Proširenost bakterija iz kompleksa Borrelia Burgdorferi sensu lato unutar populacije mišolikih glodavaca

master's thesis

• Title (english): Prevalence of Borrelia Burgdorferi Sensu Lato Complex in Small Rodents
• Author: Tea Ćorić
• Mentor: Josipa Habuš (mentor)
• Committee member: Suzana Hađina (predsjednik povjerenstva)
• Committee member: Zrinka Štritof (član povjerenstva)
• Committee member: Josipa Habuš (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Microbiology and Infectious Diseases with Clinic); Zagreb
• Defense date and country: 2020-05-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Lymska borelioza je bolest endemskih žarišta koja se javlja u ljudi i životinja, a uzrokuju je različite vrste patogenih bakterija unutar kompleksa B. burgdorferi s.l. Epizootiološki ciklus Lymske borelioze u prirodi uključuje horizontalni prijenos između vektora i rezervoara. U Europi, glavni vektor je krpelj Ixodes ricinus, dok su mišoliki glodavci poznati kao glavni rezervoari ove bakterije. Ciljevi ovog istraživanja bili su utvrditi učestalost infekcije u mišolikih glodavaca u nekoliko endemskih područja Lymske borelioze u RH te utvrditi eventualnu povezanost pojedinih vrsta glodavaca sa određenim vrstama bakterija unutar kompleksa Borrelia burgdorferi s.l. Lančanom reakcijom polimerazom pretraženo je 197 uzoraka mokraćnih mjehura mišolikih glodavaca. Korištenjem „touchdown“ PCR metode uz parove početnica koje umnažaju veliki odsječak flaB gena nismo uspjeli dokazati prisustvo bakterije B. burgdorferi u niti jednom uzorku. Nasumično smo odabrali 20/197 (10 %) uzoraka mokraćnih mjehura te ih ponovno pretražili „touchdown“ PCR metodom korištenjem početnica koje umnažaju mali odsječak flaB gena te ugniježđenom PCR metodom korištenjem početnica koje umnažaju odsječak ospA gena. Korištenjem oba protokola u dva (10 %) uzoraka mokraćnih mjehura, dokazano je prisustvo bakterije B. burgdorferi. Dodatno, pretraženi su i drugi organi pozitivnih glodavaca, a DNK borelije dokazali smo još samo u uškama. S obzirom na broj zabilježenih slučajeva oboljenja ljudi u Hrvatskoj i činjenice da je Lymska borelioza druga najčešća zoonoza u Hrvatskoj, potrebna su daljnja istraživanja na rezervoarima i vektorima bakterije B. burgdorferi kako bi se utvrdila stvarna prevalencija bakterija iz kompleksa B. burgdorferi s.l. u tih životinja koje su bitne za održavanje enzootskog ciklusa Lymske borelioze unutar RH.

Abstract (english)

Lyme borreliosis is an endemic foci disease which occurs in humans and animals and it is caused by various species of pathogenic bacteria within B. burgdorferi s.l. complex. The epizootiological cycle of Lyme borreliosis in nature involves horizontal transmission between vectors and reservoirs. In Europe, the major vector is tick Ixodes ricinus, while small rodents are known as the major reservoirs of B. burgdorferi. The aim of this study was to determine the frequency of infection in small rodents in several endemic areas of Lyme borreliosis in the Republic of Croatia and to determine the possible association of certain rodent species with the particular bacterial species within the Borrelia burgdorferi s.l. complex. Polymerase chain reaction was used to test 197 urinary bladder samples of small rodents. All the samples tested negative for the presence of B. burgdorferi when using touchdown PCR method and primer pairs targeting the large part of flaB gene. We randomly selected 20/197 (10%) urinary bladder samples and tested them again by using touchdown PCR method and primer pairs targeting the small part of flaB gene and also by using nested PCR method and primer pairs targeting the ospA gene. Two (10%) samples of urinary bladder tested positive for the presence of B. burgdorferi when using both protocols. Additionally, other organ samples from both positive rodents were tested and only the samples of ear tissues tested positive for borrelial DNA. Given the number of reported human cases in Croatia and the fact that Lyme borreliosis is the second most common zoonosis in Croatia, further studies on B. burgdorferi reservoirs and vectors are needed to determine the real prevalence of bacterial species within B. burgdorferi s.l. complex in these animals that are essential for maintaining the enzootic cycle of Lyme borreliosis in the Republic of Croatia.

• Keywords: Lymska borelioza; mišoliki glodavci; PCR
• Keywords (english): Lyme borreliosis; small rodents; PCR
• Language: croatian
• URN:NBN: urn:nbn:hr:178:639236
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-12-09 16:34:33