DINAMIKA TITRA PROTUTIJELA PRIJE I NAKON PRIMJENE MS-H CJEPIVA U IMUNOPROFILAKSI BAKTERIJE Mycoplasma synoviae KOD JATA TEŠKE LINIJE KOKOŠI

master's thesis

• Title (english): DYNAMICS OF ANTIBODIES TITER BEFORE AND AFTER THE APPLICATION OF MSH VACCINE IN IMMUNOPROPHYLAXIS OF THE BACTERIA Mycoplasma synoviae IN A BROILER PARENT FLOCK
• Author: Matej Kolar
• Mentor: Željko Gottstein (mentor)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2022-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Mycoplasma synoviae predstavlja jednu od prijetnji u peradarstvu na globalnoj razini budući da
izaziva velike ekonomske gubitke. Primjena atenuiranog temperaturno osjetljivog MS-H cjepiva
jedna je od učinkovitih biosigurnosnih mjera kojom bi izbjegli takve gubitke. Princip djelovanja
MS-H cjepiva je kolonizacija gornjih dišnih puteva peradi sa manje patogenim sojem kako bi
spriječili prihvaćanje divljih patogenih sojeva. U ovom diplomskom radu opisano je istraživanje
kojim se utvrdila razina titra protutijela u kokoši teške pasmine nakon zaraze divljim sojem i nakon
cijepljenja MS-H cjepivom. Između dvije skupine nije postojala značajna razlika u titru protutijela
prilikom prvog testiranja. Nakon provedenog drugog testiranja zabilježeno je kako je kod kokoši
zaraženih divljim sojem došlo do smanjenja titra protutijela, a kod kokoši cijepljenih MS-H
cjepivom je porasta titar protutijela zbog mogućeg proboja zaštite. Rezultati istraživanja ukazuju
na aktivnu imunost postignutu cijepljenjem.

Abstract (english)

Mycoplasma synoviae is one of the global threats in poultry industry as it causes large economic
losses. The use of an attenuated temperature-sensitive MS-H vaccine is one of the effective
protective measures to avoid such losses. The principle of action of the MS-H vaccine is the
colonization of the upper respiratory tract of poultry with a less pathogenic strain to prevent the
adherence of wild pathogenic strains. This thesis describes a study that determined the level of
antibody titers in broiler breeder flocks after infection with wild-type strain and after vaccination
with MS-H vaccine. There was no significant difference in antibody titer between the two groups
during the first sampling. After conducting a second test, an antibody titer decreased in wild-type
infected chickens, while increase in antibody titers occurred in chickens vaccinated with the MSH
vaccine, as probable result of wild type infection. The results of the study indicate active
immunity achieved by vaccination.

• Keywords: Mycoplasma synoviae; MS-H; cijepljenje; titar protutijela; ELISA
• Keywords (english): mycoplasma synoviae; MS-H; vaccination; antibody titers; ELISE
• Language: croatian
• URN:NBN: urn:nbn:hr:178:166293
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-01-10 11:12:42