno doprinositi javnom zdravstvu.

Abstract (english)

Tick-borne encephalitis virus is an arbovirus belonging to the Flaviviridae family, flavivirus genus, tick-borne encephalitis serocomplex. It causes a disease that manifests itself in the form of mild fever, but it can cause serious neurological disorders with possible fatal or lasting consequences. Tick-borne encephalitis occurs enzootic and seasonally in Croatia, however, an organized system of monitoring TBEV has not been established, and animal studies are rarely carried out. The aim of this study was to investigate the prevalence of infection in horses for differential diagnostic purposes, but also to compare the results of seroprevalence in horses with the rate of incidence in humans to determine whether the data collected via veterinary supervision have significance for public health.
For the purpose of this study, randomly selected sera samples from healthy horses from six different counties were chosen from the ARTERlab archives. The counties were selected based on human incidence. The commercial diagnostic kit "Immunozym TBE IgG All species" (Progen Biotechnik, Heidelberg, Germany) was used for serological diagnosing of TBE. Data on the incidence in humans was collected from the archives of the Croatian Institute for Public Health.
The highest seroprevalence was in Međimurje county with 23.6% positive and 7.9% borderline results. Sisak-Moslavina has a positive 4.5% and 3.4% borderline results. Karlovac has 4.5% positive and 1.1% borderline results. Zagreb has no positive, but has 10.1% borderline results. Brod-Posavina also has no positive, but has a 2.2% borderline results. Istria has no positive or borderline results.
The results of this research are evidence of high seroprevalence and indicate that TBE should also be considered in the differential diagnosis of neurological diseases in horses in Croatia.
Analysis of data from the archives of the Croatian Institute for Public Health and the results obtained in this study shows a correlation between seroprevalence in horses and human incidence, which confirms that horses can be used as sentinel animals for monitoring TBE and that veterinary supervision of this zoonoses could significantly contribute to public health .

• Keywords: virus krpeljnog meningoencefalitisa; konji; ELISA; zoonoza; program nadzora; Republika Hrvatska
• Keywords (english): tick-borne encephalitis virus; horses; zoonoses; ELISA; surveillance; Croatia
• Language: croatian
• URN:NBN: urn:nbn:hr:178:785823
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-02-16 13:38:19