Oksidacijska stabilnost seruma konja tijekom priprema i alkarskog natjecanja

master's thesis

• Title (english): Oxidative Stability of Horse Serum during Training and Alka Competition
• Author: Lucija Iličić
• Mentor: Jasna Aladrović (mentor)
• Committee member: Lada Radin (predsjednik povjerenstva)
• Committee member: Jasna Aladrović (član povjerenstva)
• Committee member: Blanka Beer Ljubić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Physiology and Radiobiology); Zagreb
• Defense date and country: 2016-05-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Fizička aktivnost i vježbanje praćeno je pojačanom potrošnjom kisika i povećanim stvaranjem reaktivnih kisikovih spojeva što dovodi do pojave oksidacijskog stresa. Stres izazvan vježbanjem dovodi do oštećenja mišićnih stanica kao posljedice oksidacije makromolekula stanične membrane i membrana organela u citoplazmi. Cilj rada je bio istražiti kako priprema konja tri mjeseca prije viteške igre Alke uvodi životinju u spomenuto okruženje te utječe na aktivnosti antioksidativnih enzima, na aktivnost enzima kojima se prati stanje mišićnih stanica, te na koncentracije metaboličkih pokazatelja u serumu konja engleskog punokrvnjaka i hrvatskog toplokrvnjaka. Istraživanje je provedeno na 7 konja pasmine engleski punokrvnjak i 6 konja pasmine hrvatski toplokrvnjak. Uzorci krvi uzeti su konjima 30, 21 i 14 dana prije Alke, neposredno nakon natjecanja i 7 dana nakon Alke. U serumu je određena aktivnost GSH-Px, SOD, PON 1, CK, LDH, AST te koncentracije glukoze, triglicerida, NEFA, ukupnog kolesterola i HDL-kolesterola. Aktivnost SOD u serumu engleskog punokrvnjaka bila je značajno viša 21 dan prije Alke u odnosu na aktivnost dobivenu kontrolnim vađenjem. Koncentracije NEFA u serumu engleskih punokrvnjaka bile su značajno veće 30 dan prije Alke i nakon istrčane Alke u odnosu na koncenteracije dobivene u kontrolnom vađenju. U serumu hrvatskog toplokrvnjaka utvrđene su značajno veće aktivnosti AST i CK 30 dana prije Alke te aktivnost CK i koncentracije triglicerida nakon promjene režima treninga u odnosu na vrijednosti dobivene u serumu engleskog punokrvnjaka. Dobiveni rezultati upućuju na oksidativnu stabilnost seruma konja i pasminske razlike tijekom treninga i natjecanja.

Abstract (english)

Physical activity and exercise increase oxygen uptake and ROS generation which leads to oxidative stress. Exercise-induced stress induces oxidative damage of muscle cell membrane and organelles. The aims of this study were to investigate the effects of training, Alka competition and resting on antioxidative enzymes activity, activity of muscle cell enzymes and concentrations of metabolic parameters in blood serum of Thoroughbred horses and Croatian Warmblood horses. The experiment was carried out on 7 Thoroughbred horses and 6 Croatian Warmblood. Blood samples were collected 30, 21 and 14 days before Alka, after competition and 7 days after Alka. Serum activities of GSH-Px, SOD, PON 1, CK, LDH, AST and concentrations of glucose, triacylglycerols, nonesterified fatty acids (NEFA), total cholesterol, high density lipoprotein cholesterol (HDL-C) were determined. SOD activity in Thoroughbred horses serum was significantly higher 21 days before Alka compared to activity in control samples. NEFA concentration in Thoroughbred horses serum were significantly higher 30 days before Alka and after the race compared to concentrations in control samples. In Croatian Warmblood horses’ blood serum activity of AST and CK were significantly higher 30 days before Alka, as well as triacylglycerols concentrations 21 days before competition compared to concentrations in Thoroughbred horses. The results indicate oxidative stability of horse serum and breed differences during training and competition.

• Keywords: konji; vježbanje; antioksidativni enzimi; metabolički profil
• Keywords (english): horses; exercise; antioxidative enzymes; metabolic profile
• Language: croatian
• URN:NBN: urn:nbn:hr:178:412369
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Reformatted digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-07-12 10:28:47