Značenje dijagnostičkog pretraživanja zimskih ostataka s podnice košnice za zdravstveno stanje pčelinjih zajednica (Apis mellifera)

master's thesis

• Title (english): SIGNIFICANCE OF DIAGNOSTIC ANALYSES OF WINTER HIVE DEBRIS FOR THE HONEYBEE HEALTH CONDITION (Apis mellifera)
• Author: Klara Zubak-Novak
• Mentor: Ivana Tlak Gajger (mentor)
• Mentor: Metka Pislak Ocepek (mentor); VIAF: 315708052
• Committee member: Srebrenka Nejedli (predsjednik povjerenstva)
• Committee member: Metka Pislak Ocepek (član povjerenstva); VIAF: 315708052
• Committee member: Ivana Tlak Gajger (član povjerenstva)
• Committee member: Krešimir Matanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2021-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Animal Production and Biotechnology

Abstract

Bolesti pčela su jedan od najznačajnijih i najčešćih uzroka slabljenja i ugibanja pčelinjih zajednica. Rana dijagnostika kod subkliničkih infekcija uzročnicima bolesti pčelinje zajednice prioritet je za pravodobnu primjenu preventivnih i kontrolnih mjera u pčelarskoj praksi. Uzorkovanje ostataka s podnica košnica je jednostavna, neinvazivna i jeftina metoda, a pretraživanjem tih uzoraka pomoću molekularnih metoda PCR i qPCR testova može se brzo i pouzdano utvrditi prisutnost različitih uzročnika bolesti pčelinjih zajednica. Ovim radom su po prvi puta pretraženi uzorci zimskih ostataka s podnica košnica skupljeni na pčelinjacima u kontinentalnom djelu Republike Hrvatske primjenom PCR i qPCR metoda. Dobiveni rezultati laboratorijskog pretraživanja su analizirani i uspoređeni sa zdravstvenim stanjem i jačinom pčelinjih zajednica u proljeće. Potvrđeno je da se iz uzoraka ostataka s podnice košnice može pouzdano utvrditi i kvantificirati P. larvae, Nosema spp., virus akutne pčelinje paralize, virus crnih matičnjaka, virus izobličenih krila i virus mješinastog legla. Utvrđeno je da su pretražene pčelinje zajednice bile istodobno inficirane ili invadirane s prosječno četiri do šest uzročnika bolesti, te da su pojedine pčelinje zajednice u proljeće razvile kliničke znakove bolesti, a pojedine nisu preživjele zimu.

Abstract (english)

Honey bee diseases are one of the most significant and most common causes of bees weakness and death of the colonies. An early diagnosis of subclinical pathogens that cause diseases for performing precautionary and control measures. Sampling debris from beehive bottom boards is a simple, non-invasive and cheap method. By analysing these samples with molecular methods, PCR and qPCR tests, you can quickly and reliably determine the presence of various disease causing agents in the honey bee colony. In this thesis for the first time, samples from winter debris from the beehive bottom boards gathered from apiaries in the continental part of Croatia have been analysed by using the PCR and qPCR tests. Obtained laboratory results have been analysed and compared to the health condition and strength of honey bee colonies in spring. It has been confirmed that from the samples gathered from the beehive floors, we can reliably determine the presence and quantity of P. larvae, Nosema spp., Acute Bee Paralysis Virus (ABPV), Black Queen Cell Virus (BQCV), Deformed Wing Virus (DWV) and Sacbrood Virus (SBV). Observed honey bee colonies have been found to be infected or invaded at the same time, with an average of 4 to 6 disease carrying agents, and some og them have developed symptoms, while some didn't survive the winter at all.

• Keywords: pčelinja zajednica; uzorci zimskih ostataka s podnice košnica; PCR/qPCR; Nosema spp.; Paenibacillus larvae; Melissococcus plutonius; Crithidia mellificae; Lotmaria passim; virus akutne pčelinje paralize; virus mješinastog legla; virus izobličenih krila; virus crnih matičnjaka; Aethina tumida; Varroa destructor
• Keywords (english): honey bee colony; samples from winter hive debris; PCR/qPCR; Nosema spp.; Paenibacillus larvae; Melissococcus plutonius; Crithidia mellificae; Lotmaria passim; Acute Bee Paralysis Virus; Black Queen Cell Virus; Deformed Wing Virus and Sacbrood Virus; Aethina tumida; Varroa destructor
• Language: croatian
• URN:NBN: urn:nbn:hr:178:199617
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-12-14 10:47:15