Utjecaj vremena ekvilibracije u postupku dubokog smrzavanja bičjeg sjemena

master's thesis

• Title (english): The Effect of Equilibration Time on Cryopreservation of Bovine Semen
• Author: Igor Nazansky
• Mentor: Marko Samardžija (mentor)
• Mentor: Martina Lojkić (mentor)
• Committee member: Silvijo Vince (predsjednik povjerenstva)
• Committee member: Martina Lojkić (član povjerenstva)
• Committee member: Marko Samardžija (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Reproduction and Obstetrics Clinic); Zagreb
• Defense date and country: 2018-05-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Cilj ovog istraživanja bio je istražiti utjecaj različitog vremena ekvilibracije u postupku dubokog smrzavanja bičjeg sjemena (DSS). U tu svrhu korišteni su ejakulati od 10 bikova koji se koriste u redovitoj proizvodnji duboko smrznutog bičjeg sjemena. Istraživanje je provedeno na 60 ejakulata (6 ejakulata po biku). Kako bi ustanovili učinak različitog vremena ekvilibracije na kvalitetu sjemena nakon otapanja, svaki dan prilikom kojeg je dobivan ejakulat uključivao je različite periode ekvilibracije i to: 3 sata (kontrola), 24 sata i 48 sati. Kvalitativne karakteristike sjemena procjenjivane su neposredno nakon uzimanja uzoraka te nakon otapanja duboko smrznutog sjemena (DSS), za sva 3 ekvilibracijska vremena. Utvrđeno je da prolongirani period ekvilibracije (24 sata i 48 sati) nije imao negativan učinak na kvalitetu DSS. Pokretljivost spermija, koja je ujedno i jedan od važnijih pokazatelja oplodnog potencijala bila je neznatno veća kod ekvilibracijskog perioda od 24h i 48h. Utvrđena je korelacija između vremena ekvilibracije i integriteta membrane te vijabilnosti spermija nakon dubokog smrzavanja pri čemu je 48-satni period ekvilibracije bio superiorniji u odnosu na onaj od 3 sata. U tom periodu (48 sati) ustanovljen je i veći postotak spermija s abnormalnostima glave u odnosu na period od 3 sata, ali ujedno i manji postotak abnormalnosti akrosome i spojnog dijela u odnosu na kontrolni period (3 sata). Rezultati ovog istraživanja pokazali su da je produženi period ekvilibracije jednako učinkovit kao i standardni trosatni period ekvilibracije te se može se jednako uspješno primjenjivati u standardnoj proizvodnji duboko smrznutog bičjeg sjemena, a u svrhu optimiziranja proizvodnje.

Abstract (english)

The aim of this study was to assess the effect of different equilibration times on cryopreservation of bull semen. For this purpose the ejaculates from 10 bulls which are used in regular production of frozen semen were used. The experiment was conducted on 60 ejaculates (6 per bull) in order to investigate the effect of different equilibration times on the quality of frozen-thawed semen. The ejaculate thus obtained included different equilibration periods: 3 hours (control sample) and 24 hours or 48 hours. Qualitative characteristics of the semen were evaluated immediately after its collection and after thawing for all of 3 equilibration periods. Prolonged equilibration time (24 and 48 hours) did not adversely affect the quality of frozen-thawed semen. Sperm motility, which is one of the most important indicators of fertilizing potential was slightly higher than those during the equilibration times of 24 or 48 hours. The correlation between equilibration period, membrane integrity and sperm viability after freezing was established, where 48 hours equilibration period was superior to that of 3 hours. In this period (48 hours), a larger percentage of sperm with head abnormalities was determined compared to the 3- hour period of equilibration, and also a smaller percentage of acrosome and midpiece abnormalities in comparison with the control period of equilibration (3 hours). The results of this research showed that prolonged period of equilibration is equally efficient as a standard 3- hour period and can be successfully applied in the standard production of frozen bull semen in order to optimize the production.

• Keywords: bik; ekvilibracija; duboko smrznuto sjeme; kvaliteta spermija
• Keywords (english): bull; equilibration; frozen thawed semen; sperm quality
• Language: croatian
• URN:NBN: urn:nbn:hr:178:628152
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-23 08:08:24