Utvrđivanje prisutnosti bakterije Anaplasma Phagocytophilum u populaciji mišolikiih glodavaca

master's thesis

• Title (english): Prevalence of Anaplasma Phagocytophilum in Wild Rodent Population
• Author: Ines Papak
• Mentor: Suzana Hađina (mentor)
• Committee member: Zrinka Štritof (predsjednik povjerenstva)
• Committee member: Josipa Habuš (član povjerenstva)
• Committee member: Suzana Hađina (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Microbiology and Infectious Diseases with Clinic); Zagreb
• Defense date and country: 2019-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Anaplazmoza je zarazna bolest koju uzrokuju bakterije iz roda Anaplasma. A. phagocytophilum uzročnik je anaplazmoze konja, krpeljne groznice preživača, granulocitne anaplazmoze pasa i mačaka te humane granulocitne anaplazmoze u ljudi. Srna i divlji glodavci prirodni su rezervoari A. phagocytophilum, a osim toga glodavci su i domaćini svih razvojnih stadija krpelja. Cilj ovog istraživanja bio je utvrditi prisutnost bakterije A. phagocytophilum u populaciji mišolikih glodavaca. Uzorci tkiva slezene pretraženi su ugniježđenom lančanom reakcijom polimeraze, te su pozitivni rezultati utvrđeni u 15% uzoraka. U svrhu provjere utvrđivanja vrste A. phagocytophilum parovima početnica EC9/EC12a i SSAP2f/SSAP2r korištenih u ovom istraživanju, nasumično je odabrano pet pozitivnih uzoraka te poslano na sekvenciranje. Sekvence su analizirane BLAST programom kojim je utvrđena 100% podudarnost s bakterijskom vrstom iz porodice Anaplasmataceae, Candidatus Neoehrlichia mikurensis. Rezultati ovog preliminarnog istraživanja pokazali su da se bez sekvenciranja svih pozitivnih uzoraka te korištenja više različitih parova početnica ne može utvrditi stvarna prisutnost bakterije A. phagocytophilum. Kod određenih životinjskih vrsta na određenom području javljaju se unakrižne reakcije s drugim bakterijskim vrstama iz porodice Anaplasmataceae.

Abstract (english)

Anaplasmosis is an infectious disease caused by bacteria from the genus Anaplasma. A. phagocytophilum is the cause of equine anaplasmosis, ruminant tick fever, canine and feline granulocytic anaplasmosis, and human granulocytic anaplasmosis. Deer and wild rodents are natural reservoirs of A. phagocytophilum, and in addition, rodents are also host tick development stages. The aim of this study was to determine the presence of A. phagocytophilum in a wild rodent population. Spleen tissue samples were examined by nested polymerase chain reaction and positive results were found in 15% of the samples. For the purpose of confirming the determination of A. phagocytophilum by EC9/EC12a and SSAP2f/SSAP2r primers used in this study, five positive samples were randomly selected and sent for sequencing. The sequences were analyzed by a BLAST program that determined 100% identity with Candidatus Neoehrlichia mikurensis, a bacterial species belonging to the family Anaplasmataceae,. The results of this preliminary study showed that without sequencing all positive samples and using different primer sets the presence of A. phagocytophilum could not be determined. In certain animal species in certain geographic area there is a possibility of cross-reactions occurring with other bacterial species in the family Anaplasmataceae.

• Keywords: Anaplasma Phagocytophilum; mišolikii glodavci
• Keywords (english): Anaplasma Phagocytophilum; wild rodent
• Language: croatian
• URN:NBN: urn:nbn:hr:178:545203
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-01-28 12:49:06