Primjena molekularno-genetskih metoda u poboljšanju plodnosti crne slavonske pasmine svinja

master's thesis

• Title (english): Applicability of Molecular Genetic Methods in Order to Improve the Fertility of Black Slavonian Breed
• Author: Antun Brkić
• Mentor: Anamaria Ekert Kabalin (mentor)
• Mentor: Denis Cvitković (mentor)
• Committee member: Sven Menčik
• Committee member: Anamaria Ekert Kabalin
• Committee member: Denis Cvitković
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Animal Husbandry); Zagreb
• Defense date and country: 2014-06-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Animal Production and Biotechnology

Abstract

Crna slavonska svinja, nekada vrlo cijenjena i gotovo jedina pasmina u Slavoniji, danas postoji u maloj populaciji. Skromnih prohtjeva u hranidbi i smještaju, namijenjena je uzgoju na otvorenom. Prasi 6-8 odojaka u leglu, i prema tome pripada u srednje plodne pasmine svinja. Odlikuje se visokom kvalitetom mesa, namijenjenom preradi u tradicijske suhomesnate proizvode. Zbog svojih kvaliteta predstavlja prioritet u proučavanju i očuvanju jedinstvenog genetskog potencijala. Cilj rada bio je izolirati DNA iz folikula dlake, provesti genotipizaciju polimorfizma prolaktinskog receptora krmača crne slavonske pasmine svinje i utvrditi eventualnu povezanost sa reproduktivnim pokazateljima na uzorku od 15 jedinki. Navedeno bi moglo pridonijeti unaprjeđenju selekcije plotkinja, koja se dosad zasnivala na fenotipskim pokazateljima. Nakon provedene izolacije DNA i umnažanja odsječaka PRLR, dobiveni PCR produkti podvrgnuti su djelovanju restrikcijskog enzima AluI, u svrhu utvrđivanja polimorfizma PRLR gena. Dobivena je jednaka raspodjela AA (40%) i BB (40%) homozigota, dok je AB heterozigot bio manje zastupljen (20%). Najveći broj ukupno oprasenih kao i živooprasenih odojaka imale su krmače AB genotipa, iako je kod BB homozigota zabilježena veća varijabilnost u veličini legla. Unatoč utvrđenim razlikama u veličini legla pojedinog genotipa, one nisu bile statistički značajne. Analizom ekonomičnosti proizvodnje tovljenika u poluintenzivnim uvjetima, prikazano je da je moguće ostvariti dobit unatoč slabijim reproduktivnim i proizvodnim sposobnostima crne slavonske svinje u usporedi s visokoplodnim mesnim pasminama i hibridima svinja.

Abstract (english)

Population of Black Slavonian pig, once highly valued and almost the only breed in Slavonia, has significantly decreased over the years. With modest demands of nutrition and housing, they are intendend for the breeding in the open. This breed belongs in the middle fertile breed with 6-8 piglets per litter. It is characterized by high quality meat, meant for processing in traditional dried meat products. Research and preservation of this unique genetic potential are priority because of its quality. The aim of the study was to isolate DNA from hair follicle samples of 15 Black Slavonian sows, to genotype polymorphism of PRLR receptor and evaluate its possible connection with reproductive traits. This could contribute to improvement of selection which previously was based on phenotypic indicators. After DNA isolation and multiplication of PRLR sequence, obtained PCR products were subjected to restriction enzyme AluI in order to determine the polymorphism of PRLR-gene. The results have shown even distribution of AA (40%) and BB (40%) homozygotes, while AB heterozygote was less frequent (20%). The largest number of total born and live born piglets was in AB genotype, although the BB homozygotes observed greater variability in litter size. Despite the determined differences in litter size between genotypes they were not statistically significant. Economic analysis of the production of fattening pigs in semi intensive conditions showed that is possible to make a profit despite lower reproductive and productive capacities of Black Slavonian pigs compared to highfertility meat breeds and crossbreeds.

• Keywords: Crna slavonska svinja; genotipizacija
• Keywords (english): Black Slavonian pig; genotype
• Language: croatian
• URN:NBN: urn:nbn:hr:178:236851
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2015-12-03 09:57:46