Izloženost pasa oblićima Dirofilaria sp.

master's thesis

• Title (english): Canine Exposure to Dirofilaria Sp Nematodes
• Author: Ana Aračić
• Mentor: Tatjana Živičnjak (mentor)
• Mentor: Franjo Martinković (mentor)
• Committee member: Albert Marinculić (predsjednik povjerenstva)
• Committee member: Krešimir Martinković (član povjerenstva)
• Committee member: Tatjana Živičnjak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Parasitology and Parasitic Diseases with Clinic); Zagreb
• Defense date and country: 2017-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Dirofilaria repens i Dirofilaria immitis su nitasti crvi roda Dirofilaria koji parazitiraju u mesojeda, a mogu se naći i u ljudi. Adulti D. immitis se u većini slučajeva nalaze u srcu i pulmonarnim arterijama, a D. repens u potkožju. Najveći broj invadiranih pasa su asimptomatski nositelji parazita tako da se dijagnoza dirofilarioze u većini slučajeva postavlja slučajno. Cilj ovog istraživanja je bilo utvrditi prisutnost i vrstu mikrofilarija Dirofilaria sp., prisutnost protutijela u istih pasa te dobivene nalaze međusobno usporediti kako bi se dobio uvid u stvarno stanje životinja koje su bile u kontaktu s oblićima Dirofilaria sp. Modificiranim Knott-ovim testom je dokazana prisutnost mikrofilarija, njihovim mjerenjem je utvrđena vrsta D. repens, a indirektnom imunoflourescencijom i prisutnost protutijela protiv mikrofilarija u pasa. Rezultati ovog istraživanja su pokazali višu seroprevalenciju od prevalencije mikrofilaremičnih pasa te višu seroprevalenciju od invadiranih životinja koje borave vani.

Abstract (english)

Dirofilaria repens and Dirofilaria immitis are nematode worms belonging to Dirofilaria genus which are mainly parasites of carnivores, but could be found in humans as well. D. immitis adults can be found mainly in heart and pulmonary arteries and D. repens in subcutaneous tissue. Most of the infected dogs are latent carrier of the parasite so final Dirofilaria infection is confirmed in most cases accidentally. The aim of this study was to determine microfilaria presence, microfilaria species and antibody presence which will show the real state of Dirofilaria sp. infested animals. The presence of microfilaria was proven with modified Knott’s test, their measurements revealed D. repens species and antibody prevalence with indirect immunofluorescence antibody test. The results of this survey showed higher seroprevalence than microfilaria prevalence in the same dog population. Higher prevalence was also in animals that spend most of their lives outside the house.

• Keywords: Dirofilaria immitis; Dirofilaria repens; Knott-ov test; indirektna imunoflourescencija
• Keywords (english): Dirofilaria immitis; Dirofilaria repens; Knott's test; indirect immunoflourescence.
• Language: croatian
• URN:NBN: urn:nbn:hr:178:113328
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-24 07:32:17