| Abstract | Chronic enteropathies in dogs refer to gastrointestinal symptoms persisting for more than 3
weeks, excluding diseases of other organ systems, neoplasia, and infectious agents. Clinically,
chronic enteropathy syndrome is categorized according to response to treatment:
hypoallergenic food, antibiotic therapy, and immunosuppressive therapy. Previous studies have
shown that more than 50% of dogs respond to treatment that includes a highly digestible
hypoallergenic diet with stabilization of symptoms. One critical factor in the pathogenesis of
chronic enteropathy is oxidative stress, which contributes to the underlying inflammation and
tissue damage in the gastrointestinal tract. Given its role in disease progression, improving
oxidative status may be key to alleviating symptoms in affected dogs. Chestnut tannins, a group
of phytochemicals with antioxidant, anti-inflammatory, antiviral and other therapeutic
properties, have been suggested as a potential supplement to address this imbalance. Therefore,
this study aimed to evaluate the effects of a hypoallergenic home-made diet with a novel protein
source and a hypoallergenic diet with hydrolyzed proteins, supplemented with chestnut tannins,
on the antioxidant status of dogs diagnosed with chronic enteropathy. 8 dogs with chronic
enteropathy were included in the study. Blood was collected twice, before and after an
elimination diet and tannin supplementation. Plasma MDA levels were measured by the HPLC
method on a Shimadzu liquid chromatograph using an InertSustain C-18 column and a UV/Vis
detector, while the activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD)
was measured using commercial kits (Ransel and Ransod, respectivley) on an automated
biochemical analyzer. Despite no significant changes in the oxidative stress markers, clinical
improvement was observed, suggesting effects of tannins beyond their antioxidant properties.
Further studies with larger sample size, extended supplementation duration and dose
optimization are needed to better understand the impact of tannins on oxidative stress markers
and their therapeutic effects in dogs with chronic enteropathy. |
| Abstract (croatian) | Kronične enteropatije kod pasa pojam je koji opisuje prisutnost gastrointestinalnih simptoma u
trajanju duljem od 3 tjedna pri čemu su isključene bolesti ostalih organskih sustava, neoplazije
te ostali infektivni uzročnici. Klinički, sindrom kroničnih enteropatija se dijeli na temelju
odgovora na tretman: hipoalergena hrana, antibiotska terapija i terapija imunosupresivima. U
prethodno provedenim istraživanjima utvrđeno je da će na tretman koji uključuje hipoalergeni
obrok visoke probavljivosti odgovoriti više od 50% pasa u vidu stabilizacije simptoma. Jedan
od ključnih faktora koji utječe na patogenezu kronične enteropatije je i oksidativni stres koji
potiče upalne procese čime se narušava integritet probavnog sustava. Stoga će intervencije koje
za cilj imaju pozitivan učinak na antioksidativni status, pozitivno djelovati na progresiju bolesti
pasa sa kroničnim eneropatijama. Tanini kestena čine opsežnu grupu botaničkih spojeva koji
posjeduju antimikrobno, protuupalno, antiviralno te antidiaroično djelovanje. Stoga je cilj
istraživanja bio ustanoviti učinak pripravaka kestenovog tanina uz eliminacijski protokol
hranjenja na antioksidativni status pasa sa kroničnim enteropatijama. 8 pasa sa dijagnozom
kronične enteropatije uključeno je u istraživanje. Analiza MDA u serumu utvrđena je HPLC
metodom na tekućinskom kromatografu Shimadzu pomoću InertSustain C-18 kolone i UV/Vis
detektora. Analiza SOD i GPx utvrđena je putem komercijalnih kitova postupkom ELISA-e
(Ransel i Ransod). Istraživanjem nisu utvrđene značajne razlike u kontrolnoj i pokusnoj skupini
na razini markera oksidativnog (MDA) i antioksidativnog statusa (SOD i GPx) ukazujući na
pozitivan učinak tanina pored antioksidativnih svojstava. Provedba dugoročnijih istraživanja na
većem broju pas te optimizacija doze i trajanja suplementacije pomoći će u boljem
razumijevanju učinaka tanina na markere oksidativnog stresa i njihov potencijalni terapeutski
učinak kod pasa sa kroničnim enteropatijama. |
