Utjecaj in vitro stimulacije perifernih mononuklearnih stanica krvi konkavalinom A na morfometrijske karakteristike limfocita

master's thesis

• Title (english): The Influence of In Vitro Stimulation of Peripheral Mononuclear Blood Cells with Concavaline A on Morphometric Characteristics of Lymphocytes
• Author: Mira Šokičić
• Mentor: Nina Poljičak-Milas (mentor)
• Mentor: Kristina Matković (mentor)
• Committee member: Mirna Robić (predsjednik povjerenstva)
• Committee member: Kristina Matković (član povjerenstva)
• Committee member: Nina Poljičak-Milas (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Pathophysiology); Zagreb
• Defense date and country: 2017-06-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Opća proliferativna sposobnost T-limfocita procjenjuje se invitro stimulacijom biljnim mitogenima, fitohemaglutininom (PHA) i konkavalinom A (ConA). Promjene nastale izlaganjem mitogenima i stimulacijom imunosnog sustava, mogu dovesti do povećanja i/ili abnormalnosti stanice i jezgre, te promjene njihovog meĊusobnog odnosa, što se moţe kvantificirati morfometrijom. Na ukupno 18 krvnih razmaza mjerene su morfometrijske karakteristike limfocita prije i nakon aktivacije konkavalinom A. Digitalna analiza krvnih stanica napravljena je na osobnom raĉunalu s podrţavajućim programom SFORM (VAMSTEC, Zagreb). Rezultati su pokazali znaĉajno povećanje površine, opsega, maksimalnog polumjera, konveksne površine te faktora pravilnosti limfocita nakon inkubacije s mitogenom u usporedbi s istim pokazateljima stanice limfocita prije inkubacije. Vrijednosti minimalnog polumjera i duţine stanice bile su podjednake u limfocitima prije i nakon inkubacije. Kod mjerenja morfometrijskih pokazatelja jezgara, nisu izmjerene znaĉajne promjene njihove površine ili opsega, meĊutim utvrĊen je znaĉajno smanjem minimalni polumjer i širina jezgre, znaĉajno manji N/C omjer te smanjena vrijednost faktora oblika (FF). Dobiveni rezultati pokazali su da promjene na stanicama limfocita i njihovim jezgrama upućuju na metaboliĉku aktivnost pri tretiranju mitogenima te morfometrijska analiza moţe pruţiti vrijedne podatke u procjeni transformacije imunokompetentnih stanica.

Abstract (english)

General proliferative ability of T-cells estimates with in vitro stimulation with plant mitogens, phytohemaglutinin (PHA) and concavalin A (ConA). Changes caused by exposure to mitogens and stimulation of the immune system may lead to an increase and/or abnormality of the cell and nucleus, and a change in their mutual relationship, which can be quantified by morphometry. At the total of 18 blood smears the morphometric characteristics of the lymphocytes before and after activation with concavalin A were measured. The digital blood cell analysis was performed on a personal computer using the support program SFORM (VAMSTEC, Zagreb). The results showed a significant increase in surface, bandwidth, maximum radius, convex surface, and lymphocyte form factor after incubation with mitogen compared to the same lymphocyte cell pre-incubation ratios. The minimum radius and cell length values were consistent in lymphocytes before and after incubation. When measuring the morphometric parameters of the nuclei, no significant changes in their surface or extent were measured, however, there was significant reduction in the minimum radius and core width, significantly lower N / C ratio and reduced factor factor (FF). The obtained results showed that changes in lymphocyte cells and their nuclei indicate metabolic activity in the treatment of mitogens, and morphometric analysis can provide valuable data in estimating the transformation of immunocompetent cells.

• Keywords: limfociti; morfometrija; konkavalin A; in vitro inkubacija
• Keywords (english): lymphocytes; morphometry; concavalin A; in vitro incubation
• Language: croatian
• URN:NBN: urn:nbn:hr:178:170016
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-25 06:30:57