| Abstract | Potrošnja mlijeka iz mljekomata popularna je u Hrvatskoj no potrebno je poznavati
potencijalne rizike za potrošače u pogledu mikrobiološke sigurnosti. U radu je cilj bio ispitati
prisutnost i brojnost koagulaza pozitivnih stafilokoka u mlijeku iz mljekomata (n=319), te
testirati osjetljivost izolata na antimikrobne tvari tijekom jednogodišnjeg razdoblja. Broj
koagulaza pozitivnih stafilokoka određen je prema HRN EN ISO 6888, a procjena otpornosti
na kanamicin, eritromicin, nitrofurantoin, ampicilin, levofloksacin, teicoplanin, ciprofloksacin,
penicilin, cefotaksim, tetraciklin, trimetoprim/sulfametoksazol, linezolid, kloramfenikol i
ceftazidim pomoću disk difuzijskog testa. Prosječni broj koagulaza pozitivnih stafilokoka
prema mjesecima uzorkovanja se kretao između 2,04 do 3,09 log cfu/ml. Ukupno je 88 uzoraka
sadržavalo ove bakterije, što čini 27,58 %. Osjetljivost na antimikrobne tvari testirana je kod
60 izolata, a postotak rezistentnih se ovisno o mjesecu uzorkovanja kretao od 33,3 do 100 %.
Ukupno je 41 izolat bio otporan na najmanje jednu antimikrobnu tvar (68,33 %). Najveći broj
izolata koagulaza pozitivnih stafilokoka bio je otporan na penicilin i ampicilin te
ciprofloksacin, a potom linezolid, ceftazidim, kanamicin i levofloksacin. |
| Abstract (english) | Consumption of milk from vending machines is popular in Croatia, but it is necessary
to evaluate the potential risks to consumers in terms of microbiological safety. The aim of this
study was to examine the presence and counts of coagulase-positive staphylococci in raw milk
from vending machines (n = 319), and to test the susceptibility of isolates to antimicrobials over
a one-year period. The number of coagulase-positive staphylococci was determined according
to HRN EN ISO 6888, and the assessment of resistance to kanamycin, erythromycin,
nitrofurantoin, ampicillin, levofloxacin, teicoplanin, ciprofloxacin, penicillin, cefotaxime,
tetracycline, trimethoprim / sulfamethoxidolol, linezolid, chloramphenicol and ceftazidim by
disk diffusion method. The average number of coagulase-positive staphylococci by months of
sampling ranged from 2.04 to 3.09 log cfu / ml. A total of 88 samples contained these bacteria
(27.58 %). Sensitivity to antimicrobials was tested in 60 isolates and the percentage of resistant
isolates varied from 33.3 to 100 % depending on the sampling month. A total of 41 isolates
were resistant to at least one antimicrobial agent (68.33 %). The majority of coagulase-positive
staphylococci were resistant to penicillin, ampicillin and ciprofloxacin, followed by linezolid,
ceftazidime, kanamycin and levofloxacin |
