Praćenje distribucije fluordeoksiglukoze u kokošjem zametku pozitronskom emisijskom tomografijom

master's thesis

• Title (english): Detection of Fluordeoxiglucose Distribution in Chicken Embryos Using Positron Emission Tomography
• Author: Tomislav Vrgoč
• Mentor: Željko Gottstein (mentor)
• Committee member: Danijela Horvatek Tomić
• Committee member: Maja Lukač
• Committee member: Željko Gottstein
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Poultry Diseases with Clinic); Zagreb
• Defense date and country: 2015-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Animal Production and Biotechnology

Abstract

Primjena PET-a ima veliku važnost u svakodnevnoj dijagnostici i istraživanjima pri
cemu primjena upravo na laboratorijskim životinjama ima veliku važnost u razvoju iste.
No, kontinuirano se ukazuje potreba za novim laboratorijskim modelima koji ce bolje
odgovarati primjeni PET-a. Kokošji zameci su se u zadnjih nekoliko godina pokazali kao
potencijalni model za istraživanje u podrucju PET metode no nisu u potpunosti definirani
svi nacini i uvjeti primjene radiofarmaka na njima. U ovom istraživanju pokušali smo
istražiti mogucnost primjene fluordeoksiglukoze (18FDG), najcešce korištenog
radiofarmaka, na kokošjim zamecima teške pasmine 13. dana inkubacije. Navedeni
radiofarmak primijenjen je razlicitim postupcima, iv u krvne žile korioalantoisne
membrane (CAM), u sam zametak te nakapavanjem u zracnu komoricu. Po aplikaciji
uslijedilo je snimanje u trajanju od oko jednog sata, u slucaju iv primjene ili u zametak
odmah, a u slucaju nakapavanja nakon tzv. „uptake-a“ u trajanju od sat vremena. Nakon
snimanja uslijedila je rekonstrukcija, obrada i analiza dobivene slike. Rezultati istraživanja
su pokazali da je primjena FDG-a iv u krvne žile CAM-a dala najbolje rezultate, iako ne u
svim slucajevima aplikacije, dok su ostali nacini bili manje ucinkoviti. Iv primjenjen FDG
osigurao je brzu distribuciju i kvalitetno oslikavanje cijelog zametka i organa od interesa
(mozga, srca i bubrega). Potvrdena je potreba za kemijskom imobilizacijom zametaka
tijekom snimanja zbog stalnih pokreta. Potrebna su daljnja istraživanja u smjeru primjene
PET-a za oslikavanje promjena u kokošjem zametku, narocito nakon višednevne
inkubacije po primjeni mikrorganizama ili neoplasticnih stanica, cije ce pracenje upravo
omoguciti primjena u ranijoj dobi zametka od 13 dana.

Abstract (english)

PET scan has is highly important method in every day diagnostic and research in
which aplication on laboratory animals has a great importance in development of the same.
But, there is continuous need for new laboratory models which will suit better to PET scan
application. Chicken embryos showed to be model for PET scan application in last few
years, but without defined all possible methods and Chicken embryos have in recent years
proved to be an interesting model for research in the field of PET, but all the methods and
conditions of application of radiopharmaceuticals to them have not been fully defined. In
this study, we investigated the possibility of applying fluorodeoxyglucose (18FDG), the
most commonly used radiopharmaceutical, in chicken embryos of heavy breed on 13th
day of incubation. Mentioned radiopharmaceutical was applied by different methods, iv
into the blood vessels of chorioallantoic membrane (CAM), into the embryo or by drip into
the air chamber. Application was followed by recording during the period of about one
hour, in case of iv administration or into the embryo immediately, or in case of dripping
after one hour of uptake. PET scan was followed by reconstruction, processing and
analysis of the resulting images. The results showed that the iv application of FDG into the
CAM blood vessel gave the best results, although not in all cases of applications, while
other methods were less effective. IV applied FDG ensure the rapid dissemination and
high-quality imaging of whole embryo and organs of interest (brain, heart and kidney).
Confirmed is the need for chemical immobilization of embryos during recording because
of the constant movement. Further research in the direction of PET application for imaging
of changes in the chicken embryo is needed, especially after several days of incubation
after the application of microorganisms or neoplastic cells, which precise monitoring will
be enabled by application at an embryo early age of 13 days.

• Keywords: PET; fluordeoksiglukoze; jaja
• Keywords (english): PET; fluorodeoxyglucose; eggs
• Language: croatian
• URN:NBN: urn:nbn:hr:178:365365
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-01-18 09:18:43