Histopathological changes in pumpkinseed (Lepomis gibbosus) naturally infected with Schulmanela petruschewskii (Nematoda: Capillariidae)

master's thesis

• Title (croatian): Histopatološke promjene u sunčanice (Lepomis gibbosus) invadirane oblićem Schulmanela petruschewskii (Nematoda: Capillariidae)
• Author: Sofie Elisabeth Sörhannus
• Mentor: Emil Gjurčević (mentor)
• Mentor: Krešimir Matanović (mentor)
• Committee member: Krešimir Severin (predsjednik povjerenstva)
• Committee member: Franjo Martinković (član povjerenstva)
• Committee member: Emil Gjurčević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2024-11-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Animal Production and Biotechnology

Abstract

This study provides data on the parasite fauna of pumpkinseed (Lepomis gibbosus) from
Croatian waters and describes the pathological effect of the nematode S. petruschewskii in this
species of fish. Detailed parasitological examination of the fish revealed an ancyrocephalid
monogenean Onchocleidus sp., metacercariae of Posthodiplostomum sp., myxosporean
Myxobolus sp. and the nematode S. petruschewskii. The pathological effect of S. petruschewskii
was evaluated by histopathology. For histological examination, liver sections were stained with
haematoxylin and eosin and periodic acid-Schiff. Microscopically, infection with S.
petruschewskii causes a mild to moderate multifocal granulomatous response and could
represent a space-occupying threat to normal liver function. The observed granulomas are
mainly composed of epithelioid cells and macrophage aggregates. Rarely contain a connective
tissue capsule. When present in large numbers, the adults and eggs of S. petruschewskii are
capable of inducing severe liver injury and ultimately causing fish mortality.

Abstract (croatian)

U diplomskom radu prikazana je parazitofauna sunčanice (Lepomis gibbosus) porijeklom iz
otvorenih voda Republike Hrvatske i opisane su patološke promjene nastale kao posljedica
invazije oblićem S. petruschewskii. Parazitološkom pretragom u prikupljenih primjeraka
utvrđena je pisutnost jednorodnog metilja Onchocleidus sp., dvodomnog metilja
Posthodiplostomum sp., nametnika Myxobolus sp. i oblića S. petruschewskii. Patološki učinak
invazije oblićem S. petruschewskii procijenjen je histološkom pretragom. U svrhu histološke
pretrage, uzorci jetre invadiranih riba bojani su hematoksilin-eozinom i periodičnom kiselinom
Schiff. Kao posljedica invazije oblićem S. petruschewskii dolazi do granulomatozne reakcije u
jetri, a utvrđeni granulomi sastavljeni su od epiteloidnih stanica i nakupina makrofaga, s ili bez
vezivnotkivne kapsule. Jake invazije uzrokuju gubitak parenhima i smanjenje jetrene funkcije
što u konačnici može dovesti do uginuća ribe.

• Keywords: pumpkinseed; Schulmanela petruschewskii; histopathology
• Keywords (croatian): sunčanica; Schulmanela petruschewskii; histopatologija
• Language: english
• URN:NBN: urn:nbn:hr:178:817620
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-11-25
• Created on: 2025-01-07 14:34:41