Title Oksidacijska stabilnost različitih tkiva sivog puha (Glis glis) Title (english) Oxidative Stability of Liver, Kidneys, Heart and Muscle of the Gray Dormouse (Glis glis) Author Loredana Pincan Mentor Jasna Aladrović (mentor)Mentor Dean Konjević (mentor)Committee member Lana Pađen (predsjednik povjerenstva)Committee member Jasna Aladrović (član povjerenstva)Committee member Dean Konjević (član povjerenstva)Granter University of Zagreb Defense date and country 2020-10-06, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Abstract Sivi puh (Glis glis L.) je autohtona sitna divljač iz reda glodavaca, porodice puhova. Metabolizam puhova je tijekom proljeća i ljeta vrlo intenzivan, pare se, donose na svijet mlade te se pojačano hrane kako bi se pripremili za hibernaciju. Pri fiziološkim uvjetima proizvodnja reaktivnih kisikovih spojeva i drugih molekula koje izazivaju oksidacije u organizmu te koncentracija/aktivnost antioksidativnim molekulama su u ravnoteži i ovise o intenzitetu metaboličkih procesa. Stoga je cilj ovog istraživanja bio utvrditi aktivnost enzima glutation perokisidaze (GSH-Px), paraoksonaze 1 (PON1) i superoksid dismutaze (SOD), kocentraciju neenzimskih antioksidacijskih molekula pomoću biološkog antioksidacijskog potencijala (BAP) te koncentraciju reaktivnih kisikovih metabolita (d-ROM) u jetrinom, bubrežnom, srčanom i mišićnom tkivu ženki i mužjaka sivog puha neposredno pred hibernaciju. Istraživanje je provedeno na tkivima 33 jedinke sivoga puha, 18 ženki i 15 mužjaka. Uzorci su prikupljeni tijekom sezone legalnog odstrela (rujan - listopad 2017.). Najveće aktivnosti GSH-Px i PON1 utvrđene su u jetri dok je najmanja aktivnost zabilježena u tkivu m. gluteus sivog puha. U istraživanim tkivima nije utvrđena značajna razlika među spolovima (p>0,05). Najveća aktivnost SOD utvrđena je u tkivu bubrega, a najmanja aktivnost zabilježena je u tkivu srca sivog puha. Aktivnost SOD nije se značajno razlikovala u istraženim tkivima kod ženki i mužjaka sivog puha (p>0,05). Zabilježena je najveća koncentracija BAP u tkivu m. gluteus dok je najmanja koncentracija utvrđena u srčanom tkivu sivog puha. U istraživanim tkivima nije utvrđena statistički značajna razlika u vrijednostima kod ženki i mužjaka (p>0,05). Najveća koncentracija d-ROM utvrđena je u tkivu jetara, a najmanja u tkivu srca sivog puha. Koncentracija d-ROM u m. gluteus i srcu bila je značajno veća u mužjaka u odnosu na ženke (p<0,05) dok su koncentracije u jetri i bubrezima bile približno jednake. Na osnovi rezultata možemo zaključiti da su oksidativni i antioksidativni procesi najintenzivniji u jetri i bubrezima što odgovara intenzitetu metaboličkih procesa ovih tkiva. Najveća koncentracija BAP u m. gluteus uz istovremeno najmanju aktivnost antiokisdativnih enzima ukazuje na kompenzatorni učinak neezimskih antioksidacijskih molekula. Intenzivniji oksidacijski procesi u srcu mužjaka potvrđuju da mužjaci imaju višu frekvenciju srčanog rada a u m. gluteus ukazuju na veću mišićnu aktivnost u odnosu na ženke sivog puha. Izostanak većeg broja značajnih razlika može se objasniti time da su jedinke u ovom istraživanju odstrjeljene u rujnu-listopadu, dakle u vrijeme kada su najveći primjerci ove vrste već u pušinama, radi se o mlađim, lakšim jedinkama koje vjerojatno nisu spolno zrele.
Abstract (english) Gray dormouse (Glis glis L.) is an autochthonous small game from the order of rodents, family of dormice. Metabolism of the dormouse is very intense during spring and summer, they mate, give birth to their young and feed intensively to prepare for hibernation. Under physiological conditions, production of reactive oxygen species and other molecules that cause oxidation in the body and the concentration / activity of antioxidant molecules are in equilibrium and depend on the intensity of metabolic processes. Therefore, the aim of this study was to determine activity of the enzymes glutathione peroxidase (GSH-Px), paraoxonase 1 (PON1) and superoxide dismutase (SOD), concentration of non-enzymatic antioxidant molecules using biological antioxidant potential (BAP) and the concentration of reactive oxygen metabolites (d-ROM) in liver, kidney, heart, and muscle tissue of female and male gray dormice just before hibernation. The study was conducted on the tissues of 33 individuals of gray dormice, of which 18 were females and 15 males. Samples were collected during the legal hunting season (September - October 2017). The highest activities of GSH-Px and PON1 were found in the liver tissue while the lowest activity was recorded in the gluteus tissue of the gray dormouse. No significant difference was found among genders in the studied tissues (p> 0.05). The highest SOD activity was found in kidney tissue, and the lowest activity was recorded in gray dormouse heart tissue. SOD activity did not differ significantly in the studied tissues in females and males of gray dormouse (p> 0.05). The highest concentration of BAP was recorded in gluteus tissue, while the lowest concentration was found in gray dormouse heart tissue. No statistically significant difference was found in values in females and males in the researched tissues (p> 0.05). The highest concentration of d-ROM was found in liver tissue, and the lowest in gray dormouse heart tissue. Concentration of d-ROM in gluteus and heart was significantly higher in males compared to females (p <0.05) while the concentrations in liver and kidneys were approximately equal. Based on the results, it can be concluded that oxidative and antioxidant processes are most intense in liver and kidneys, which corresponds to the intensity of metabolic processes in these tissues. The highest concentration of BAP in the gluteus muscle with the lowest activity of antioxidant enzymes indicates a compensatory effect of non-enzymatic antioxidant molecules. More intense oxidative processes in the heart of males confirm that males have a higher heart rate and in the m. gluteus indicate greater muscle activity compared to females of gray dormouse. Absence of a higher number of significant differences can be explained by the fact that the individuals in this study were hunted in September-October, ie at a time when the largest specimens of this species are already in the den, they are younger, lighter in mass individuals that are probably not yet reproductively mature.
Keywords
sivi puh
tkiva
antioksidacijske molekule
reaktivni kisikovi metaboliti
Keywords (english)
gray dormouse
tissues
antioxidant molecules
reactive oxygen metabolites
Language croatian URN:NBN urn:nbn:hr:178:498305 Study programme Title: Veterinary Medicine Type of resource Text File origin Born digital Access conditions Open access Terms of use Created on 2020-12-09 18:05:15
