Učestalost izlučivanja bakterija roda Salmonella u konja s kliničkim znakovima kolike i konja liječenih na Veterinarskom fakultetu

master's thesis

• Title (english): Prevalence of Salmonella Spp. Shedding in Horses with Clinical Signs of Colic and Hospitalized Horses
• Author: Melita Majhut
• Mentor: Zrinka Štritof Majetić (mentor)
• Mentor: Nika Brkljača Bottegaro (mentor)
• Committee member: Zoran Milas
• Committee member: Nika Brkljača Bottegaro
• Committee member: Zrinka Štritof Majetić
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Microbiology and Infectious Diseases with Clinic); Zagreb
• Defense date and country: 2015-12-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Infekcije salmonelama u konja mogu biti subkliniĉke, a mogu se i oĉitovati raznim kliniĉkim znakovima, ponajprije vrućicom, kolikom, leukopenijom i proljevom. Glavni problem u prepoznavanju i sprjeĉavanju širenja salmoneloze u konja su subkliniĉki inficirani konji, koji povremeno izluĉuju bakterije izmetom. U subkliniĉki inficiranih konja razvoj kliniĉke salmoneloze ovisi o raznim predisponirajućim ĉimbenicima, kao što su transport, prenatrpanost, nagle promjene u prehrani, operacije, gastrointestinalni poremećaji i lijeĉenje antibioticima. Do sada u Republici Hrvatskoj nisu provoĊena istraţivanja infekcija vrsno nespecifiĉnim salmonelama u konja te nema nikakvih podataka o uĉestalosti subkliniĉkih infekcija kao niti o pojavnosti bolesti. Cilj ovog istraţivanja bio je odrediti uĉestalost izluĉivanja bakterija roda Salmonella u konja lijeĉenih na klinikama Veterinarskog fakulteta i konja s kliniĉkim znakovima kolike lijeĉenih na terenu. Prikupljeno je 162 uzoraka izmeta od 62 konja. Uzorci su uzeti od svakog konja u razmaku od 24 sata. Kultivirani su korištenjem tehnika obogaćivanja i selektivnog medija za izolaciju bakterija roda Salmonella iz izmeta. Sumnjive kolonije su nadalje identificirane uobiĉajenim biokemijskim metodama. Salmonella sp. izdvojena je iz 1/62 (1,61%) pretraţenih konja. Relativno niska uĉestalost izluĉivanja utvrĊena ovim istraţivanjem moţebitno je posljedica ĉinjenice da je tek u manjeg broja pretraţenih konja bilo moguće uzorkovati preporuĉenih pet uzoraka izmeta. Naime, budući da se salmonela izmetom izluĉuje intermitentno, najmanje pet uzoraka je potrebno da se postigne zadovoljavajuća osjetljivost pretrage. U ovom istraţivanju prvi je puta izdvojena Salmonella sp. iz izmeta konja u Republici Hrvatskoj, a kako je izdvojena iz konja s tipiĉnim kliniĉke znakovima salmoneloze (vrućica, kolika, leukopenija), ujedno je i prvi puta postavljena objektivna dijagnoza bolesti. Dobiveni rezultati trebali bi posluţiti podizanju svijesti veterinara o prisutnosti ove bolesti te postaviti temelj za provoĊenje biosigurnosnih mjera u veterinarskim ustanovama pri zaprimanju konja u kojih su prisutni riziĉni ĉimbenici za izluĉivanje salmonela, a posebice konja u kojih postoji kliniĉka sumnja na salmonelozu.

Abstract (english)

Salmonella infection in horses can be subclinical and they can also manifest various clinical signs, especially fever, colic, diarrhea and leukopenia. The main problem in recognizing and preventing the spread of salmonellosis in horses are subclinical infected horses which occasionally shed bacteria in feces. Developing clinical salmonellosis in subclinical infected horses depends on a variety of predisposing factors such as transportation, overcrowding, sudden changes in diet, surgery, gastrointestinal disorders and antibiotic treatment. There were no researches conducted in non-specific infections salmonella in horses so far in Croatia and there are no data on the frequency of subclinical infection nor on diseases. The aim of this study was to determine the prevalence of Salmonella shedding in horses treated in clinics in the Veterinary Faculty and horses with clinical signs of colic treated in the field. There were 162 faecal samples collected from 62 horses. Samples were taken from each horse in the frequency of 24 hours. They were cultured using enrichment techniques and selective media for the isolation of Salmonella from feces. Suspicious colonies were further identified by conventional biochemical methods. Salmonella sp. was isolated from 1/62 (1.61%) examined horses. The relatively low prevalence of shedding determined in this study is possibly due to the fact that it was possible to sample the recommended five samples of feces only from a small number of examined horses. A minimum of five samples is required to achieve a satisfactory sensitivity of the examination, because Salmonella sheds intermittently. This research includes the first isolated Salmonella sp. from the faeces of horses in Croatia, and as it is isolated from the horse with typical clinical signs of salmonellosis (fever, colic, leukopenia), is also the first time that an objective diagnosis of the disease was determined. The results should serve to raise awareness about the presence of the disease to veterinarians and to lay the foundation for the implementation of biosecurity measures in the veterinary institutions with receiving horses in which they are present risk factors for shedding of Salmonella, especially horses in which there is a clinical suspicion of salmonellosis.

• Keywords: salmoneloza; konji; kolike
• Keywords (english): salmonellosis; horses; colic
• Language: croatian
• URN:NBN: urn:nbn:hr:178:614933
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-01-12 12:58:01