Metabolički i oksidacijski stres konja prilikom utrka daljinskog jahanja

master's thesis

• Title (english): Metabolic and Oxidative Stress in Endurance Horses during Racing
• Author: Karla Klobučar
• Mentor: Nika Brkljača Bottegaro (mentor)
• Mentor: Zoran Vrbanac (mentor)
• Committee member: Jelena Gotić (predsjednik povjerenstva)
• Committee member: Nika Brkljača Bottegaro (član povjerenstva)
• Committee member: Zoran Vrbanac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Surgery, Orthopaedics and Ophtalmology Clinic); Zagreb
• Defense date and country: 2018-09-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Daljinsko jahanje fizički je najzahtjevnija disciplina konjičkog sporta. U istraživanje
su uključena 53 konja koja su uspješno završila utrku na jednome od četiri promatrana
natjecanja daljinskog jahanja u Republici Hrvatskoj. Uspoređivana je prosječna brzina
kretanja i prosječno vrijeme oporavka konja u odnosu na dob, spol, pasminu i iskustvo te
dužinu staze. Konjima su u svrhu istraživanja metaboličkog i oksidacijskog stresa uzimana
dva uzorka krvi, prije i nakon utrke. Istraživani su odabrani serumski biokemijski parametri, a
kao pokazatelji oksidacijskog stresa korišteni su test determinacije reaktivnih metabolita
kisika (d-ROMs), malondialdehid (MDA) i biološki antioksidansni potencijal (BAP). Konji
arapske pasmine imali su kraće vrijeme oporavka. Dob, spol i iskustvo konja nisu imali
znatan utjecaj na vrijednosti promatranih parametara. Vrijednosti kreatin kinaze i glukoze u
krvi (GUK) prije utrke bile su više od referentnih vrijednosti. Vrijednosti bubrežnih
parametara i parametara mišićnog oštećenja bilježe porast poslije utrke te je razlika znatnija
na većim dužinama utrka. Povećanjem dužine utrke povećava se i gubitak elektrolita. Znatan
porast vrijednosti svih promatranih pokazatelja oksidacijskog stresa zabilježen je poslije
utrke. BAP bilježi veći porast na utrkama od 80 km u odnosu na kraće utrke, a s njegovim
porastom proporcionalno su rasle vrijednosti ureje i triglicerida. MDA i GUK proporcionalno
su rasli, što upućuje da zabilježena hiperglikemija utječe na lipidnu peroksidaciju stanice.
Utrke daljinskog jahanja uzrokuju pojačane zahtjeve organizma koji se očituju
povećanim gubitkom elektrolita, aktivnosti mišićnog i mokraćnog sustava te oksidacijskim
stresom.

Abstract (english)

Endurance riding is the most physically demanding discipline of equine sport. The
study included 53 horses that successfully completed the race in one of the four observed
endurance riding competitions in Croatia. The average speed and recovery time were
compared to age, sex, breed, experience and length of the race track. Two blood samples were
taken from horses for the purpose of metabolic and oxidative stress research, one before and
one after the race. The analysis consisted of several serum biochemical parameters, as well as
determination of reactive oxygen metabolites (d-ROMs), malondialdehyde (MDA) and
biological antioxidant potential (BAP) as indicators of oxidative stress. Horses of Arabian
breeds had shorter recovery time. Age, sex and horse experience did not have a significant
influence on the studied parameters. Creatine kinase and blood glucose values prior to the
race were higher than reference values. Values of renal parameters and parameters of muscle
damage increased after races, and the degree of change was more significant at longer racing
lengths. Electrolyte loss was more pronounced during longer races. Significant increase in all
observed oxidative stress markers were recorded after the race. BAP has recorded higher
values during longer races. Positive correlations were recorded for BAP, urea and triglyceride
concentrations. MDA and blood glucose have increased proportionally, indicating that
hyperglycemia affected cells lipid peroxidation.
Endurance riding causes increased body requirements that are manifested by increased
electrolyte loss, muscular and urinary system activity, and oxidative stress.

• Keywords: daljinsko jahanje; konj; metabolizam; oksidacijski stres
• Keywords (english): endurance riding; equine; metabolism; oxidative stress
• Language: croatian
• URN:NBN: urn:nbn:hr:178:051584
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-01-08 07:33:22