Oksidacijska stabilnost mlijeka krava i koza nakon intramamarne aplikacije propolisa

master's thesis

• Title (english): Oxidative Stability of Cow and Goat Milk after Intramammary Application of Propolis
• Author: Ana Hrdžić
• Mentor: Jasna Aladrović (mentor)
• Mentor: Lada Radin (mentor)
• Committee member: Antun Kostelić (predsjednik povjerenstva)
• Committee member: Lada Radin (član povjerenstva)
• Committee member: Jasna Aladrović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Physiology and Radiobiology); Zagreb
• Defense date and country: 2017-12-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Cilj ovog diplomskog rada bio je istraţiti utjecaj 1% i 5% bezalkoholne otopine
propolisa primijenjene intramamarno u lijeĉenju supkliniĉkih mastitisa na antioksidacijska
svojstva mlijeka krava i koza. Pripravak 1% nativnog propolisa apliciran je mlijeĉnim
kravama trokratno u sve ĉetvrti vimena i to poslije jutarnje muţnje, poslije veĉernje muţnje te
sljedeći dan poslije jutarnje muţnje. Koze ĉije je mlijeko bilo mikrobiološki pozitivno podijeli
smo u dvije skupine. Jednoj skupini koza trokratno smo aplicirali intramamarno otopinu 5%
nativnog propolisa, dok smo drugoj skupini trokratno aplicirali intramamarnu suspenziju
antibiotika klavuxila. U mlijeku su odreĊene aktivnosti glutation peroksidaze, superoksid
dismutaze, biološki antioksidacijski potencijal te koncentracija reaktivnih kisikovih
metabolita, kao pokazatelja oksidacijskog statusa. U uzorcima mlijeka koza aktivnost GSHPx,
SOD bila viša nego kod krava, a u uzorcima mlijeka koza koncentracija d-ROM nije bila
mjerljiva. Aplikacija propolisa dovela je do porasta aktivnosti GSH-Px kao i porasta omjera
GSH-Px/SOD u mlijeku koza nakon prve aplikacije te smanjenja koncentracije BAP u
mlijeku obje vrste ţivotinja. Aplikacija dvije doze propolisa kod krava dovela je do smanjenja
koncentracije d-ROM u mlijeku tretiranih ţivotinja dok se aktivnost SOD tijekom istraţivanja
nije mijenjala. Nakon trokratne aplikacije 1% propolisa u ĉetvrti krava koje su bile zahvaćene
supkliniĉkim mastitisom svi pretraţeni uzorci mlijeka bili su mikrobiološki negativni, a kod
koza 73% pretraţenih uzoraka mlijeka bilo je mikrobiološki negativno. Aplikacijom propolisa
dokazan je antioksidacijski i antimikrobni uĉinak propolisa apliciranog putem intramamarne
formulacije.

Abstract (english)

The aim of this study was to exemine effect of 1% and 5% non-alcoholic solution of
propolis applied intramammary during treatment of subclinical mastitis on antioxidant
properties of cow and goat milk. Solution of 1% native propolis was administered to milking
cows three times in all four quarters of udder, after morning milking, after evening milking
and next day after morning milking. Goats with positive microbiological findings was divided
in two groups. First group was treated with 5% solution of native propolis intramammary
three times, while the second group was treated with antibiotic klavuxil three times. Activities
of glutathione peroxidase and superoxide dismutase, biological antioxidant potential and
concentration of the reactive oxygen metabolites ware determined in milk. In samples of goat
milk activity of GSH-Px and SOD was higher than in cow milk, while concentration of d-
ROM in goat milk was not mesasurable. Application of propolis resulted in higher activity of
GSH-Px and also higher ratio of GSH-Px/SOD in goat milk after first application, while in
both goat and cow milk concentration of BAP was lower. Application of two doses of
propolis in cows resulted in lower d-ROM concentration in milk of treated animals while
activity od SOD during study show no changes. After applications of 1% propolis three times
in udder quarters that were affected with subclinical mastitis, all samples of cow milk were
microbiologically negative, while 73% of goat milk samples were microbiologically negative.
Application of propolis confirmed antioxidant and antimicrobial effect of intramammary
formulation of propolis.

• Keywords: mlijeko; krave; koze; supkliniĉki mastitis; propolis
• Keywords (english): milk; cows; goats; subclinical mastitis; propolis
• Language: croatian
• URN:NBN: urn:nbn:hr:178:905260
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-25 06:26:53