Proširenost i mogućnost dijagnostike infekcije uzrokovane vrstom Brucella ovis u ovaca u Republici Hrvatskoj

master's thesis

• Title (english): The Prevalence and the Possibility of Diagnosis of Infection Caused by Species Brucella ovis in Sheep in Croatia
• Author: Marija Perković
• Mentor: Zoran Milas (mentor)
• Mentor: Boris Habrun (mentor)
• Committee member: Nenad Turk (predsjednik povjerenstva)
• Committee member: Zoran Milas (član povjerenstva)
• Committee member: Boris Habrun (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Microbiology and Infectious Diseases with Clinic); Zagreb
• Defense date and country: 2016-09-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

U razdoblju od 2011. do 2015., godine istražena je rasprostranjenost infekcije vrstom
B. ovis u ovnova u Republici Hrvatskoj. U istraživanom razdoblju ukupno je pretraženo
18 324 seruma rasplodnih ovnova, a pozitivne reakcije su utvrđene u 1100 (6%) uzoraka.
Od 521 seruma pretraženih ovaca, pozitivne reakcije utvrđene su u 12 (2,3%). Kliničkim
pregledom adspekcijom i palpacijom testisa i epididimisa u 41,7% serološki pozitivnih
ovnova utvrđena je asimetrija skrotuma i jednostrano uvećanje repa epididimisa.
Patomorfološkim pregledom vidljive promjene poput granuloma, fibroze i atrofija testisa
i epididimisa utvrđene su u 58,3% pretražena testisa ovna. Bakteriološkom pretragom
iz uzoraka testisa i epididimisa ovnova izdvojeno je 20 (76,9%) izolata iz 26 pretraženih
ovnova. Svih 20 izolata molekularnim metodama identificirano je kao vrsta B. ovis. Na
temelju dobivenih rezultata vidljivo je da je zarazni epididimitis uzrokovan vrstom B. ovis
rasprostranjen u većini istraživanih regija u Republici Hrvatskoj.

Abstract (english)

In the period from 2011 to 2015, we examined the prevalence of infection by B. ovis
in rams in the Republic of Croatia. In the period of examination, a total of 18,324 serum
samples breeding rams, positive reactions were established in 1100 (6%) samples.
Of the 521 sera examined sheep, positive reactions were found in 12 (2.3%). Clinical
examination, palpation and adspection testes and epididymis in 41.7% seropositive
rams established the asymmetry of the scrotum and unilaterally increase the tail of
the epididymis. Pathological examination such as granulomas, fibrosis and atrophy of
the testes and epididymis were found in 58.3% searched ram testicles. Bacteriological
examination testicular and epididymal samples from rams, B. ovis isolated in 20 (76.9%)
from 26 searched rams. All 20 isolates by molecular methods have been identified as
species B. ovis. Based on the our results, it is evident that the infectious epididymitis
caused by B. ovis the most prevalent in the investigated region in the Republic of Croatia.

• Keywords: brucella; ovis; ovce; infekcija
• Keywords (english): brucella; ovis; sheeps; infection
• Language: croatian
• URN:NBN: urn:nbn:hr:178:295866
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-24 10:54:18