Usporedba dijagnostičkih postupaka u pretrazi izmeta pasa na prisutnost cisti Giardia duodenalis

master's thesis

• Title (english): Comparison of Diagnostic Procedures in the Search of Dog Feces for the Presence of Giardia duodenalis cysts
• Author: Romana Tikvić
• Mentor: Albert Marinculić (mentor)
• Committee member: Tatjana Živičnjak (predsjednik povjerenstva)
• Committee member: Dean Konjević (član povjerenstva)
• Committee member: Albert Marinculić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2025-01-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Public Health and Food Safety

Abstract

U ovom istraživanju uspoređeni su rezultati tri dijagnostička testa za giardiozu pasa
pretraživanjem izmeta. Uzorci izmeta prikupljeni su od 78 uzoraka pasa. Uzorci su od svakog
pas prikupljeni u tri navrata. Uzorci su sakupljeni neovisno o dobi, spolu i pasmini psa. Nakon
izvršene pretrage provedena je statistička analiza. S obzirom da je metoda imunofluorescencije
prihvaćena kao zlatni standard u dijagnostici giardioze pasa, u ovome radu su s navedenim
testom uspoređeni nalazi dobiveni metodom nativne flotacije i metodom flotacije uz bojenje
Lugolovom otopinom. Vrijednosti dobivene analizom podataka dobivenih iz ukupnog uzorka,
uzorka prema spolu te uzorka prema dobi, raspodijeljene su kao stvarno pozitivne (TP), stvarno
negativne (TN), lažno pozitivne (FP) i lažno negativne (FN). Izračunata je osjetljivost i
specifičnost svakog testa. Pored toga rezultati flotacije i flotacije uz bojenje Lugolovom
otopinom uspoređeni su međusobno pomoću Cohen Kappa testa. Izračunat je i Youdenov
indeks. Usporedbom rezultata flotacije i flotacije sa bojenjem s rezultatima testa izravnom
imunofluorescencijom utvrđeno je da imaju izrazito visoku specifičnost (100%), dok je
osjetljivost niža i iznosi 70,6% za test flotacije s bojenjem Lugolovom otopinom, odnosno
58,8% za test flotacije. Utvrđena specifičnost izravne imunofluorescencije od 100% ujedno
ukazuje na činjenicu da će testom sve negativne jedinke biti otkrivene, ali zbog pojave većeg
udjela lažno negativnih jedinki, oba testa daju veću dijagnostičku sigurnost u pozitivan nalaz
testa. Kako je i očekivano, metoda flotacije uz bojenje Lugolovom otopinom je nešto
pouzdanija metoda samim time što je vizualizacija parazita lakša, te uz visoku specifičnost daje
i nešto višu osjetljivost (70,6% vs. 58.8%) u odnosu na metodu flotacije bez bojenja.

Abstract (english)

In this study, the results of three diagnostic tests for canine giardiasis by fecal examination were
compared. Fecal samples were collected from 78 dog samples. Samples were collected from
each dog on three occasions. Samples were collected regardless of age, sex and breed of dog.
After the examination, a statistical analysis was performed. Since the immunofluorescence
method is accepted as the gold standard in the diagnosis of canine giardiasis, in this study, the
findings obtained by the native flotation method and the flotation method with Lugol's solution
staining were compared with the aforementioned test. The values obtained by analyzing the
data obtained from the total sample, sample by sex and sample by age were distributed as true
positive (TP), true negative (TN), false positive (FP) and false negative (FN). The sensitivity
and specificity of each test were calculated. In addition, the results of flotation and flotation
with Lugol's solution staining were compared with each other using the Cohen Kappa test. The
Youden index was calculated. Comparing the results of flotation and flotation with staining
with the results of the direct immunofluorescence test, it was found that they have an extremely
high specificity (100%), while the sensitivity is lower (70.6%) for the flotation test with Lugol's
solution staining and 58.8% for the flotation test. The determined specificity of direct
immunofluorescence (100%) indicates that all negative individuals will be detected by the test,
but due to the occurrence of a higher proportion of false-negative individuals, both tests provide
greater diagnostic certainty in a positive test result. As expected, the flotation method with
Lugol's solution staining is a slightly more reliable method simply because visualization of the
parasite is easier, and in addition to high specificity, it also provides a slightly higher sensitivity
(70.6% vs. 58.8%) compared to the flotation method without staining.

• Keywords: giardioza; izmet; imunofluorescencija; specifičnost; osjetljivost
• Keywords (english): giardiasis; feces; immunofluorescence; specificity; sensitivity
• Language: croatian
• URN:NBN: urn:nbn:hr:178:239311
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-01-22 09:54:50