Sinkronizacija junica za davateljice goveđih jajnih stanica u postupku oplodnje in vitro

master's thesis

• Title (english): Synchronization of Donor Heifers for Fertilization and Embryo Culture in vitro
• Author: Marko Adrić
• Mentor: Iva Getz (mentor)
• Committee member: Marko Samardžija
• Committee member: Juraj Grizelj
• Committee member: Iva Getz
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Reproduction and Obstetrics Clinic); Zagreb
• Defense date and country: 2014-04-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Svrha ovog istraživanja bila je usporediti utjecaj različitih metoda sinkronizacije estrusa na razvojnu sposobnost jajnih stanica i uzgoj in vitro goveđih zametaka. Za davateljice jajnih stanica odabrane su junice u ciklusu simentalske pasmine (n=12), koje su potom nasumice podijeljene u dvije skupine. Prva skupina junica sinkronizirana je s pomoću 2 injekcije PGF2 u razmaku od 11 dana. Druga skupina junica sinkronizirana je s pomoću gestagenskih ušnih umetaka tijekom 10 dana, te PGF2 injekcije 48 sati prije vađenja umetka. Kod svih je davateljica aktivnost jajnika stimulirana s pomoću pFSH, počevši od 9/10 dana ciklusa. Transvaginalna aspiracija jajnih stanica izvedena je 48 sati nakon zadnje injekcije pFSH. Zabilježen je broj aspiriranih folikula, broj aspiriranih jajnih stanica i uspjeh aspiracije. Jajne stanice dobre kvalitete (G1, G2 i G3) uzete su u postupak dozrijevanja, oplodnje i uzgoja in vitro.
Prosječni borj folikula, aspiriranih jajnih stanica, te jajnih stanica koje su ušle u IVP između ove dvije skupine junica nije se statistički razlikovao s obzirom na odabir metode sinkronizacije estrusa. Prosječni broj jajnih stanica loše kvalitete (G) pokazivao je tendenciju prema značajnosti (P 0,10) u grupi junica sinkroniziranih gestagenima. Postotak brazdanih jajnih stanica 2. dana uzgoja, morula i blastocista 7. dana uzgoja te postotak izlegnutih blastocista 9. dana nije se statistički razlikovao između pojedinih skupina. Postupak sinkronizacije
spolnog ciklusa nije utjecao na broj folikula i aspiriranih jajnih stanica, niti na rezultate uzgoja goveđih zametaka in vitro.

Abstract (english)

The aim of the study was to compare the effect of different synchronization protocols on oocyte retrieval and blastocyst production in vitro. Twelve Simmental heifers were randomly allocated to one of two synchronization groups. In the 1st group heifers were synchronized with two PGF2 injections in 11-days interval. In the 2nd group a progestin ear implant was inserted for 10 days and PGF2 was injected 48 hours befor implant removal. The same stimulation protocol with pFSH, starting on Day 9/10 of cycle, was used in all treatment groups. Ovum pick-up (OPU) was performed 48 hours after the last FSH injection. The number of aspirated follicles, number and quality of retrieved oocytes were recorded. Good quality oocytes (G1, G2 i G3) were matured, fertilized and cultured in vitro in SOFaaBSA till the Day 9.
The mean number of follicles and oocytes total number, as the numer of oocates used for IVP did not differ among synchronization groups. However, mean number of low quality (G4) oocytes showed a tendency toward significance (P 0.10) in progestin treatment group. No significant differences between treatment groups were observed in terms of cleavage rate on Day 2, morula/blastocyst rate on Day 7 and hatched blastocysts rate on Day 9. The results indicated that synchronization protocol did not affect the number of follicles and the recovered oocytes, or the number of in vitro produced embryos.

• Keywords: junica; sinkronizacija; jajna stanica; uzgoj in vitro
• Keywords (english): heifer; synchronization; oocyte; in vitro culture
• Language: croatian
• URN:NBN: urn:nbn:hr:178:012262
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2015-12-04 12:26:23