Učinak ekstrakata propolisa na markere cijeljenja rana u staničnoj kulturi keratinocita

master's thesis

• Title (english): EFFECT OF PROPOLIS EXTRACTS ON WOUND HEALING MARKERS IN HUMAN IMMORTALIZED KERATINOCYTE CELL CULTURE
• Author: Sunčica Sertić
• Mentor: Jelena Šuran (mentor)
• Mentor: Josipa Vlainić (mentor)
• Committee member: Frane Božić (predsjednik povjerenstva)
• Committee member: Andreja Prevendar Crnić (član povjerenstva)
• Committee member: Jelena Šuran (član povjerenstva)
• Committee member: Ivana Tlak Gajger (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2021-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Public Health and Food Safety

Abstract

Propolis djeluje imunomodulatorno, antimikrobno, antioksidativno, analgetsko i protuupalno čime potiče brže cijeljenje rana. Važni spojevi u propolisu su flavonoidi, fenilpropenoidi te fenolične kiseline poput kumarične kiseline, kafeične kiseline te ferulične kiseline. Fenetil ester kofeinske kiseline (CAPE) imunosupresivno djeluje inhibirajući rast bakterija, sprječavajući staničnu podjelu. Antioksidativno djelovanje propolisa potiče visoka koncentracija polifenola i flavonoida. Bezalkoholni ekstrakt propolisa pokazuje antimikrobni utjecaj na gram pozitivne bakterije i gram negativne bakterije. Propolis pokazuje antimikotični učinak prema gljivicama. MTT testom citotoksičnosti dokazana je neškodljivost propolisa prema stanicama. S obzirom na rezultate scratch testa 1,5%, 1% i 0,5% koncentrirane otopine propolisa postigle su statistički značajno brže cijeljenje rana u usporedbi s jažicama tretiranim kontrolama A, B i C. Upotrebom t testa između kontrole 2. dana i propolisom tretiranih stanica, utvrđena je statistički značajna razlika u cijeljenju rana (p<0,0032). Tijekom vremenskog perioda od 3 dana, statistički značajno je povećanje rasta tretiranih stanica propolisom u usporedbi s kontrolnom skupinom. Najbolje vrijednosti cijeljenja imale su stanice tretirane s 1,5% i 1% otopinom propolisa.Imunomodulatornim djelovanjem propolis povećava oslobađanje vazoaktivnih posrednika poput metabolita arahidonske kiseline. ELISA metodom izolacije interleukina 6 dokazan je statistički značajan porast IL-6 (p< 0.0086) između jažica tretiranih 1.5%, 1% i 0,5% otopinama propolisa te kontrolama A, B i C tijekom 3 dana. Propolis povećava oslobađanje citokina IL-6 i faktore rasta poput PDGF i FGF. Time je dokazan imunomodulatorni učinak propolisa u poticanju bržeg cijeljenja rana.

Abstract (english)

Propolis has immunomodulatory, antimicrobial, antioxidant, analgesic and anti-inflammatory effects, which promotes faster wound healing. Important compounds in propolis are flavonoids, phenylpropenoids and phenolic acids such as coumaric acid, caffeic acid and ferulic acid. Caffeic acid phenethyl ester (CAPE) has an immunosuppressive effect by inhibiting bacterial growth, preventing cell division. The antioxidant action of propolis is stimulated by the high concentration of polyphenols and flavonoids. Non-alcoholic propolis extract shows antimicrobial effect on gram positive bacteria and gram negative bacteria. Propolis shows an antifungal effect against fungi. MTT cytotoxicity test demonstrated the safety of propolis against cells. Considering the results of the scratch test, 1.5%, 1%, and 0.5% concentrated propolis solutions achieved statistically significantly faster wound healing compared to wells treated with controls A, B, and C. Using the t test between control day 2 and propolis treated cells, a statistically significant difference in wound healing was found (p <0.0032). Over a time period of 3 days, there was a statistically significant increase in the growth of propolis-treated cells compared to the control group. Cells treated with 1.5% and 1% propolis solution had the best healing values. By its immunomodulatory action, propolis increases the release of vasoactive mediators such as arachidonic acid metabolites. ELISA isolation of interleukin 6 showed a statistically significant increase in IL-6 (p <0.0086) between wells treated with 1.5%, 1% and 0.5% propolis solutions and controls A, B and C for 3 days. Propolis increases the release of the cytokine IL-6 and growth factors such as PDGF and FGF. This has proven the immunomodulatory effect of propolis in promoting faster wound healing.

• Keywords: Propolis; bezalkoholni ekstrakt; cijeljenje rana
• Keywords (english): propolis; non-alcoholic extract; wound healing
• Language: croatian
• URN:NBN: urn:nbn:hr:178:420856
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-10-19 08:34:41