KONCENTRACIJA FUNKCIONALNIH BIOMARKERA KOBALAMINA I FOLNE KISELINE U SERUMU KOD PASA S KRONIĈNIM GASTROINTESTINALNIM POREMEĆAJIMA: RETROSPEKTIVNA STUDIJA

master's thesis

• Title (english): Serum concentrations of functional biomarkers folate and cobalamin in dogs with chronic enteropathies: a retrospective study
• Author: Elizabeta Pongrac
• Mentor: Martina Crnogaj (mentor)
• Mentor: Darko Grden (mentor)
• Committee member: Damjan Gračner (predsjednik povjerenstva)
• Committee member: Mirna Brkljačić (član povjerenstva)
• Committee member: Martina Crnogaj (član povjerenstva)
• Committee member: Blanka Beer Ljubić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2022-02-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Simtomi poremećaja gastrointestinalnog sustava jedni su od glavnih problema zbog kojih
vlasnik dovodi svog psa na pregled kod veterinara. Kronični gastrointestinalni problemi
zahtjevaju opseņnu dijagnostiku te potom ciljano liječenje. Uz standardne dijagnostičke
postupke posljednjeg desetljeća razvijaju se i druge pretrage poput biomarkera koji bi mogli
pomoći u dijagnostičkoj procjeni ili prognozi kod pacijenata sa kroničnim gastrointestinalnim
poremećajima. U ovom radu obraĎena su dva biomarkera: kobalamin i folna kiselina.
Arhiva Klinike za unutarnje bolesti Veterinarskog fakulteta Sveučilińta u Zagrebu pretraņena
je kako bi se obradili podatci svih pasa s kroničnim gastrointestinalnim problemima u
razdoblju od 1. siječnja 2017. do 1. rujna 2021.. Uz standardne pretrage kod svih pasa
uključenih u ovo istraņivanje morale su biti odreĎene i koncentracije biomarkera kobalamina
i folne kiseline. Postavljene kriterije istraņivanja ispunilo je 68 pasa. Najzastupljenije pasmine
s kroničnim gastrointestinalnim problemima bile su kriņanci, malteńki psić, njemački ovčar,
kavalirski ńpanijel kralja Charlesa, labrador retriver i lagotto romagnolo. Prosječno trajanje
simptoma bilo je oko 6 mjeseci. Najčeńći simptomi bili su povraćanje, proljev i mrńavljenje.
Najzastupljenije dijagnoze u istraņivanoj skupini bile su enteropatija koja reagira na liječenje
bazirano na promjeni hrane (FRE), upalna bolest crijeva (IBD), enteropatija koja reagira na
liječenje antibioticima (ARE), egzokrina insuficijencija guńterače (EPI) i limfom.
Hipoalbuminemiju je imalo 37% pasa (25/68), od toga je 36% pasa (9/25) imalo sniņenu
koncentraciju kobalamina. Rezultati sugeriraju da postoji povezanost izmeĎu specifičnih
promjena u serumskim koncentracijama kobalamina i folne kiseline i dijagnoza poput EPI u
(100%; 6/6), ARE (67%; 6/9), limfoma (60%; 3/5) te IBD-a (53%; 9/17). Zbog nepovoljne
prognoze eutanazirano je 5 od 68 pacijenata (7%), 4 od 5 pacijenata (80%) je u trenutku
eutanazije imalo izrazito sniņenu koncentraciju albumina, a 3/5 pacijenata (60%) je imalo
sniņenu koncentraciju kobalamina.
Specifične promjene u serumskim koncentracijama kobalamina i folne kiseline mogu dati
informacije o lokalizaciji bolesti crijeva. U istraņivanju je utvrĎena povezanost izmeĎu
promjena u serumskim koncentracijama kobalamina i folne kiseline i dijagnoza poput EPI,
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ARE, IBD i limfoma, ńto se moņe koristiti pri odabiru daljnjih dijagnostičkih postupaka.
Sniņena koncentracija kobalamina i albumina u serumu kod kroničnih gastrointestinalnih
poremećaja prati nepovoljnu prognozu bolesti.

Abstract (english)

Symptoms of the gastrointestinal disorders are one of the main problems due to which the
owner brings his dog to the vet. Chronic gastrointestinal problems require extensive
diagnostics fallowed by specific treatment. Cobalamin and folate are functional biomarkers of
gastrointestinal permeability and absorptive function. Variations in serum cobalamin and
folate concentrations can, because of their absorption in different parts of intestine (cobalamin
is absorbed in the distal small intestine and folate in proximal small intestine), indicate
localization and help in diagnosing a specific intestinal disease.
This study included 68 dogs that were admitted to the Clinic for Internal Diseases at the
Faculty of Veterinary Medicine of University of Zagreb from 1st of January 2017 to 1st of
September 2021 with chronic signs of gastrointestinal disease. All dogs included in the study
had their serum cobalamin and folate concentration measured. The most common breeds were
mixed breed, Maltese, German Shephard, Cavalier Spaniel of King Charles, Labrador
Retriver and Lagotto Romagnolo. The average duration of clinical signs was about six
months. The most common clinical signs were vomiting, diarrhea and weight loss. The most
common gastrointestinal diseases that interfered with cobalamin and folate metabolism were
IBD, FRE, ARE, lymphoma and EPI. Serum albumin concentration was decreased in 37% of
the dogs (25/68), and 36% of them (9 dogs) had decreased serum cobalamin concentration.
There is evidented correlation between changes in serum cobalamin and folate and EPI
(100%; 6/6), ARE (67%; 6/9), lymphoma (60%; 3/5) and IBD (53%; 9/17). Decreased serum
cobalamin and albumin concentrations in chronic gastrointestinal disorders is negative
prognostic indicator.

• Keywords: pas; kobalamin; folna kiseline; kronične enteropatije; egzokrina insuficijencija guńterače
• Keywords (english): dog; cobalamin; folate; chronic enteropathies; exocrine pancreatic insufficiency
• Language: croatian
• URN:NBN: urn:nbn:hr:178:575554
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-03-01 13:01:46