UTJECAJ PODTIPA PSEĆEG PARVOVIRUSA 2 NA KLINIČKU SLIKU, TIJEK I ISHOD LIJEČENJA

master's thesis

• Title (english): INFLUENCE OF DOG PARVOVIRUS SUBTYPE 2 ON THE CLINICAL PICTURE, COURSE AND OUTCOME OF TREATMENT
• Author: Dorotea Patačko
• Mentor: Vladimir Stevanović (mentor)
• Committee member: Ljubo Barbić (predsjednik povjerenstva)
• Committee member: Vilim Starešina (član povjerenstva)
• Committee member: Vladimir Stevanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2021-03, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Public Health and Food Safety

Abstract

Parvovirusna infekcija pasa prvi puta je opisana krajem prošlog stoljeća, a danas je jedna od najvažnijih zaraznih bolesti u ove vrste. Izrazita sklonost mutacijama dovodi do pojave novih sojeva koji istiskuju starije u populaciji pasa.
Za ovo istraživanje sakupljeno je i obrađeno ukupno 78 uzoraka fecesa i obrisaka rektuma pasa zaprimljenih na Kliniku za zarazne bolesti Zavoda za mikrobiologiju i zarazne bolesti s klinikom Veterinarskog fakulteta Sveučilišta u Zagrebu. Navedeno uzorkovanje i prikupljanje
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podataka o životinjama uključivalo je razdoblje od 1. siječnja 2019. do 1. prosinca 2020. Sve životinje, odnosno psi pokazivali su znakove akutnog gastroenteritisa. Dostupni podaci o nacionalu, anamnezi, kliničkom pregledu te tijeku i ishodu liječenja korišteni su u svrhu statističke obrade. Metodom lančane reakcije polimeraze u uzorcima fecesa ili obriscima rektuma dokazivana je prisutnost psećeg parvovirusa-2.
Nakon određivanja nukleotidnog slijeda umnoženih odsječaka virusnog genoma ustanovljena je prisutnost sva tri poznata podtipa CPV-2 virusa, a najzastupljeniji su podtipovi CPV-2c (53,85%) i CPV-2a (35,9%). Iz dodatnih anamnestičkih podataka bilo je vidljivo da se podtip CPV-2c češće javlja kod pasa iz azila. Isti podtip se javlja češće u štenadi, dok se preostala dva javljaju češće u starijih životinja. Težina kliničke slike, tijek i ishod liječenja ne ovise o podtipu virusa. Cijepljenje daje zaštitu pasa neovisno o podtipu virusa. Psi koji razviju kliničku sliku, koja upućuje na parvovirozu, zahtjevaju agresivan pristup liječenju neovisno o cjepnom statusu.

Abstract (english)

Parvovirus infection in dogs was first described at the end of the previous century. Due to the high rate of mutations, new subtypes often emerge and become dominant in the dog population. For this research, a total of 78 faeces and rectal swabs of dogs were collected and processed at the Clinic for Infectious Diseases of the Department of Microbiology and Infectious Diseases with the Clinic of the Faculty of Veterinary Medicine, University of Zagreb. This sampling and collection of data on animals included the period from 1 January 2019 to 1 December 2020. All animals and dogs showed signs of acute gastroenteritis. Data
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on nationality, medical history, clinical examination, and course and outcome of treatment were used for statistical analysis. The presence of canine parvovirus-2 was demonstrated by a polymerase chain reaction in faecal samples or rectal swabs.
After determining the nucleotide sequence of the amplified segments of the viral genome, the presence of all three known subtypes of the CPV-2 virus was established, and the most common subtypes are CPV-2c (53.85%) and CPV-2a (35.9%). Additional anamnestic data showed that the CPV-2c subtype was more common in shelter dogs. The same subtype is more commonly responsible for infections in puppies, while the other two are common in older dogs.
Assessment of the clinical picture, course and outcome of disease showed that there was no significant difference between individual virus subtypes. Immunoprophylaxis gives the same level of protection for all three subtypes. Finally, parvovirosis demands aggressive therapy regardless of the vaccinal status.

• Keywords: parvovirusna infekcija pasa; anamnestički podaci; podtip CPV-2; klinički slika; imunoprofilaksa
• Keywords (english): canine parvovirus infection; anamnestic data; CPV-2 subtype; clinical picture; immunoprophylaxis
• Language: croatian
• URN:NBN: urn:nbn:hr:178:866973
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-04-27 11:52:08